The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Enhancement of Disease Control Efficacy of Chemical Fungicides Combined with Plant Resistance Inducer 2,3-Butanediol against Turfgrass Fungal Diseases

  • Duraisamy, Kalaiselvi (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Ha, Areum (Plant Healthcare Research Institute, JAN153 Biotech Incorporated) ;
  • Kim, Jongmun (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Park, Ae Ran (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Bora (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Song, Chan Woo (Research and Development Center, GS Caltex Corporation) ;
  • Song, Hyohak (Research and Development Center, GS Caltex Corporation) ;
  • Kim, Jin-Cheol (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 투고 : 2022.02.17
  • 심사 : 2022.03.17
  • 발행 : 2022.06.01
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초록

Turfgrass, the most widely grown ornamental crop, is severely affected by fungal pathogens including Sclerotinia homoeocarpa, Rhizoctonia solani, and Magnaporthe poae. At present, turfgrass fungal disease management predominantly relies on synthetic fungicide treatments. However, the extensive application of fungicides to the soil increases residual detection frequency, raising concerns for the environment and human health. The bacterial volatile compound, 2,3-butanediol (BDO), was found to induce plant resistance. In this study, we evaluated the disease control efficacy of a combination of stereoisomers of 2,3-BDO and commercial fungicides against turfgrass fungal diseases in both growth room and fields. In the growth room experiment, the combination of 0.9% 2R,3R-BDO (levo) soluble liquid (SL) formulation and 9% 2R,3S-BDO (meso) SL with half concentration of fungicides significantly increased the disease control efficacy against dollar spot and summer patch disease when compared to the half concentration of fungicide alone. In field experiments, the disease control efficiency of levo 0.9% and meso 9% SL, in combination with a fungicide, was confirmed against dollar spot and large patch disease. Additionally, the induction of defense-related genes involved in the salicylic acid and jasmonic acid/ethylene signaling pathways and reactive oxygen species detoxification-related genes under Clarireedia sp. infection was confirmed with levo 0.9% and meso 9% SL treatment in creeping bentgrass. Our findings suggest that 2,3-BDO isomer formulations can be combined with chemical fungicides as a new integrated tool to control Clarireedia sp. infection in turfgrass, thereby reducing the use of chemical fungicides.

키워드

과제정보

This research was supported by GS Caltex Corporation, Republic of Korea.

