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Establishment of withdrawal time and analysis of dexamethasone residue in milk of intramuscularly dosed cows

우유 중 dexamethasone의 잔류 분석 및 휴약기간 설정 연구

  • Park, Kwangil (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Jin, Yeung Bae (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Woohyun (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University)
  • 박광일 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 진영배 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 김우현 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 김석 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 이후장 (경상국립대학교 수의과대학 및 동물의학연구소)
  • Received : 2022.04.22
  • Accepted : 2022.06.09
  • Published : 2022.06.30

Abstract

This study investigated dexamethasone (DXM) residues in the milk from intramuscularly dosed dairy cows and established the withdrawal time (WT) of DXM in milk. Eighteen healthy Holstein cows were injected with 20 (DXM-1) or 40 mL (DXM-2) of a drug containing 1 mg/mL of DXM. After administering DXM, milk samples were collected from all cows at 12-hour intervals for five days. The DXM residue concentrations in milk were determined by liquid chromatography-tandem mass spectrometry. The correlation coefficient of the calibration curve was 0.9966, and the limits of detection and quantification (LOQ) were 0.03 and 0.1 ㎍/kg, respectively. The recoveries were 97.0% to 104.0%, and the coefficient of variations was less than 7.22%. After treatment, DXM in DXM-1 was detected above the LOQ in two milk samples at 36 hours and below the LOQ in all milk samples of DXM-2 at 48 hours. Using the WT calculation program WT 1.4, the withdrawal periods of DXM-1 and DXM-2 in milk were established to be two days. In conclusion, the developed analytical method is sensitive and reliable for detecting DXM in milk. The estimated WT of DXM in bovine milk is shorter than the current milk WT recommendation of three days for DXM in lactating dairy cows.

Keywords

Acknowledgement

본 논문은 가축위생방역본부(2020년 동물용의약품 안전관리기준 마련을 위한 잔류성 시험분석)의 지원에 의해 수행되었으며, 이에 감사드립니다.

