Fisheries and Aquatic Sciences

한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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CgGH insertion functional domain analysis in transgenic G1 and G2 and G3 mutiara catfish (Clarias gariepinus) broodstock

  • Buwono, Ibnu Dwi (Aquaculture Department, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran) ;
  • Grandiosa, Roffi (Aquaculture Department, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran) ;
  • Mulyani, Yuniar (Aquaculture Department, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran)
  • 투고 : 2021.06.22
  • 심사 : 2021.10.26
  • 발행 : 2022.01.30
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초록

Catfish is one of the most important freshwater fish farming commodities in Indonesia. Higher catfish production can be achieved by cultivating transgenic catfish carrying the growth hormone (GH) gene of African catfish (Clarias gariepinus GH, CgGH). This research focuses on analysis of the presence of the CgGH gene in transgenic G1, G2, and G3 mutiara catfish broodstock, as an indication of stable CgGH inheritance. CgGH gene was isolated using the RNeasy mini kit and RT-PCR. RT-PCR revealed amplicons measuring approximately 600 bp in transgenic G0, G1, G2, and G3 mutiara catfish. The CgGH consensus sequence similarities ranged from 93.76% to 97.06%, with four functional domain sites (somatotropin-1, somatotropin-2, four α-helix, N-glycosylation, four cysteine residues) of fish GH proteins. The functional domains of fish GH proteins are conserved in G1, G2, and G3 and indicate stable exogenous GH inheritance to produce transgenic catfish strains in each generation.

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과제정보

The authors are thankful to the Directorate of Research and Community Service of the Universitas Padjadjaran for support of research. The author would like to thank to the research team and research assistants (Rahayu Ramadhayanti and Ayuniar Puteri) thank you for the technical support during the research.

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