대한한의학방제학회지 (Herbal Formula Science)

  • 제30권2호
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  • Pages.67-83
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  • 2022
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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DOI QR코드

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비기환, 대칠기탕 및 목향빈랑환 열수 추출물에 의한 인간 간세포암종 HepG2 세포의 세포사멸 유도에 미치는 자가포식의 역할

The Role of Autophagy on the Induction of Apoptosis by Water Extracts of Bigihwan, Daechilgitang and Mokwhyangbinranghwan in HepG2 Human Hepatocellular Carcinoma Cells

  • 박상은 (동의대학교 한의과대학 간계내과학교실) ;
  • 홍수현 (동의대학교 한의과대학 생화학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실)
  • Park, Sang Eun (Department of Korean Internal Medicine, College of Korean Medicine, Dong-eui University) ;
  • Hong, Su Hyun (Department of Biochemistry, College of Korean Medicine, Dong-eui University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Korean Medicine, Dong-eui University)
  • 투고 : 2022.04.14
  • 심사 : 2022.05.16
  • 발행 : 2022.05.31
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초록

Objectives : In this study, the anticancer activity of water extracts of three herbal medicine formulas, Bigihwan (BGH), Daechilgitang (DCGT) and Mokwhyangbinranghwan (MHBRH) listed in Donguibogam, was evaluated in HepG2 cells, a human hepatocellular carcinoma cell line. Methods : We investigated whether the cell viability of HepG2 cells was inhibited by the treatment of water extracts of three prescriptions, and whether their viability inhibitory effect was related to the induction of apoptosis. In addition, the role of autophagy on the induction of apoptosis by the treatment of these extracts was investigated. Results : The anticancer activity of the three water extracts on HepG2 cells was due to induction of apoptosis, not necrosis. Among them, BGH activated the caspase-dependent intrinsic apoptosis pathway associated with mitochondrial dysfunction. However, autophagy was induced more than 2-fold in DCGT-treated HepG2 cells, and the anticancer activity of DCGT was enhanced 1.5-fold in the presence of an autophagy inhibitor, but was attenuated in BGH and MHBRH-treated cells. Conclusion : The results of this study indicate that DCGT-induced autophagy was involved in the inhibition of apoptosis, whereas autophagy by BGH and MHBRH was related to induction of apoptosis.

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