References

  1. Rana MS, Lee SY, Kang HJ, Hur SJ. Reducing veterinary drug residues in animal products: a review. Food Sci Anim Resour 2019;39:687-703. https://doi.org/10.5851/kosfa.2019.e65
  2. Beyene T. Veterinary drug residues in food-animal products: its risk factors and potential effects on public health. J Vet Sci Technol 2016;7:1-7.
  3. Baynes RE, Dedonder K, Kissell L, Mzyk D, Marmulak T, Smith G, Tell L, Gehring R, Davis J, Riviere JE. Health concerns and management of select veterinary drug residues. Food Chem Toxicol 2016;88:112-122. https://doi.org/10.1016/j.fct.2015.12.020
  4. Jeong SH, Kang D, Lim MW, Kang CS, Sung HJ. Risk assessment of growth hormones and antimicrobial residues in meat. Toxicol Res 2010;26:301-313. https://doi.org/10.5487/TR.2010.26.4.301
  5. Malekinejad H, Rezabakhsh A. Hormones in dairy foods and their impact on public health: a narrative review article. Iran J Public Health 2015;44:742-758.
  6. Khaniki GR. Chemical contaminants in milk and public health concerns: a review. Int J Dairy Sci 2007;2:104-115. https://doi.org/10.3923/ijds.2007.104.115
  7. Perez-Cornago A. Commentary: Dairy milk intake and breast cancer risk: does an association exist, and what might be the culprit? Int J Epidemiol 2020;49:1537-1539. https://doi.org/10.1093/ije/dyaa199
  8. Yahi D, Ojo NA, William A, Uchendu C, Adebayo A, Mshelia GD. The use of dexamethasone in animals: implication for fertility, pregnancy and extrapolation of the animal data to humans. Niger Vet J 2017;38:83-103.
  9. Cherlet M, De Baere S, De Backer P. Quantitative determination of dexamethasone in bovine milk by liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2004;805:57-65. https://doi.org/10.1016/j.jchromb.2004.02.015
  10. Vincenti M, Girolami F, Capra P, Pazzi M, Carletti M, Gardini G, Nebbia C. Study of dexamethasone urinary excretion profile in cattle by LC-MS/MS: comparison between therapeutic and growth-promoting administration. J Agric Food Chem 2009;57:1299-1306. https://doi.org/10.1021/jf803465d
  11. Oh JH, Kwon CH, Jeon JS, Choi DM. Management of veterinary drug residues in food. Korean J Environ Agric 2009;28: 310-325. https://doi.org/10.5338/KJEA.2009.28.3.310
  12. Ministry of Food and Drug Safety (MFDS). Press Release: Reinforcement of Veterinary Drug Residue Management for Livestock and Fishery Products. MFDS, Cheongsu, 2020.
  13. Animal and Plant Quarantine Agency (APQA). Press Release: Preliminary Preparation for the Introduction of the Positive List System for Livestock Products. APQA, Gimcheon, 2022.
  14. Ministry of Food and Drug Safety (MFDS). Food Code. Chapter 8. General Test Methods. 8. Residue Analytical Methods for Veterinary Drugs in Food. 8.3.33. Dexamethasone. MFDS, Cheongju, 2016.
  15. Cha CN, Park EK, Yoo CY, Lee SJ, Son SE, Kim S, Lee HJ. Development of an analytical method for the determination of dexamethasone in bovine milk using liquid chromatography coupled to tandem mass spectrometry. J Food Hyg Saf 2017; 32:418-423. https://doi.org/10.13103/JFHS.2017.32.5.418
  16. Ministry of Food and Drug Safety (MFDS). Practical Handbook for Analysis of Residual Veterinary Drugs. pp. 48-59, MFDS, Cheongju, 2014.
  17. European Medicines Agency (EMA). Guideline on Determination of Withdrawal Periods for Milk. EMA, Amsterdam, 2021.
  18. Ural MN, Kotan A. A simple and rapid LC-MS/MS method for determination of dexamethasone in bovine milk. Mac Vet Rev 2020;43:69-73. https://doi.org/10.2478/macvetrev-2020-0014
  19. Li C, Wu Y, Yang T, Zhang Y. Rapid simultaneous determination of dexamethasone and betamethasone in milk by liquid chromatography tandem mass spectrometry with isotope dilution. J Chromatogr A 2010;1217:411-414. https://doi.org/10.1016/j.chroma.2009.12.015
  20. Codex Alimentarius. Guidelines for the Design and Implementation of National Regulatory Food Safety Assurance Programme Associated with the Use of Veterinary Drugs in Food Producing Animals CAC/GL 71. Food and Agriculture Organization of the United Nations, Rome, 2009.
  21. Ministry of Food and Drug Safety (MFDS). Food Code. Appendix 6. Maximum Residue Limits of Veterinary Drugs in Food. MFDS, Cheongju, 2017.
  22. Tainturier D, Alvinerie M, Brandon RA, Toutain PL. Dexamethasone concentrations in bovine blood plasma and milk after intravenous injection. J Dairy Sci 1982;65:1921-1924. https://doi.org/10.3168/jds.s0022-0302(82)82439-9
  23. Reding J, Sahin A, Schlatter J, Naegeli H. Dexamethasone and flumethasone residues in milk of intramuscularly dosed cows. J Vet Pharmacol Ther 1997;20:198-203. https://doi.org/10.1111/j.1365-2885.1997.tb00095.x
  24. The European Agency for the Evaluation of Medical Products (EMEA). Committee for Veterinary Medicinal Products, Dexamethasone, Summary Report (2) (EMEA/MRL/195/97-CORRIGENDUM). EMEA, London, 1997.
  25. Caloni F, Belloli C, Crescenzo G, Ormas P, Archimbault P. Determination of dexamethasone in milk of dairy cows by immuno-enzymatic assay. Vet Hum Toxicol 2000;42:345-348.
  26. Animal and Plant Quarantine Agency (APQA). Veterinary Drugs Management System-Electronic Civil Complaint Application. APQA, Gimcheon, 2022.