한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제50권2호
  • /
  • Pages.310-318
  • /
  • 2022
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Protective Effect of Paulownia tomentosa Fruits in an Experimental Animal Model of Acute Lung Injury

  • Kim, Seong-Man (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Ok-Kyoung (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Min, Jae-Hong (Laboratory Animal Resources Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Park, Jin-Mi (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Doo-Young (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Sei-Ryang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Seung Jin (College of Pharmacy, Chungnam National University) ;
  • Ahn, Kyung-Seop (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Jae-Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 투고 : 2021.12.29
  • 심사 : 2022.05.10
  • 발행 : 2022.06.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The fruits of Paulownia tomentosa (Thunb.) (PT) Steud. have been reported to exert a variety of biological activities. A previous study confirmed that compounds isolated from PT fruits (PTF) exerted anti-inflammatory effects on TNF-α-stimulated airway epithelial cells. However, there is no report on the protective effects of PTF on acute lung injury (ALI). Here, we examined the ameliorative effects of PTF in an experimental animal model of lipopolysaccharide (LPS)-induced ALI. In ALI mice, increased levels of inflammatory cell influx were confirmed in the lungs of mice, and an increase of microphage numbers, TNF-α, IL-6 and MCP-1 production and protein content were detected in mouse bronchoalveolar lavage fluid. However, these increases were significantly reversed with PTF pretreatment. In addition, PTF inhibited the increased expression of iNOS and COX-2 in the lungs of ALI mice. Furthermore, the upregulation of MAPK and NF-κB activation was decreased in the lungs of ALI mice by PTF. In the in vitro experiment, PTF pretreatment exerted an anti-inflammatory effect by inhibiting the secretion of nitric oxide, TNF-α and IL-6 in LPS-stimulated RAW264.7 macrophages. Collectively, these results indicated that PTF has ameliorative effects on airway inflammation in an experimental animal model of ALI.

키워드

과제정보

This study was supported by grants from the Korea Research Institute of Bioscience and Biotechnology Research Initiative Program (KRIBB) of the Republic of Korea (grant. nos. KGM5522211 and KGS1232221).

