Mycobiology

The Korean Society of Mycology (한국균학회)
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Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum

  • Elsaba, Yasmin M. (Botany and Microbiology Department, Faculty of Science, Helwan University) ;
  • Boroujerdi, Arezue (Chemistry Department, Claflin University) ;
  • Abdelsalam, Asmaa (Botany and Microbiology Department, Faculty of Science, Helwan University)
  • Received : 2021.06.21
  • Accepted : 2022.03.26
  • Published : 2022.04.30
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Abstract

The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new Ceratorhiza hydrophila strain isolated from the submerged aquatic plant Myriophyllum spicatum. Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as Ceratorhiza hydrophila isolate EG19 and the fungus ID is MK387081. The enzyme analyses showed its ability to produce protease and cellulase enzymes. According to the CSLI standard, the ethyl acetate extract of C. hydrophila showed intermediate antibacterial activity against Streptococcus pneumonia, Micrococcus luteus, and Staphylococcus aureus. Metabolic profiling has been carried out using 700 MHz NMR spectroscopy. Based on the 1H and 1H-13C heteronuclear single quantum coherence (HSQC) NMR data and NMR databases, 23 compounds have been identified. The identified metabolites include 31% amino acids, 9% sugars, 9% amines, 4% sugar alcohols, and 4% alkaloids. This is the first report for the metabolic characterization of C. hydrophila, which gave preliminary information about the fungus. It is expected that our findings not only will pave the way to other perspectives in enormous applications using C. hydrophila as a new promising source of antimicrobial agents and essential metabolites, but also it will be valuable in the classification and chemotaxonomy of the species.

Keywords

Acknowledgement

The authors are very grateful to SC-INBRE (2 P20 GM103499) and NSF HBCU-UP (HRD-1332516) for providing NMR facilities. The authors are also grateful to Prof. Dr Tarek Galal, Professor of Plant Taxonomy, Botany and Microbiology Dept. Helwan University for helping in authenticating the plant material.

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