참고문헌

  1. Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55:373-399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
  2. Backer, R., Naidoo, S. and van den Berg, N. 2019. The nonexpressor of pathogenesis-related genes 1 (NPR1) and related family: mechanistic insights in plant disease resistance. Front. Plant Sci. 10:102. https://doi.org/10.3389/fpls.2019.00102
  3. Barrett, E. L., Collins, E. B., Hall, B. J. and Matoi, S. H. 1983. Production of 2,3-butylene glycol from whey by Klebsiella pneumoniae and Enterobacter aerogenes. J. Dairy Sci. 66:2507-2514. https://doi.org/10.3168/jds.s0022-0302(83)82119-5
  4. Beard, J. B. and Green, R. L. 1994. The role of turfgrasses in environmental protection and their benefits to humans. J. Environ. Qual. 23:452-460. https://doi.org/10.2134/jeq1994.00472425002300030007x
  5. Benelli, J. J., Horvath, B. J., Womac, A. R., Ownley, B. H., Windham, A. S. and Sorochan, J. C. 2018. Large patch (Rhizoctonia solani AG 2-2LP) severity on Japanese lawngrass (Zoysia japonica) influenced by fungicide and application target site. Crop Prot. 106:23-28. https://doi.org/10.1016/j.cropro.2017.12.003
  6. Biczak, R. 2016. Quaternary ammonium salts with tetrafluoroborate anion: phytotoxicity and oxidative stress in terrestrial plants. J. Hazard. Mater. 304:173-185. https://doi.org/10.1016/j.jhazmat.2015.10.055
  7. Chung, J.-h., Song, G. C. and Ryu, C.-M. 2016. Sweet scents from good bacteria: case studies on bacterial volatile compounds for plant growth and immunity. Plant Mol. Biol. 90:677-687. https://doi.org/10.1007/s11103-015-0344-8
  8. Cortes-Barco, A. M., Hsiang, T. and Goodwin, P. H. 2010. Induced systemic resistance against three foliar diseases of Agrostis stolonifera by (2R,3R)-Butanediol or an isoparaffin mixture. Ann. Appl. Biol. 157:179-189. https://doi.org/10.1111/j.1744-7348.2010.00417.x
  9. D'Alessandro, M., Erb, M., Ton, J., Brandenburg, A., Karlen, D., Zopfi, J. and Turlings, T. C. J. 2014. Volatiles produced by soil-borne endophytic bacteria increase plant pathogen resistance and affect tritrophic interactions. Plant Cell Environ. 37:813-826. https://doi.org/10.1111/pce.12220
  10. Damalas, C. A. and Eleftherohorinos, I. G. 2011. Pesticide exposure, safety issues, and risk assessment indicators. Int. J. Environ. Res. Public Health 8:1402-1419. https://doi.org/10.3390/ijerph8051402
  11. Garg, S. K. and Jain, A. 1995. Fermentative production of 2,3-butanediol: a review. Bioresour. Technol. 51:103-109. https://doi.org/10.1016/0960-8524(94)00136-O
  12. Gonzalez-Bosch, C. 2018. Priming plant resistance by activation of redox-sensitive genes. Free Radic. Biol. Med. 122:171-180. https://doi.org/10.1016/j.freeradbiomed.2017.12.028
  13. Han, S. H., Lee, S. J., Moon, J. H., Park, K. H., Yang, K. Y., Cho, B. H., Kim, K. Y., Kim, Y. W., Lee, M. C., Anderson, A. J. and Kim, Y. C. 2006. GacS-dependent production of 2R, 3R-butanediol by Pseudomonas chlororaphis O6 is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv. tabaci in tobacco. Mol. Plant-Microbe Interact. 19:924-930. https://doi.org/10.1094/mpmi-19-0924
  14. Hasanuzzaman, M., Bhuyan, M. H. M. B., Anee, T. I., Parvin, K., Nahar, K., Mahmud, J. A. and Fujita, M. 2019. Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants (Basel) 8:384. https://doi.org/10.3390/antiox8090384
  15. Kim, D., Yoon, J., Yoo, J., Kim, S.-J. and Yang, J. E. 2014. Status and management strategy of pesticide use in golf courses in Korea. J. Appl. Biol. Chem. 57:267-277 (in Korean). https://doi.org/10.3839/jabc.2014.043
  16. Kobayashi, D. Y., Guglielmoni, M. and Clarke, B. B. 1995. Isolation of the chitinolytic bacteria Xanthomonas maltophilia and Serratia marcescens as biological control agents for summer patch disease of turfgrass. Soil Biol. Biochem. 27:1479-1487. https://doi.org/10.1016/0038-0717(95)00062-J
  17. Kong, H. G., Shin, T. S., Kim, T. H. and Ryu, C.-M. 2018. Stereoisomers of the bacterial volatile compound 2,3-butanediol differently elicit systemic defense responses of pepper against multiple viruses in the field. Front. Plant Sci. 9:90. https://doi.org/10.3389/fpls.2018.00090
  18. Liu, Y., Lonappan, L., Brar, S. K. and Yang, S. 2018. Impact of biochar amendment in agricultural soils on the sorption, desorption, and degradation of pesticides: a review. Sci. Total Environ. 645:60-70. https://doi.org/10.1016/j.scitotenv.2018.07.099
