Mycobiology

The Korean Society of Mycology (한국균학회)
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Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

  • Wang, Laiyou (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Guo, Shuxian (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Zeng, Bo (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Wang, Shanshan (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Chen, Yan (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Cheng, Shuang (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Liu, Bingbing (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Wang, Chunyan (School of Biological and Chemical Engineering, Nanyang Institute of Technology) ;
  • Wang, Yu (College of Biological Science and Engineering, Jiangxi Agricultural University) ;
  • Meng, Qingshan (State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University)
  • Received : 2021.11.03
  • Accepted : 2022.02.02
  • Published : 2022.02.28
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Abstract

The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

Keywords

Acknowledgement

This work was supported by Basic and Frontier Research Project of Nanyang city (JCQY010), Interdisciplinary Research Project of Nanyang Institute of Technology (JC20191205), Opening Foundation of Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology (HIMFT20200101 and HIMFT20200204), National Natural Science Foundation of China (31800001), Key Scientific Research Project of Colleges and Universities in Henan Province (20A180019) and Key Technologies R&D Program of Nanyang city (KJGG079).

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