Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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E3 ligase BRUTUS Is a Negative Regulator for the Cellular Energy Level and the Expression of Energy Metabolism-Related Genes Encoded by Two Organellar Genomes in Leaf Tissues

  • Choi, Bongsoo (Department of Life Science, Pohang University of Science and Technology) ;
  • Hyeon, Do Young (School of Biological Sciences, Seoul National University) ;
  • Lee, Juhun (Department of Life Science, Pohang University of Science and Technology) ;
  • Long, Terri A. (Department of Plant and Microbial Biology, North Carolina State University) ;
  • Hwang, Daehee (School of Biological Sciences, Seoul National University) ;
  • Hwang, Inhwan (Department of Life Science, Pohang University of Science and Technology)
  • Received : 2021.11.21
  • Accepted : 2021.12.26
  • Published : 2022.05.31
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Abstract

E3 ligase BRUTUS (BTS), a putative iron sensor, is expressed in both root and shoot tissues in seedlings of Arabidopsis thaliana. The role of BTS in root tissues has been well established. However, its role in shoot tissues has been scarcely studied. Comparative transcriptome analysis with shoot and root tissues revealed that BTS is involved in regulating energy metabolism by modulating expression of mitochondrial and chloroplast genes in shoot tissues. Moreover, in shoot tissues of bts-1 plants, levels of ADP and ATP and the ratio of ADP/ATP were greatly increased with a concomitant decrease in levels of soluble sugar and starch. The decreased starch level in bts-1 shoot tissues was restored to the level of shoot tissues of wild-type plants upon vanadate treatment. Through this study, we expand the role of BTS to regulation of energy metabolism in the shoot in addition to its role of iron deficiency response in roots.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) and funded by a grant from the Korea government (MSIT) (No. 2019R1A2B5B03099982).

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