Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Inhibitory Effect of Ceylon Black Tea Extract on the Melanogenesis in 𝛼-MSH Stimulated B16F10 Melanoma Cells

  • Received : 2022.02.23
  • Accepted : 2022.02.28
  • Published : 2022.06.30
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Abstract

The desire to be light skinned is universal among women. Asia has a long history of using skincare formulations as whitening agents. There is an imperative need to develop novel cosmetics from herbal sources due to several unpleasant side effects and high costs. As a result, this study aims to investigate the effect of Ceylon black tea extracts on melanogenesis. Five different Ceylon black tea extracts were prepared and examined for total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity, and tyrosinase inhibitory activity. Furthermore, B16F10 melanoma cells were treated with these extracts and tested for cytotoxicity and protein suppression levels. According to the results of this study, the highest TPCs were obtained from ethanol and acetone extractions (240.303 ± 1.389 ㎍/g and 240.202 ± 4.700 ㎍/g, respectively), whereas the highest TFC was obtained from acetone extraction (57.484 ± 0.413 ㎍/g). Ceylon black tea extracted with ethanol exhibited the highest inhibitory activity on tyrosinase with an IC50 value of 0.277 ± 0.017 mg/mL and the highest DPPH radical scavenging activity with an EC50 value of 0.009 ± 0.000 mg/mL. Furthermore, western blot results revealed that tyrosinase, TRP-1, TRP-2, and MITF protein expression levels were dose-dependently suppressed, indicating the applicability of Ceylon black tea extract as a novel melanogenesis inhibitor.

Keywords

Acknowledgement

This study was supported by 2019 Academic Research Support Program in Gangneung-Wonju National University, Gangneung, 25457, Republic of Korea

