Journal of the Korean Society of Manufacturing Process Engineers (한국기계가공학회지)

The Korean Society of Manufacturing Process Engineers (한국기계가공학회)
권호 표지
DOI QR코드

DOI QR Code

Fabrication of Microfibrous Structures with Rolled-Up Forms using a Bilayer Self-Assembly Process

이중층 자가조립 공정을 활용한 롤형태의 생체의료용 마이크로섬유 구조체 제작

  • Kim, Yeong-Seo (School of Mechanical Engineering, Pusan National University) ;
  • Park, Suk-Hee (School of Mechanical Engineering, Pusan National University)
  • 김영서 (부산대학교 기계공학부) ;
  • 박석희 (부산대학교 기계공학부)
  • Received : 2021.12.15
  • Accepted : 2022.01.04
  • Published : 2022.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Numerous fabrication techniques have been used to mimic cylindrical natural tissues, such as blood vessels, tendons, ligaments, and skeletal muscles. However, most processes have limitations in achieving the biomimetic properties of multilayered and porous architectures. In this study, to embrace both features, a novel self-assembly method was proposed using electrospun microfibrous sheets. A bilayer microfibrous structure, comprising two sheets with different internal stresses, was fabricated by electrospinning a polycaprolactone (PCL) sheet on a uniaxially stretched thermoplastic polyurethane (TPU) sheet. Then, by removing the stretching tension, the sheet was rolled into a hollow cylindrical structure with a specific internal diameter. The internal diameter could be quantitatively controlled by adjusting the thickness of the PCL sheet against that of the TPU sheet. Through this self-assembly method, biomimetic cylindrical structures with multilayer and porous features can be manufactured in a stable and controllable manner. Therefore, the resulting structures may be applied to various tissue engineering scaffolds, especially vascular and connective tissues.

Keywords

Acknowledgement

본 연구는 2020년도 정부(과학기술정보통신부)의 재원으로 정보통신기획평가원의 지원(No.2020-0-00228, 맞춤형 자유형상 인조혈관 설계 소프트웨어 및 3D프린팅 기반 제조공정 기술 개발)과 2020학년도 부산대학교 BK21 FOUR 대학원혁신지원사업 지원으로 이루어졌음.