  19. Mofidnakhaei, M., Abdossi, V., Dehestani, A., Pirdashti, H. and Babaeizad, V. 2016. Potassium phosphite affects growth, antioxidant enzymes activity and alleviates disease damage in cucumber plants inoculated with Pythium ultimum. Arch. Phytopathol. Plant Prot. 49:207-221. https://doi.org/10.1080/03235408.2016.1180924
  20. Mohammadi, M. A., Cheng, Y., Aslam, M., Jakada, B. H., Wai, M. H., Ye, K., He, X., Luo, T., Ye, L., Dong, C., Hu, B., Priyadarshani, S. V. G. N., Wang-Pruski, G. and Qin, Y. 2021. ROS and oxidative response systems in plants under biotic and abiotic stresses: revisiting the crucial role of phosphite triggered plants defense response. Front. Microbiol. 12:631318. https://doi.org/10.3389/fmicb.2021.631318
  21. Ogura, A. P., Lima, J. Z., Marques, J. P., Massaro Sousa, L., Rodrigues, V. G. S. and Espindola, E. L. G. 2021. A review of pesticides sorption in biochar from maize, rice, and wheat residues: current status and challenges for soil application. J. Environ. Manage. 300:113753. https://doi.org/10.1016/j.jenvman.2021.113753
  22. Pajerowska-Mukhtar, K. M., Emerine, D. K. and Mukhtar, M. S. 2013. Tell me more: roles of NPRs in plant immunity. Trends Plant Sci. 18:402-411. https://doi.org/10.1016/j.tplants.2013.04.004
  23. Park, K. Y., Seo, S. Y., Oh, B.-R., Seo, J.-W. and Kim, Y. J. 2018. 2,3-Butanediol induces systemic acquired resistance in the plant immune response. J. Plant Biol. 61:424-434. https://doi.org/10.1007/s12374-018-0421-z
  24. Pieterse, C. M., Leon-Reyes, A., Van der Ent, S. and Van Wees, S. C. M. 2009. Networking by small-molecule hormones in plant immunity. Nat. Chem. Biol. 5:308-316. https://doi.org/10.1038/nchembio.164
  25. Rahman, A., Uddin, W. and Wenner, N. G. 2015. Induced systemic resistance responses in perennial ryegrass against Magnaporthe oryzae elicited by semi-purified surfactin lipopeptides and live cells of Bacillus amyloliquefaciens. Mol. Plant Pathol. 16:546-558. https://doi.org/10.1111/mpp.12209
  26. Ryu, C.-M., Farag, M. A., Hu, C.-H., Reddy, M. S., Kloepper, J. W. and Pare, P. W. 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol. 134:1017-1026. https://doi.org/10.1104/pp.103.026583
  27. Ryu, C.-M., Farag, M. A., Hu, C.-H., Reddy, M. S., Wei, H.-X., Pare, P. W. and Kloepper, J. W. 2003. Bacterial volatiles promote growth in Arabidopsis. Proc. Natl Acad. Sci. U. S. A. 100:4927-4932. https://doi.org/10.1073/pnas.0730845100
  28. Sharma, S., Chen, C., Navathe, S., Chand, R. and Pandey, S. P. 2019. A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection. Sci. Rep. 9:4054. https://doi.org/10.1038/s41598-019-40930-x
  29. Shi, Y., Liu, X., Fang, Y., Tian, Q., Jiang, H. and Ma, H. 2018. 2, 3-Butanediol activated disease-resistance of creeping bentgrass by inducing phytohormone and antioxidant responses. Plant Physiol. Biochem. 129:244-250. https://doi.org/10.1016/j.plaphy.2018.06.010
  30. Stackhouse, T., Martinez-Espinoza, A. D. and Ali, M. E. 2020. Turfgrass disease diagnosis: past, present, and future. Plants (Basel) 9:1544.
  31. Tyagi, S., Lee, K.-J., Shukla, P. and Chae, J.-C. 2020. Dimethyl disulfide exerts antifungal activity against Sclerotinia minor by damaging its membrane and induces systemic resistance in host plants. Sci. Rep. 10:6547. https://doi.org/10.1038/s41598-020-63382-0
  32. Urooj, F., Farhat, H., Tariq, A., Moin, S., Sohail, N., Sultana, V., Hameedi, S. F., Shams, Z. I. and Ehteshamul-Haque, S. 2021. Role of endophytic Penicillium species and Pseudomonas monteilii in inducing the systemic resistance in okra against root rotting fungi and their effect on some physiochemical properties of okra fruit. J. Appl. Microbiol. 130:604-616. https://doi.org/10.1111/jam.14894
  33. Wightwick, A., Walters, R., Allinson, G., Reichman, S. and Menzies, N. 2010. Environmental risks of fungicides used in horticultural production systems. In: Disease decision support systems: their impact on disease management and durability of fungicide effectiveness: fungicides, eds. by O. Carisse, D.-M. Tremblay, T. Jobin and A. S. Walker, pp. 273-304. InTech, Rijeka, Croatia.
  34. Yi, H.-S., Ahn, Y.-R., Song, G. C., Ghim, S.-Y., Lee, S., Lee, G. and Ryu, C.-M. 2016. Impact of a bacterial volatile 2,3-butanediol on Bacillus subtilis Rhizosphere Robustness. Front. Microbiol. 7:993. https://doi.org/10.3389/fmich.2016.00993
  35. Zhou, M., Hu, Q., Li, Z., Li, D., Chen, C.-F. and Luo, H. 2011. Expression of a novel antimicrobial peptide Penaeidin4-1 in creeping bentgrass (Agrostis stolonifera L.) enhances plant fungal disease resistance. PLoS ONE 6:e24677. https://doi.org/10.1371/journal.pone.0024677