Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Development and characterization of hyaluronic acid-based orally disintegrating film containing vitamin D

히알루론산 기반 비타민 D 함유 구강용해필름의 제조 및 특성평가

  • Kang, Seo-Yeon (Department of Food and Nutrition, Sangmyung University) ;
  • An, Da-Yeon (Department of Food and Nutrition, Sangmyung University) ;
  • Han, Jung-Ah (Department of Food and Nutrition, Sangmyung University)
  • 강서연 (상명대학교 식품영양학과) ;
  • 안다연 (상명대학교 식품영양학과) ;
  • 한정아 (상명대학교 식품영양학과)
  • Received : 2022.03.06
  • Accepted : 2022.04.21
  • Published : 2022.06.30
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Abstract

An orally disintegrating film (ODF) based on hyaluronic acid (HA) containing vitamin D was developed. The vitamin D content in the ODF was set based on the adequate intake (AI) of vitamin D from 0 to 10 AI (0, 1, 4, 7, and 10AI). The control (0AI) had the highest thickness and showed the longest disintegration time among the samples. The moisture content of the ODFs was significantly lower in those with vitamin D compared to the control. As the amount of vitamin D increased, the water vapor permeability (WVP) of the ODFs decreased, and the opacity significantly increased. The tensile strength was higher in the films containing vitamin D compared to the control films. However, the elongation at the break showed no significant difference among the films. The vitamin D content in the film was reduced by 25.7-44.2% during processing compared to the amount that was originally added. Based on the above results, a new and convenient vitamin D delivery system, an ODF, could be successfully produced.

칼슘과 함께 뼈 건강에 필수적이나, 그 섭취량이 부족하여 문제가 되고 있는 비타민 D를 쉽게 보충할 수 있는 새로운 제형으로 천연 고분자 물질인 히알루론산을 기반으로 한 비타민 D 함유 구강용해필름(orally disintegrating film, ODF)을 개발하고 비타민 D 함량에 따른 필름의 특성을 분석하였다. 첨가량은 2020 한국인 영양소섭취기준의 비타민 D 하루 충분섭취량(400IU: 10 ㎍)을 기준으로 4, 7배, 그리고 상한섭취량인 10배로 설정하였다. 제조한 필름의 두께는 기반물질의 농도가 가장 높은 대조군이 가장 두꺼웠고, 비타민 D 첨가량에 따른 유의적 차이는 없었다(p<0.05). 비타민 D 첨가군간 필름의 수분함량의 차이는 없었으며, 첨가량이 많아질수록 투습도는 다소 감소하는 경향을 보여 소수성 물질인 비타민 D가 영향을 미친 것으로 보인다. 비타민 D의 함량이 높아질수록 필름의 명도는 10AI만 유의적으로 높았으며, 적색도는 감소하고 황색도는 증가하였다(p<0.05). 이와 같은 색도특성은 첨가한 비타민 D 시료 자체 색의 영향을 받은 것으로 보인다. 대조군과 비교하였을 때 비타민 D의 증가는 불투명도를 유의적으로 증가시켰으며(p<0.05), 7AI와 10AI에서 가장 높은 불투명도를 보였다. 다른 친수성 고분자 필름과 달리 본 연구에서 제조된 히알루론산 기반 필름은 가소제의 첨가 없이도 타 연구의 필름에 비교하여 높은 인장강도(84.40-106.6 MPa)(p<0.05)와, 비슷한 수준의 연신율(4.71-9.43%)(p>0.05)을 갖는 질감 특성을 보였다. HPLC/MS 분석을 통해 필름 내의 실제 비타민 D 함량을 분석한 결과, 제조 과정 중 비타민 D의 손실이 발견되었으며 이로 인해 목표섭취량(2020 한국인 영양소섭취기준 비타민 D 충분섭취량)을 충족하기 위해서는 제조 기준의 1.5-2배를 섭취하는 것이 적절할 것으로 보인다. 본 연구에서는 생체물질인 히알루론산을 기반으로 비타민 D를 쉽게 섭취할 수 있는 새로운 제형을 개발하였으며, 비타민 D 첨가로 인한 필름의 특성이 향상되는 결과를 이용하여 구강용해용 뿐 아니라 가식성 포장재 등 다양한 활용이 가능할 것으로 제안하는 바이다.

Keywords

Acknowledgement

이 성과는 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(NRF-2019R1A2C1002782).

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