한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제22권1호
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  • Pages.30-37
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  • 2022
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  • 1976-9334(pISSN)
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  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
권호 표지
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3T3-L1 전구지방세포에서 개구리자리(Ranunculus sceleratus) 추출물의 AMPK 신호전달을 통한 지방생성 억제 효과

Extract of Ranunculus sceleratus Reduced Adipogenesis by Inhibiting AMPK Pathway in 3T3-L1 Preadipocytes

  • 김예지 (충남대학교 수의과대학 조직학교실) ;
  • 조성필 (충남대학교 수의과대학 조직학교실) ;
  • 이희주 (충남대학교 수의과대학 조직학교실) ;
  • 홍금란 (충남대학교 수의과대학 조직학교실) ;
  • 김경현 (충남대학교 수의과대학 조직학교실) ;
  • 류시윤 (충남대학교 수의과대학 해부학교실) ;
  • 정주영 (충남대학교 수의과대학 조직학교실)
  • Kim, Yae-Ji (Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Sung-Pil (Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Hui-Ju (Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Hong, Geum-Lan (Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Kyung-Hyun (Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Ryu, Si-Yun (Department of Anatomy & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Jung, Ju-Young (Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University)
  • 투고 : 2022.05.06
  • 심사 : 2022.06.08
  • 발행 : 2022.06.30
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초록

Objectives: Adipogenesis is the process by which pre-adipocytes are differentiated into adipocytes. It also plays an important role in adipocyte formation and lipid accumulation. Ranunculus sceleratus (R. sceleratus) extracts are used for the treatment of various diseases such as hepatitis, jaundice, and tuberous lymphadenitis in oriental medicine. However, its effect on adipogenesis has not yet been studied. In this study, we investigated the effects of R. sceleratus on adipogenesis in 3T3-L1 cells. Methods: Cells were treated with 50, 100, and 200 ㎍/ml of R. sceleratus and cell viability was evaluated. To differentiate the 3T3-L1 preadipocytes, a 3-isobutyl-1-methylxanthine, dexamethasone, and insulin (MDI) solution were used. The accumulation of lipid droplets was determined by Oil Red O staining. The expression levels of adipogenesis-related proteins were also determined. Results: MDI solution differentiated the preadipocytes into adipocytes and accumulation of lipids was observed in the differentiated 3T3-L1 cells. Interestingly, the amount of lipid droplets was reduced after R. sceleratus treatment. In addition, the expression levels of key adipogenic transcription factors, such as CCAAT/enhancer-binding proteins-𝛼 (C/EBP-𝛼) and peroxisome proliferator-activated receptors-𝛾 (PPAR-𝛾) were also reduced after R. sceleratus treatment. Furthermore, R. sceleratus increased AMP-activated kinase (AMPK) phosphorylation and decreased sterol regulatory element-binding protein-1 expression. Conclusions: Our results showed that R. sceleratus reduced preadipocyte differentiation by inhibiting C/EBP-𝛼 and PPAR-𝛾 levels via the AMPK pathway. Therefore, we suggest that R. sceleratus may be potentially used as an anti-adipogenic agent.

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