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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Endoplasmin regulates differentiation of tonsil-derived mesenchymal stem cells into chondrocytes through ERK signaling

  • Kim, Hye Ryeong (Department of Biological Science, Dong-A University) ;
  • Choi, Hyeongrok (Department of Biological Science, Dong-A University) ;
  • Park, Soon Yong (Department of Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Song, Young-Chul (Department of Physiology, Pusan National University School of Medicine) ;
  • Kim, Jae-Ho (Department of Physiology, Pusan National University School of Medicine) ;
  • Shim, Sangin (Department of Agronomy, Gyeongsang National University) ;
  • Jun, Woojin (Department of Food and Nutrition, Chonnam National University) ;
  • Kim, Kyung-Jin (Department of Life Sciences, College of Natural Sciences, Kyungpook National University) ;
  • Han, Jin (Department of Physiology, College of Medicine, Inje University) ;
  • Chi, Seung-Wook (Disease Target Structure Research Center, KRIBB) ;
  • Leem, Sun-Hee (Department of Health Sciences, Dong-A University) ;
  • Chung, Jin Woong (Department of Biological Science, Dong-A University)
  • Received : 2021.12.03
  • Accepted : 2022.01.06
  • Published : 2022.05.31
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Abstract

It is well-known that some species of lizard have an exceptional ability known as caudal autotomy (voluntary self-amputation of the tail) as an anti-predation mechanism. After amputation occurs, they can regenerate their new tails in a few days. The new tail section is generally shorter than the original one and is composed of cartilage rather than vertebrae bone. In addition, the skin of the regenerated tail distinctly differs from its original appearance. We performed a proteomics analysis for extracts derived from regenerating lizard tail tissues after amputation and found that endoplasmin (ENPL) was the main factor among proteins up-regulated in expression during regeneration. Thus, we performed further experiments to determine whether ENPL could induce chondrogenesis of tonsil-derived mesenchymal stem cells (T-MSCs). In this study, we found that chondrogenic differentiation was associated with an increase of ENPL expression by ER stress. We also found that ENPL was involved in chondrogenic differentiation of T-MSCs by suppressing extracellular signal-regulated kinase (ERK) phosphorylation.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF-2020R1F1A1070475) and the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM9952213).

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