Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-inflammatory effect of soil blue-green algae Nostoc commune isolated from Daejeon National Cemetery

국립대전현충원에서 분리한 남조류 구슬말(Nostoc commune)의 항염증 효과

  • Hong, Hyehyun (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Bae, Eun Hee (Microorganism Resources Division, Biological Resources Research Department, National Institute of Biological Resources) ;
  • Park, Tae-Jin (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kang, Min-Sung (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kang, Jae Shin (Microorganism Resources Division, Biological Resources Research Department, National Institute of Biological Resources) ;
  • Chi, Won-Jae (Microorganism Resources Division, Biological Resources Research Department, National Institute of Biological Resources) ;
  • Kim, Seung-Young (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
  • Received : 2022.03.17
  • Accepted : 2022.05.25
  • Published : 2022.06.30
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Abstract

We examined the anti-inflammatory properties of Nostoc commune HCW0811 in lipopolysaccharide-stimulated RAW264.7 macrophage cells. The anti-inflammatory activity of HCW0811 on viability of treated cells was assessed by measuring the level of expression of NO, prostaglandin E2 and pro-inflammatory cytokines, namely interleukin-1β, interleukin-6, and tumor necrosis factor-α in HCW0811 treated RAW 264.7 macrophages. HCW0811 was non-toxic to cells and inhibited the production of cytokines in a concentration-dependent manner. In addition its treatment suppressed the production of pro-inflammatory cytokines in a dose-dependent manner, and concomitantly decreased the protein expressions of inducible NO synthase and cyclooxygenase-2. Moreover, the levels of the phosphorylation of mitogen-activated protein kinase family proteins such as extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, and nuclear factor kappa B were reduced by HCW0811. These findings suggest that the HCW0811 collected from Daejeon National Cemetery have anti-inflammatory effects, and demonstrated its efficacy in cell-based in vitro assays.

구슬말(Nostoc commune Vaucher ex Bornet & Flahault)은 이형세포를 갖는 특징으로 다른 목들과 구분되는 남조류의 일종으로 극지방에서 열대지역까지 광범위한 지역에 분포하며 단세포가 연결되어 형성된 수많은 trichome들이 점액질에 둘러 쌓인 형태로 커다란 군체를 형성한다. 주로 토양, 암반, 잔디 위 등에 서식한다고 알려져 있으나 흔히 관찰되지 않기 때문에 현재 연구가 거의 없는 실정이다. 이에 본 연구에서는 토양 남조류인 N. commune HCW0811을 분리 및 동정하였으며 항염증 활성을 조사 하고자 하였다. 그 결과 N. commune HCW0811는 LPS로 유도된 RAW 264.7세포에서 80%이상의 세포 생존율을 나타내었으며 NO, PGE2 및 TNF-α, IL-6, IL-1β의 생성을 효과적으로 억제하였다. 또한 western blot assay를 통해 iNOS, COX-2 및 MAP kinase (p38, ERK1/2, JNK)와 NF-κB 세포내 신호전달 경로에서의 단백질 발현을 조사한 결과 이들의 발현이 유의하게 억제됨을 확인하였다. 본 연구에서는 이러한 결과를 근거하여 HCW0811가 다양한 염증 인자를 표적으로 하는 피부 면역 질환을 포함한 염증성 질환의 예방과 치료를 위한 항염증 기능성 화장품 및 식품소재로의 개발가능성을 제시한다.

Keywords

Acknowledgement

본 논문은 정부 (환경부)의 재원으로 국립생물자원관의 지원을 받아 수행하였습니다(NIBR202112101).

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