References

  1. Perva-Uzunalic, A., Skerget, M., Knez, Z., Weinreich, B., Otto, F. and Gruner, S., 2006. Extraction of active ingredients from green tea (Camellia sinensis): Extraction efficiency of major catechins and caffeine. Food chemistry, 96(4), pp.597-605. https://doi.org/10.1016/j.foodchem.2005.03.015
  2. Naveed, M., BiBi, J., Kamboh, A.A., Suheryani, I., Kakar, I., Fazlani, S.A., FangFang, X., Yunjuan, L., Kakar, M.U., Abd El-Hack, M.E. and Noreldin, A.E., 2018. Pharmacological values and therapeutic properties of black tea (Camellia sinensis): A comprehensive overview. Biomedicine & Pharmacotherapy, 100, pp.521-531. https://doi.org/10.1016/j.biopha.2018.02.048
  3. Jayasekera, S., Molan, A.L., Garg, M. and Moughan, P.J., 2011. Variation in antioxidant potential and total polyphenol content of fresh and fully-fermented Sri Lankan tea. Food chemistry, 125(2), pp.536-541. https://doi.org/10.1016/j.foodchem.2010.09.045
  4. Nibir, Y.M., Sumit, A.F., Akhand, A.A., Ahsan, N. and Hossain, M.S., 2017. Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh. Asian Pacific Journal of Tropical Biomedicine, 7(4), pp.352-357. https://doi.org/10.1016/j.apjtb.2017.01.005
  5. Malongane, F., McGAW, L.J. and Mudau, F.N., 2017. The synergistic potential of various teas, herbs and therapeutic drugs in health improvement: a review. Journal of the Science of Food and Agriculture, 97(14), pp.4679-4689. https://doi.org/10.1002/jsfa.8472
  6. Sanlier, N., Gokcen, B.B. and Altug, M., 2018. Tea consumption and disease correlations. Trends in Food Science & Technology, 78, pp.95-106. https://doi.org/10.1016/j.tifs.2018.05.026
  7. Miller, P.E., Zhao, D., Frazier-Wood, A.C., Michos, E.D., Averill, M., Sandfort, V., Burke, G.L., Polak, J.F., Lima, J.A., Post, W.S. and Blumenthal, R.S., 2017. Associations of coffee, tea, and caffeine intake with coronary artery calcification and cardiovascular events. The American journal of medicine, 130(2), pp.188-197. https://doi.org/10.1016/j.amjmed.2016.08.038
  8. Heber, D., Zhang, Y., Yang, J., Ma, J.E., Henning, S.M. and Li, Z., 2014. Green tea, black tea, and oolong tea polyphenols reduce visceral fat and inflammation in mice fed high-fat, high-sucrose obesogenic diets. The Journal of nutrition, 144(9), pp.1385-1393. https://doi.org/10.3945/jn.114.191007
  9. Singh, B.N., Prateeksha, Rawat, A.K.S., Bhagat, R.M. and Singh, B.R., 2017. Black tea: Phytochemicals, cancer chemoprevention, and clinical studies. Critical reviews in food science and nutrition, 57(7), pp.1394-1410. https://doi.org/10.1080/10408398.2014.994700
  10. Ratnasooriya, W.D., Jayakody, J.R.A.C., Rosa, S.R.D. and Ratnasooriya, C.D.T., 2014. In vitro sun screening activity of Sri Lankan orthodox black tea (Camellia sinensis linn). World Journal of Pharmaceutical Sciences, pp.144-148.
  11. Ratnasooriya, W.D., Abeysekera, W.P.K.M. and Ratnasooriya, C.D.T., 2014. In vitro skin whitening and lightening properties of Sri Lankan low grown orthodox Orange Pekoe grade black tea. World Journal of Pharmaceutical Sciences, pp.1249-1252.
  12. Ratnasooriya, W.D., Abeysekera, W.P.K.M. and Ratnasooriya, C.T.D., 2014. In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.). Asian Pacific Journal of Tropical Biomedicine, 4(12), pp.959-963. https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0462
  13. Lee, J.K. and Byun, H.G., 2018. A novel BACE inhibitor isolated from Eisenia bicyclis exhibits neuroprotective activity against β-amyloid toxicity. Fisheries and Aquatic Sciences, 21(1), pp.1-9. https://doi.org/10.1186/s41240-017-0078-4
  14. Xu, J.G., Tian, C.R., Hu, Q.P., Luo, J.Y., Wang, X.D. and Tian, X.D., 2009. Dynamic changes in phenolic compounds and antioxidant activity in oats (Avena nuda L.) during steeping and germination. Journal of agricultural and food chemistry, 57(21), pp.10392-10398. https://doi.org/10.1021/jf902778j
  15. Bag, G.C., Devi, P.G. and Bhaigyabati, T.H., 2015. Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur valley. International Journal of Pharmaceutical Sciences Review and Research, 30(1), pp.154-159.
  16. Blois, M.S., 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), pp.1199-1200. https://doi.org/10.1038/1811199a0
  17. No, J.K., Kim, Y.J., Shim, K.H., Jun, Y.S., Rhee, S.H., Yokozawa, T. and Chung, H.Y., 1999. Inhibition of tyrosinase by green tea components. Life sciences, 65(21), pp.PL241-PL246.
  18. Hansen, M.B., Nielsen, S.E. and Berg, K., 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. Journal of immunological methods, 119(2), pp.203-210. https://doi.org/10.1016/0022-1759(89)90397-9
  19. Rathnayake, A.U., Abuine, R., Palanisamy, S., Lee, J.K. and Byun, H.G., 2021. Characterization and purification of β- secretase inhibitory peptides fraction from sea cucumber (Holothuria spinifera) enzymatic hydrolysates. Process Biochemistry, 111, pp.86-96. https://doi.org/10.1016/j.procbio.2021.10.007
  20. Jin, W., Wang, W.Y., Zhang, Y.L., Yang, Y.J., Chu, Q.C. and Ye, J.N., 2013. Determination of phenolic whitening agents in cosmetics by micellar electrokinetic capillary chromatography with amperometric detection. Chinese Chemical Letters, 24(7), pp.636-638. https://doi.org/10.1016/j.cclet.2013.04.023
  21. Ko, H.H., Chiang, Y.C., Tsai, M.H., Liang, C.J., Hsu, L.F., Li, S.Y., Wang, M.C., Yen, F.L. and Lee, C.W., 2014. Eupafolin, a skin whitening flavonoid isolated from Phyla nodiflora, downregulated melanogenesis: Role of MAPK and Akt pathways. Journal of ethnopharmacology, 151(1), pp.386-393. https://doi.org/10.1016/j.jep.2013.10.054
  22. Meerloo, J.V., Kaspers, G.J. and Cloos, J., 2011. Cell sensitivity assays: the MTT assay. In Cancer cell culture (pp. 237-245). Humana Press.
  23. Saravanan, B.C., Sreekumar, C., Bansal, G.C., Ray, D., Rao, J.R. and Mishra, A.K., 2003. A rapid MTT colorimetric assay to assess the proliferative index of two Indian strains of Theileria annulata. Veterinary parasitology, 113(3-4), pp.211-216. https://doi.org/10.1016/S0304-4017(03)00062-1
  24. Kim, S.S., Kim, M.J., Choi, Y.H., Kim, B.K., Kim, K.S., Park, K.J., Park, S.M., Lee, N.H. and Hyun, C.G., 2013. Down-regulation of tyrosinase, TRP-1, TRP-2 and MITF expressions by citrus press-cakes in murine B16 F10 melanoma. Asian Pacific Journal of Tropical Biomedicine, 3(8), pp.617-622. https://doi.org/10.1016/S2221-1691(13)60125-2
  25. Hunt, G., Todd, C., Cresswell, J.E. and Thody, A.J., 1994. Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. Journal of cell science, 107(1), pp.205-211. https://doi.org/10.1242/jcs.107.1.205