Journal of Applied Biological Chemistry

  • 제65권2호
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  • Pages.101-105
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  • 2022
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Quercetin의 식물병원성 세균인 Xanthomonas campestris에 대한 선택적 살균활성

Selective antibacterial activity of quercetin against Xanthomonas campestris

  • Kim, Jeong Yoon (Department of Pharmaceutical Engineering, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Kim, Ju Yeon (Department of Pharmaceutical Engineering, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Seo, Sung-Jun (Department of Agriculture Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Seo, Min-Kyung (Eco-Friendly Agri-Bio Research Center, Jeonam Bioindustry Foundation) ;
  • Kim, Jin-Seong (Department of Agriculture Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Kang, Sang-Woo (Department of Agriculture Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Oh, Kyeong-Yeol (Department of Agriculture Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Kim, Jin-Hyo (Department of Agriculture Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
  • 투고 : 2022.04.07
  • 심사 : 2022.05.17
  • 발행 : 2022.06.30
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초록

양파의 주요 flavonoid 성분인 quercetin에 대한 항균 활성은 일부 알려져 있으나, 식물 병원성 세균인 X. campestris와 E. carotovora에 대한 활성은 알려져 있지 않았다. 본 연구에서는 quercetin과 양파 추출물을 활용하여 이들 식물병원균에 대한 항균 활성과 농용 항생제인 streptomycin과의 synergistic 효과를 평가하였으나, E. carotovora에 대한 항균 활성은 없었다. 하지만, X. campestris에서는 다른 세균류에서 알려진 MIC보다 10배 이상 낮은 15.6 ㎍ mL-1이었으며, MBC는 20.0 ㎍ mL-1으로 X. campestris에 대해 높은 선택적 항균 활성을 확인할 수 있었다. 다만, 농업용 항생제로 사용중인 streptomycin과의 약효상승 효과는 없었다. 또한, quercetin은 양파껍질의 EtOAc와 EtOH 추출물로부터 확보할 수 있었으며, EtOAc 추출물에서 quercetin 순도가 높음을 확인하였고, 이에 따라 EtOH 추출물보다 2배 가량 높은 항균 활성(MIC = 500 ㎍ mL-1)이 확인되었다.

Quercetin is a major flavonoid in onion and known to antimicrobial activity against several pathogenic bacteria. However, the antibacterial activity of quercetin had not been evaluated against plant pathogenic Xanthomonas campestris and Erwinia carotovora. In here, quercetin and the solvent extracts of onion were investigated their antibacterial activity. Quercetin showed a selective inhibitory activity against X. campestris, and the minimal inhibition concentration (MIC) and minimal bactericidal concentration of quercetin were 15.6 and 20.0 ㎍ mL-1 respectively. Otherwise, it showed no inhibitory activity against E. carotovora, and no the additive and the synergistic effects with streptomycin on X. campestris. And the EtOAc extract from the peel of onion that contained quercetin showed 2-fold lower MIC (500 ㎍ mL-1) than the EtOH extract, thus EtOAc was suggested as the extraction solvent for quercetin from onion peel.

키워드

과제정보

This study was carried out with the support of "Research Program for Agricultural Science & Technology Development (PJ01571605)", Rural Development Administration, Republic of Korea.

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