The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of Two Unreported Sordariomycetes Fungi in Korea: Pestalotiopsis clavata and Botryotrichum iranicum

  • Seong-Keun Lim (School of Applied Biosciences, Kyungpook National University) ;
  • Leonid N. Ten (School of Applied Biosciences, Kyungpook National University) ;
  • Diane Avalos-Ruiz (School of Applied Biosciences, Kyungpook National University) ;
  • Jung-Joo Ryu (School of Applied Biosciences, Kyungpook National University) ;
  • In-Kyu Kang (Department of Horticultural Science, Kyungpook National University) ;
  • Seung-Yeol Lee (School of Applied Biosciences, Kyungpook National University) ;
  • Hee-Young Jung (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.06.10
  • Accepted : 2022.09.13
  • Published : 2022.09.30
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Abstract

Two fungal strains, designated KNUF-21-006 and KNUF-21-028, were isolated from soil samples collected from Gyeongbuk Province, Korea. The strain KNUF-21-006 was similar to other Pestalotiopsis species in terms of morphological characteristics, including whitish to pale brown mycelium, conidial shape, and size. The isolate had aerial hyphae that produced black fruiting bodies on the mycelium. The conidia were fusoid to ellipsoid, four-septate, and appendage-bearing. Phylogenetic analysis using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF), and β-tubulin (TUB) gene sequences confirmed that the closest relationship of the isolate at the species level was with Pestalotiopsis clavata. The strain KNUF-21-028 exhibits similar morphological characteristics to other Botryotrichum species, including white aerial mycelium with sulcate and irregular margins, conidial shape, and size. The conidia were globose, single, and hyaline. Upon molecular analysis-using the ITS region, large subunit (LSU) rRNA gene, and TUB gene sequences-the fungus was identified as Botryotrichum iranicum. This is the first record of these fungal species in Korea.

Keywords

Acknowledgement

This study was supported by the National Institute of Biological Resources, funded by the Ministry of Environment of the Republic of Korea, for the survey and discovery of indigenous Korean fungal species.

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