Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Bacillus SW1-1 coated diets on innate immunity and disease resistance of olive flounder Paralichthys olivaceus against Edwardsiella tarda infection

  • Kim, Min-Gi (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Gunathilaka, Buddi E. (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Sungho (Central Research Institute, Woo Gene B&G) ;
  • Kim, Youjeong (Central Research Institute, Woo Gene B&G) ;
  • Lee, Kyeong-Jun (Department of Marine Life Sciences, Jeju National University)
  • Received : 2021.10.01
  • Accepted : 2022.02.23
  • Published : 2022.04.30
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Abstract

Bacillus SW1-1 is a probiotic isolated from shrimp intestines. We investigated the effects of Bacillus SW1-1 coated diets on the growth, feed utilization, innate immunity, hematological parameters and resistance to Edwardsiella tarda in olive flounder (Paralichthys olivaceus). A commercial diet was used as the control (AP0) and two other diets were prepared by coating 0.25% (AP25) or 0.50% (AP50) probiotic powder which contains 1.0 × 107 CFU/g Bacillus SW1-1. Four replicate groups of olive flounder (153 ± 2 g) were fed one of the diets for 12 weeks. Growth performance and feed utilization of the fish were not significantly affected by the dietary Bacillus SW1-1. After the challenge with E. tarda, AP50 group showed significantly higher survival than AP0 and AP25 groups. Innate immunity and anti-oxidant capacity of the fish were not significantly affected after the feeding trial. However, after the E. tarda challenge, the innate immune parameters (immunoglobulin, lysozyme and anti-protease) were significantly improved in fish fed AP25 and AP50 diets compared to those in fish fed AP0 diet. After the challenge test, significantly lower glucose level was observed in AP50 group compared to AP0 group. These results indicate that dietary supplementation of Bacillus SW1-1 could increase the disease resistance of olive flounder against E. tarda infection. The optimum coating levels of Bacillus SW1-1 needs to be further elucidated.

Keywords

Acknowledgement

We would like to thank staffs in Marine Science Institute and graduate students in Aquaculture Feeds & Nutrition Laboratory for their help in samplings and analyses.

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