Mycobiology

The Korean Society of Mycology (한국균학회)
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Diaporthe eleutharrhenae sp. nov. Associated with a Critically Endangered Liana in China

  • Song, Shijie (Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences) ;
  • Landrein, Sven (Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences)
  • Received : 2021.12.22
  • Accepted : 2022.03.22
  • Published : 2022.04.30
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Abstract

A new fungus isolated from the leaves of Eleutharrhena macrocarpa in southern Yunnan, China is described using morphological and molecular evidence. Phylogenetic trees based on the combined nuclear ribosomal DNA internal transcribed spacer (ITS), translation elongation factor-1α (TEF1), and β-tubulin gene (TUB2) sequences showed that Diaporthe eleutharrhenae sp. nov. is sister to Diaporthe chinensis N.I. de Silva, Lumyong & K.D. Hyde and morphologically differs in shorter alpha conidia (5-8.5× 1.5-2 ㎛) and the presence of beta conidia. This study also resolves a nomenclatural problem, as two taxa were published using the same name. To avoid confusion, the unrelated D. chinensis H. Dong, J. W. Xia & X. G. Zhang is here renamed as D. dongii (H. Dong, J. W. Xia & X. G. Zhang) S. J. Song & Landrein, sp. nov. in honor of the author that described this species. Study and description of fungi associated with threatened tropical species could help to understand their ecology as well as the potential spread of fungi onto cultivated crop species.

Keywords

Acknowledgement

The authors thank Jo Osborne for manuscript review and editing, and thank to Pang ZhiQiang and Hao Chunhui and Shao ShiCheng for providing suggestions on conducting this study.

References

  1. Qian LS, Chen JH, Deng T, et al. Plant diversity in Yunnan: current status and future directions. Plant Divers. 2020;42(4):281-291. https://doi.org/10.1016/j.pld.2020.07.006
  2. Zhu H, Yan LC. 2012. Native seed plants in Xishuangbanna of Yunnan. Beijing, China: Science Press.
  3. Hou ZQ, Zhou D, Hou SN, et al. Present situation of Eleutharrhena macrocarpa in China. Plant Divers Resour. 2015;37(5):640-646.
  4. Muharini R, Enawaty E. Cytotoxic activity of stem of Pycnarrhena cauliflora through apoptosis induction on human breast cancer cell line T47D. Pharm Sci Res. 2019;6(3):5.
  5. Mishra A, Gond SK, Kumar A, et al. Season and tissue type affect fungal endophyte communities of the Indian medicinal plant Tinospora cordifolia more strongly than geographic location. Microb Ecol. 2012;64(2):388-398. https://doi.org/10.1007/s00248-012-0029-7
  6. Tanda S. Two new species of powdery mildew fungi from Japan. Mycoscience. 1994;35(1):49-52. https://doi.org/10.1007/BF02268527
  7. Yang Q, Fan XL, Guarnaccia V, et al. High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described. MycoKeys. 2018;39:97-149. https://doi.org/10.3897/mycokeys.39.26914
  8. Gao YH, Liu F, Duan WJ, et al. Diaporthe is paraphyletic. Ima Fungus. 2017;8(1):153-187. https://doi.org/10.5598/imafungus.2017.08.01.11
  9. Gomes RR, Glienke C, Videira SIR, et al. Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia. 2013;31:1-41. https://doi.org/10.3767/003158513X666844
  10. Sun XD, Cai XL, Pang QQ, et al. 2021. First report of Diaporthe longicolla causing leaf spot on Kalanchoe pinnata in China. Plant Dis. 2021; 105(11):3739.
  11. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocol. 1990;18(1): 315-322.
  12. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia. 1999;91(3):553-556. https://doi.org/10.2307/3761358
  13. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  14. De Silva NI, Maharachchikumbura SSN, Thambugala KM, et al. Morpho-molecular taxonomic studies reveal a high number of endophytic fungi from Magnolia candolli and M. garrettii in China and Thailand. Mycosphere. 2021;12(1): 163-237. https://doi.org/10.5943/mycosphere/12/1/3
  15. Dong H, Mu TC, Zhang Z, et al. Molecular phylogenetic analysis reveals two new species of Diaporthe from Yunnan province, southwestern China. Mycosystema. 2021;40:436-446.
  16. Zhang D, Gao FL, Jakovlic I, et al. PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Mol Ecol Resour. 2020;20(1):348-355. https://doi.org/10.1111/1755-0998.13096
  17. Kalyaanamoorthy S, Minh BQ, Wong TKF, et al. Model finder: fast model selection for accurate phylogenetic estimates. Nat Methods. 2017;14(6): 587-589. https://doi.org/10.1038/nmeth.4285
  18. Ronquist F, Teslenko M, Van Der Mark P, et al. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012;61(3):539-542. https://doi.org/10.1093/sysbio/sys029
  19. Minh BQ, Schmidt HA, Chernomor O, et al. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Mol Biol Evol. 2020;37(5):1530-1534. https://doi.org/10.1093/molbev/msaa015
  20. Udayanga D, Liua X, Mckenzie EH, et al. Multilocus phylogeny reveals three new species of Diaporthe from Thailand. Cryptog Mycol. 2012; 33(3):295-309. https://doi.org/10.7872/crym.v33.iss3.2012.295
  21. Huang F, Udayanga D, Wang X, et al. Endophytic Diaporthe associated with citrus: a phylogenetic reassessment with seven new species from China. Fungal Biol. 2015;119(5):331-347. https://doi.org/10.1016/j.funbio.2015.02.006
  22. Turland NJ, Wiersema JH, Barrie FR, et al. 2018. International code of nomenclature for algae, fungi, and plants (Shenzhen code) adopted by the nineteenth international botanical congress Shenzhen, China. Glashutten, Germany: Koeltz Botanical Books.