Clinical and Experimental Reproductive Medicine

  • 제49권4호
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  • Pages.270-276
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  • 2022
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Prolonged semen incubation alters the biological characteristics of human spermatozoa

  • Sayed Abbas Datli Beigi (Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Mohammad Ali Khalili (Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Ali Nabi (Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Mohammad Hosseini (Department of Anatomical Sciences, Shahid Sadoughi University of Medical Sciences) ;
  • Abolghasem Abbasi Sarcheshmeh (Department of Anatomical Sciences, Shahid Sadoughi University of Medical Sciences) ;
  • Mojdeh Sabour (Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences)
  • 투고 : 2022.04.18
  • 심사 : 2022.08.10
  • 발행 : 2022.12.31
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초록

Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37℃. Methods: Twenty-five normozoospermic semen samples were incubated at 37℃. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval. Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p<0.001). No significant changes were observed in DNA fragmentation at 1 hour compared to 0 hours (median [interquartile range]: 19.5 [4] vs. 19 [4]), as well as at 1.5 hours compared to 1 hour (20 [5]). However, a significant increase in DNA fragmentation was observed at 1.5 hours compared to 0 hours. The mitochondrial membrane potential decreased remarkably after 1 hour of incubation time. No significant differences were observed in the acrosome reaction or malonaldehyde levels at any time point (p=0.34 and p=0.98, respectively). Conclusion: The incubation of normozoospermic samples before use in assisted reproductive technology should be less than 1.5 hours to minimize the destructive effects of prolonged incubation time on general and specific sperm parameters.