참고문헌

  1. Li J, Wang K, Huang B, Li R, Wang X, Zhang H, et al. 2021. The receptor for advanced glycation end products mediates dysfunction of airway epithelial barrier in a lipopolysaccharides-induced murine acute lung injury model. Int. Immunopharmacol. 93: 107419. https://doi.org/10.1016/j.intimp.2021.107419
  2. Lee JW, Chun W, Lee HJ, Min JH, Kim SM, Seo JY, et al. 2021. The role of macrophages in the development of acute and chronic inflammatory lung diseases. Cells 10: 897. https://doi.org/10.3390/cells10040897
  3. Bhargava M, Wendt CH. 2012. Biomarkers in acute lung injury. Transl. Res. 159: 205-217. https://doi.org/10.1016/j.trsl.2012.01.007
  4. Li H, Yang T, Fei, Z. 2021. miR26a5p alleviates lipopolysaccharide-induced acute lung injury by targeting the connective tissue growth factor. Mol. Med. Rep. 23: 5.
  5. Laskin DL, Malaviya R, Laskin JD. 2019. Role of macrophages in acute lung injury and chronic fibrosis induced by pulmonary toxicants. Toxicol. Sci. 168: 287-301. https://doi.org/10.1093/toxsci/kfy309
  6. Min JH, Kim SM, Park JW, Kwon NH, Goo SH, et al. 2021. Lagerstroemia ovalifolia exerts anti-inflammatory effects in mice of LPS induced ALI via downregulating of MAPK and NF-κB activation. J. Microbiol. Biotechnol. 31: 1501-1507. https://doi.org/10.4014/jmb.2107.07023
  7. Guimaraes LMF, Rossini CVT, Lameu C. 2021. Implications of SARS-Cov-2 infection on eNOS and iNOS activity: Consequences for the respiratory and vascular systems. Nitric Oxide. 111-112: 64-71. https://doi.org/10.1016/j.niox.2021.04.003
  8. Giuliano F, Warner TD. 2002. Origins of prostaglandin E2: involvements of cyclooxygenase (COX)-1 and COX-2 in human and rat systems. J. Pharmacol. Exp. Ther. 303: 1001-1006. https://doi.org/10.1124/jpet.102.041244
  9. Park JW, Ryu HW, Ahn HI, Min JH, Kim SM, Kim MG, et al. 2020. The anti-inflammatory effect of Trichilia martiana C. DC. in the Lipopolysaccharide-stimulated inflammatory response in macrophages and airway epithelial cells and in LPS-challenged mice. J. Microbiol. Biotechnol. 30: 1614-1625. https://doi.org/10.4014/jmb.2006.06042
  10. Ding H, Ci X, Cheng H, Yu Q, Li D. 2019. Chicoric acid alleviates lipopolysaccharide-induced acute lung injury in mice through anti-inflammatory and anti-oxidant activities. Int. Immunopharmacol. 66: 169-176. https://doi.org/10.1016/j.intimp.2018.10.042
  11. Yang S, Yu Z, Wang L, Yuan T, Wang X, Zhang X, et al. 2017. The natural product bergenin ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NF-κB activition. J. Ethnopharmacol 200: 147-155. https://doi.org/10.1016/j.jep.2017.02.013
  12. Min JH, Kim MG, Kim SM, Park JW, Chun W, Lee HJ, et al. 2020. 3,4,5-Trihydroxycinnamic acid exerts a protective effect on pulmonary inflammation in an experimental animal model of COPD. Int. Immunopharmacol. 85: 106656. https://doi.org/10.1016/j.intimp.2020.106656
  13. Lee JW, Chun W, Kwon OK, Park HA, Lim Y, Lee JH, et al. 2018. 3,4,5-Trihydroxycinnamic acid attenuates lipopolysaccharide (LPS)-induced acute lung injury via downregulating inflammatory molecules and upregulating HO-1/AMPK activation. Int. Immunopharmacol. 64: 123-130. https://doi.org/10.1016/j.intimp.2018.08.015
  14. Navratilova A, Schneiderova K, Vesela D, Hanakova Z, Fontana A, Dall'Acqua S, et al. 2013. Minor C-geranylated flavanones from Paulownia tomentosa fruits with MRSA antibacterial activity. Phytochemistry 89: 104-113. https://doi.org/10.1016/j.phytochem.2013.01.002
  15. Ryu HW, Park YJ, Lee SU, Lee S, Yuk HJ, Seo KH. et al. 2017. Potential anti-inflammatory effects of the fruits of Paulownia tomentosa. J. Nat. Prod. 80: 2659-2665. https://doi.org/10.1021/acs.jnatprod.7b00325
  16. Ali SA, Ibrahim NA, Mohammed MMD, El-Hawary S, Refaat EA. 2019. The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis. Heliyon 5: e01769. https://doi.org/10.1016/j.heliyon.2019.e01769
  17. Lee JW, Ryu HW, Kim DY, Kwon OK, Jang HJ, Kwon HJ, et al. 2021. Biflavonoid-rich fraction from Daphne pseudomezereum var. koreana Hamaya exerts anti-inflammatory effect in an experimental animal model of allergic asthma. J. Ethnopharmacol. 265: 113386. https://doi.org/10.1016/j.jep.2020.113386
  18. Kim SM, Min JH, Kim JH, Choi J, Park JM, Lee J, et al. 2022. Methyl p-hydroxycinnamate exerts anti-inflammatory effects in mouse models of lipopolysaccharide-induced ARDS. Mol. Med. Rep. 25: 37.
  19. Kim SM, Ryu HW, Kwon OK, Hwang D, Kim MG, Min JH, et al. 2021. Callicarpa japonica Thunb. ameliorates allergic airway inflammation by suppressing NF-κB activation and upregulating HO-1 expression. J. Ethnopharmacol. 267: 113523. https://doi.org/10.1016/j.jep.2020.113523
  20. Kwon OK, Lee JW, Xuezhen X, Harmalkar DS, Song JG, Park JW, et al. 2020. DK-1108 exerts anti-inflammatory activity against phorbol 12-myristate 13-acetate-induced inflammation and protective effect against OVA-induced allergic asthma. Biomed. Pharmacother. 132: 110950. https://doi.org/10.1016/j.biopha.2020.110950
  21. Huang X, Xiu H, Zhang S, Zhang G. 2018. The role of macrophages in the pathogenesis of ALI/ARDS. Mediators Inflamm. 2018: 1264913. https://doi.org/10.1155/2018/1264913
  22. Chen X, Tang J, Shuai W, Meng J, Feng J, Han Z. 2020. Macrophage polarization and its role in the pathogenesis of acute lung injury/acute respiratory distress syndrome. Inflamm. Res. 69: 883-895. https://doi.org/10.1007/s00011-020-01378-2
  23. He Q, Wang Y, Yang H, Wang J, Zhang J, Liu D. 2021. Apelin36 protects against lipopolysaccharide-induced acute lung injury by inhibiting the ASK1/MAPK signaling pathway. Mol. Med. Rep. 23: 6.
  24. Yang L, Luo W, Zhang Q, Hong S, Wang Y, Samorodov AV, et al. 2021. Cardamonin inhibits LPS-induced inflammatory responses and prevents acute lung injury by targeting myeloid differentiation factor 2. Phytomedicine 93: 153785. https://doi.org/10.1016/j.phymed.2021.153785
  25. Badamjav R, Sonom D, Wu Y, Zhang Y, Kou J, Yu B, et al. 2020. The protective effects of Thalictrum minus L. on lipopolysaccharide-induced acute lung injury. J. Ethnopharmacol. 248: 112355. https://doi.org/10.1016/j.jep.2019.112355
  26. Ryu HW, Lee JW, Kim MO, Lee RW, Kang MJ, Kim SM, et al. 2022. Daphnodorin C isolated from the stems of Daphne kiusiana Miquel attenuates airway inflammation in a mouse model of chronic obstructive pulmonary disease. Phytomedicine 96: 153848. https://doi.org/10.1016/j.phymed.2021.153848
  27. Lee JW, Kim MO, Song YN, Min JH, Kim SM, Kang MJ, et al. 2021. Compound K ameliorates airway inflammation and mucus secretion through the regulation of PKC signaling in vitro and in vivo. J. Ginseng Res. 46: 496-504.
  28. Kim MO, Lee JW, Lee JK, Song YN, Oh ES, Ro H, et al. 2022. Black ginseng extract suppresses airway inflammation induced by cigarette smoke and lipopolysaccharides in vivo. Antioxidants 11: 679. https://doi.org/10.3390/antiox11040679