The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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In-vitro Neuroprotective Effect of Aricumin(Turmeric extract)

아리큐민의 In-vitro 신경보호 효과

  • 윤남규 (주식회사 아리비앤씨 기업부설연구소) ;
  • 김병권 (주식회사 아리비앤씨) ;
  • 유현열 (한국건설생활환경시험연구원) ;
  • 서보승 (주식회사 아리바이오) ;
  • 신창호 (성균관대학교 스포츠과학과) ;
  • 김관규 (용인대학교 체육학과) ;
  • 이한주 (주식회사 아리비앤씨)
  • Received : 2022.04.25
  • Accepted : 2022.05.08
  • Published : 2022.05.31
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Abstract

This study was conducted on curcumin which had increased bioavailability as a potential AChE inhibitor for the treatment of neurodegenerative diseases. The purpose of this study is to confirm the in vitro neuroprotective effect on Aricumin (turmeric extract). To confirm the neuroprotective effect, AChE inhibition for Aricumin was evaluated, and cell viability was analyzed for HT-22cell, and oxidative stress (glutamate, H2O2)-induced HT-22 cytotoxicity was evaluated. As a result of the change in the AChE inhibition rate of Aricumin (Turmeric extract), it was confirmed that Aricumin at a concentration of 39.06㎍/ml or higher inhibited AChE activity by about 20% and more. And it was confirmed that the cytotoxicity of HT-22 cells induced by oxidative stress (Gluamate 5 mM and H2O2 500 µM) was significantly inhibited from 0.01 to 0.1 mg/ml concentration (p<005). These results suggest that Aricumin (turmeric extract) have potential neuroprotective effects.

퇴행성 신경질환 치료를 위한 AChE inhibitor 관련 연구로써 생물학적 유용성을 높인 커큐민에 대한 연구를 수행하게 되었다. 본 연구의 목적은 아리큐민(강황추출물)에 대한 in vitro 신경보호 효과를 확인하는데 있다. 신경보호효과를 확인하기 위해 아리큐민(강황추출물)에 대한 AChE inhibition을 평가하였고, HT-22 세포에 대한 세포생존율을 분석하였으며, 산화스트레스(glutamate, H2O2) 유발에 따른 HT-22 세포생존을 확인하였다. 아리큐민(강황추출물)의 AChE 저해율 변화결과 아리큐민 39.06㎍/㎖ 이상의 농도에서 약 20% 이상의 AChE 활성을 저해하는 것으로 확인하였다. 그리고 산화스트레스(glutamate 5 mM 및 H2O2 500 µM) 유발 HT-22 cell의 세포 독성을 0.01~0.1 mg/ml 농도에서부터 유의하게 억제하는 것을 확인하였다(p<0.05). 이와 같은 결과로 볼 때 아리큐민(강황추출물)은 신경보호 효과 효능이 우수한 것으로 확인되었다.

Keywords

Acknowledgement

이 논문은 중소벤처기업부 산연협력(사업화 R&D) 지원(S2889318)에 의하여 연구되었음.

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