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참고문헌

  1. Rowe PJ, Comhaire FH, Hargreave TB, Mahmoud AM. WHO manual for the standardized investigation and diagnosis of the infertile male. Cambridge: Cambridge university press; 2000.
  2. Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol 2015;13:37.
  3. Gnoth C, Godehardt E, Frank-Herrmann P, Friol K, Tigges J, Freundl G. Definition and prevalence of subfertility and infertility. Hum Reprod 2005;20:1144-7.
  4. Jensen CF, Khan O, Nagras ZG, Sonksen J, Fode M, Ostergren PB, et al. Male infertility problems of patients with strict sperm morphology between 5-14% may be missed with the current WHO guidelines. Scand J Urol 2018;52:427-31. https://doi.org/10.1080/21681805.2018.1548503
  5. Sunder M, Leslie SW. Semen analysis [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 [cited 2022 Sep 19]. Available from: https://pubmed.ncbi.nlm.nih.gov/33232039/.
  6. Nabi A, Khalili MA, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia 2014;46:374-9. https://doi.org/10.1111/and.12088
  7. van der Westerlaken L, Naaktgeboren N, Verburg H, Dieben S, Helmerhorst FM. Conventional in vitro fertilization versus intracytoplasmic sperm injection in patients with borderline semen: a randomized study using sibling oocytes. Fertil Steril 2006;85:395-400. https://doi.org/10.1016/j.fertnstert.2005.05.077
  8. Mangoli E, Khalili MA, Talebi AR, Ghasemi-Esmailabad S, Hosseini A. Is there any correlation between sperm parameters and chromatin quality with embryo morphokinetics in patients with male infertility? Andrologia 2018;50:e12997.
  9. Yang H, Li G, Jin H, Guo Y, Sun Y. The effect of sperm DNA fragmentation index on assisted reproductive technology outcomes and its relationship with semen parameters and lifestyle. Transl Androl Urol 2019;8:356-65. https://doi.org/10.21037/tau.2019.06.22
  10. Zhu XB, Chen Q, Fan WM, Niu ZH, Xu BF, Zhang AJ. Sperm DNA fragmentation in Chinese couples with unexplained recurrent pregnancy loss. Asian J Androl 2020;22:296-301. https://doi.org/10.4103/aja.aja_60_19
  11. Ritchie C, Ko EY. Oxidative stress in the pathophysiology of male infertility. Andrologia 2021;53:e13581.
  12. Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. Etiologies of sperm oxidative stress. Int J Reprod Biomed 2016;14:231-40. https://doi.org/10.29252/ijrm.14.4.231
  13. Talebi AR, Khalili MA, Vahidi S, Ghasemzadeh J, Tabibnejad N. Sperm chromatin condensation, DNA integrity, and apoptosis in men with spinal cord injury. J Spinal Cord Med 2013;36:140-6. https://doi.org/10.1179/2045772312Y.0000000055
  14. Bashiri Z, Amidi F, Amiri I, Zandieh Z, Maki CB, Mohammadi F, et al. Male factors: the role of sperm in preimplantation embryo quality. Reprod Sci 2021;28:1788-811. https://doi.org/10.1007/s43032-020-00334-z
  15. Amaral A, Lourenco B, Marques M, Ramalho-Santos J. Mitochondria functionality and sperm quality. Reproduction 2013;146:R163-74. https://doi.org/10.1530/REP-13-0178
  16. Ickowicz D, Finkelstein M, Breitbart H. Mechanism of sperm capacitation and the acrosome reaction: role of protein kinases. Asian J Androl 2012;14:816-21.
  17. El-Taieb MA, Ali MA, Nada EA. Oxidative stress and acrosomal morphology: a cause of infertility in patients with normal semen parameters. Middle East Fertil Soc J 2015;20:79-85. https://doi.org/10.1016/j.mefs.2014.05.003
  18. World Health Organization. WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organization; 2010.
  19. Anbari F, Halvaei I, Nabi A, Ghazali S, Khalili MA, Johansson L. The quality of sperm preparation medium affects the motility, viability, and DNA integrity of human spermatozoa. J Hum Reprod Sci 2016;9:254-8. https://doi.org/10.4103/0974-1208.197691
  20. Nabi A, Khalili MA, Fesahat F, Talebi A, Ghasemi-Esmailabad S. Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity. Cryobiology 2017;76:59-64. https://doi.org/10.1016/j.cryobiol.2017.04.008
  21. Esteves SC, Sharma RK, Thomas AJ Jr, Agarwal A. Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test. Int Braz J Urol 2007;33:364-76. https://doi.org/10.1590/S1677-55382007000300009
  22. Sivandzade F, Bhalerao A, Cucullo L. Analysis of the mitochondrial membrane potential using the cationic JC-1 dye as a sensitive fluorescent probe. Bio Protoc 2019;9:e3128.
  23. Karimi Zarchi M, Maleki B, Dehghani Ashkezari M, Motamed Zadeh L, Agha-Rahimi A. The effects of in vitro incubation of asthenoteratozoospermic semen after density gradient centrifugation at room temperature and 37℃ on sperm parameters, chromatin quality and DNA fragmentation in a short time period. J Reprod Infertil 2020;21:275-82.
  24. Thijssen A, Klerkx E, Huyser C, Bosmans E, Campo R, Ombelet W. Influence of temperature and sperm preparation on the quality of spermatozoa. Reprod Biomed Online 2014;28:436-42. https://doi.org/10.1016/j.rbmo.2013.12.005
  25. Ouitrakul S, Sukprasert M, Treetampinich C, Choktanasiri W, Vallibhakara SA, Satirapod C. The effect of different timing after ejaculation on sperm motility and viability in semen analysis at room temperature. J Med Assoc Thai 2018;101:26- 32.
  26. Nematollahi S, Mehdizadeh M, Hosseini S, Kashanian M, Amjadi FS, Salehi M. DNA integrity and methylation changes of mouse spermatozoa following prolonged incubation. Andrologia 2019;51:e13276.
  27. Peer S, Eltes F, Berkovitz A, Yehuda R, Itsykson P, Bartoov B. Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37℃? Fertil Steril 2007;88:1589-94. https://doi.org/10.1016/j.fertnstert.2007.01.069
  28. Ahmed I, Abdelateef S, Laqqan M, Amor H, Abdel-Lah MA, Hammadeh ME. Influence of extended incubation time on human sperm chromatin condensation, sperm DNA strand breaks and their effect on fertilisation rate. Andrologia 2018;50:e12960.
  29. Alvarez Sedo C, Bilinski M, Lorenzi D, Uriondo H, Noblia F, Longobucco V, et al. Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects. JBRA Assist Reprod 2017;21:343-50.
  30. Zhu Z, Kawai T, Umehara T, Hoque SA, Zeng W, Shimada M. Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria. Free Radic Biol Med 2019;141:159-71. https://doi.org/10.1016/j.freeradbiomed.2019.06.018
  31. Pujol A, Garcia D, Obradors A, Rodriguez A, Vassena R. Is there a relation between the time to ICSI and the reproductive outcomes? Hum Reprod 2018;33:797-806. https://doi.org/10.1093/humrep/dey067