References

  1. Park, J.-H., Byon, S.-M., "Drawing Process with Rotational Die for Forming Grooves in a Tube," Journal of the Korean Society of Manufacturing Process Engineers Vol. 17, No. 4, pp. 123-129, 2018.
  2. Chen, C.-H., Takita, K., Ishiguro, S., Honda, S., and Awaji, H., "Fabrication on porous alumina tube by centrifugal molding," Journal of the European ceramic society, Vol. 25, No. 14, pp. 3257-3264, 2005. https://doi.org/10.1016/j.jeurceramsoc.2004.08.019
  3. Lee, J.-E., Park, S.-J., Yoon, Y., Son, Y., and Park, S.-H., "Fabrication of 3D freeform porous tubular constructs with mechanical flexibility mimicking that of soft vascular tissue," Journal of the mechanical behavior of biomedical materials, Vol. 91, pp. 193-201, 2019. https://doi.org/10.1016/j.jmbbm.2018.12.020
  4. Kim, S.-E., Hyeon, Y.-T., Yun, H.-S., "Tissue engineering scaffold technology for bone regeneration," mechanic and materials, Vol. 18, No. 4, pp. 85-91, 2006.
  5. Dehghani, F. and Annabi, N., "Engineering porous scaffolds using gas-based techniques," Current opinion in biotechnology, Vol. 22, No. 5, pp. 661-666, 2011. https://doi.org/10.1016/j.copbio.2011.04.005
  6. Inoguchi, H., Kwon, I.-K., Inoue, E., Takamizawa, K., Maehara, Y., and Matsuda, T., "Mechanical responses of a compliant electrospun poly (L-lactide-co-ε-caprolactone) small-diameter vascular graft," Biomaterials, Vol. 27, No. 8, pp. 1470-1478, 2006. https://doi.org/10.1016/j.biomaterials.2005.08.029
  7. Park, S.-H., Go, U.-H., and Sin, H.-J., "Method of manufacturing electrospinning nanofiber scafflods for tissue engineering," Journal of the KSME, Vol. 55, No. 11, pp. 34-39, 2015.
  8. Yin, A., Zhang, K., McClure, M., Huang, C., Wu, J., Fang, J., Mo, X., Bowlin, G., Al-Deyab, S.-S., El-Newehy, M., "Electrospinning collagen/ chitosan/ poly (L lactic acid-co-e-caprolactone) to form a vascular graft: Mechanical and biological characterization," Journal of biomedical materials research Part A, Vol. 101, No. 5, pp. 1292-1301, 2013.
  9. Li, Y., Jiang, K., Feng, J., Liu, J., Huang, R., Chen, Z., Yang, J., Dai, Z., Chen, Y., Wang, N., Zang, W., Zheng, W., Yang, G. and Jiang, X., "Construction of Small Diameter Vascular Graft by Shape-Memory and Self-Rolling Bacterial Cellulose Membrane," Advanced healthcare materials, Vol. 6, No. 11, p. 1601343, 2017. https://doi.org/10.1002/adhm.201601343
  10. Fontoura, J.-C., Viezzer, C., dos Santos, F.-G., Ligabue, R.-A., Weinlich, R., Puga, R.-D., Antonow, D., Severino, P., Bonorino, C., "Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance," Materials Science and Engineering: C, Vol. 107, pp. 110264, 2020. https://doi.org/10.1016/j.msec.2019.110264
  11. Li, Y., Yuan, B., Ji, H., Han, D., Chen, S., Tian, F., Jiang, X., "A method for patterning multiple types of cells by using electrochemical desorption of self-assembled monolayers within microfluidic channels," Angewandte Chemie International Edition, Vol. 46, No. 7, pp. 1094-1096, 2007. https://doi.org/10.1002/anie.200603844
  12. Park, N.-J., Choi, S.-B., Cheong, C.-C.. "Vibration and Position Tracking Control of a Smart Structure Using SMA Actuators" Journal of the Korean Society for Precision Engineering Vol. 13, No. 8, pp. 155-163, 1996.
  13. Park S.-S., Bae, D.-S., Bae, D.-H.. "Development of new bimetal material for home appliances by using the rolling process" Proceedings of the Korean Society for Technology of Plasticity Conference, pp. 389-393, 2007.
  14. Yuan, B., Jin, Y., SUn, Y., Wang, D., Sun, J., Wang, Z., Zhang, W., Jiang, X., "A strategy for depositing different types of cells in three dimensions to mimic tubular structures in tissues," Advanced Materials, Vol. 24, No. 7, pp. 890-896, 2012. https://doi.org/10.1002/adma.201104589
  15. Mercado-Pagan, A.-E., Kang, Y., Findlay, M.-W., Yang, Y., "Development and evaluation of elastomeric hollow fiber membranes as small diameter vascular graft substitutes," Materials Science and Engineering: C, Vol. 49, pp. 541-548, 2015. https://doi.org/10.1016/j.msec.2015.01.051
  16. Li, Q., Mu, L., Zhang, F., Mo, Z., Jin, C., and Qi, W., "Manufacture and property research of heparin grafted electrospinning PCU artificial vascular scaffolds," Materials Science and Engineering: C, Vol. 78, pp. 854-861,
  17. Chen, M., Patra, P.-K., Warner, S.-B., and Bhowmick, S., "Role of fiber diameter in adhesion and proliferation of NIH 3T3 fibroblast on electrospun polycaprolactone scaffolds," Tissue engineering, Vol. 13, No. 3, pp. 579-587, 2007. https://doi.org/10.1089/ten.2006.0205
  18. Christopherson, G.-T., Song, H., and Mao H.-Q., "The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation," Biomaterials, Vol. 30, No. 4, pp. 556-564, 2009. https://doi.org/10.1016/j.biomaterials.2008.10.004
  19. Gao, J., Guo, H., Tian, S., Qiao, Y., Han, J., Li, Y., Wang, L., "Preparation and mechanical performance of small-diameter PHBHHx vascular graft by electrospinning," International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 68, No. 10, pp. 575-581, 2019. https://doi.org/10.1080/00914037.2018.1473865
  20. Johnson, R., Ding, Y., Nagiah N., Monnet E., Tan, W., "Coaxially-structured fibres with tailored material properties for vascular graft implant," Materials Science and Engineering: C, Vol. 97, pp. 1-11, 2019. https://doi.org/10.1016/0025-5416(88)90003-1
  21. Kamal, T., Shin, T.-J., Park, S.-Y., "Uniaxial tensile deformation of poly ( -caprolactone) studied with ε SAXS and WAXS techniques using synchrotron radiation," Macromolecules, Vol. 45, No. 21, pp. 8752-8759, 2012. https://doi.org/10.1021/ma301714f
  22. Badhe, R.-V., Bijukumar, D., Chejara, D.-R., Mabrouk, M., Choonara, Y.-E., Kumar, P., du Toit, L.-C., Kondiah, P.-P.-D., Pillay, V., "A composite chitosan- gelatin bi-layered, biomimetic macroporous scaffold for blood vessel tissue engineering," Carbohydrate polymers, Vol. 157, pp. 1215-1225, 2017. https://doi.org/10.1016/j.carbpol.2016.09.095
  23. Jones, R.-S., Nawana, N.-S., Pearcy, M.-J., Learmonth, D.-J.-A., Bickerstaff, D.-R., Costi, J.-J., Paterson, R.-S., "Mechanical properties of the human anterior cruciate ligament," Clinical Biomechanics, Vol. 10, No. 7, pp. 339-344, 1995. https://doi.org/10.1016/0268-0033(95)98193-X