Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Fast and Precise: How to Measure Meiotic Crossovers in Arabidopsis

  • Kim, Heejin (Department of Life Sciences, Pohang University of Science and Technology) ;
  • Choi, Kyuha (Department of Life Sciences, Pohang University of Science and Technology)
  • Received : 2021.12.29
  • Accepted : 2022.03.04
  • Published : 2022.05.31
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Abstract

During meiosis, homologous chromosomes (homologs) pair and undergo genetic recombination via assembly and disassembly of the synaptonemal complex. Meiotic recombination is initiated by excess formation of DNA double-strand breaks (DSBs), among which a subset are repaired by reciprocal genetic exchange, called crossovers (COs). COs generate genetic variations across generations, profoundly affecting genetic diversity and breeding. At least one CO between homologs is essential for the first meiotic chromosome segregation, but generally only one and fewer than three inter-homolog COs occur in plants. CO frequency and distribution are biased along chromosomes, suppressed in centromeres, and controlled by pro-CO, anti-CO, and epigenetic factors. Accurate and high-throughput detection of COs is important for our understanding of CO formation and chromosome behavior. Here, we review advanced approaches that enable precise measurement of the location, frequency, and genomic landscapes of COs in plants, with a focus on Arabidopsis thaliana.

Keywords

Acknowledgement

We thank Choi lab members for their critical reading and helpful comments. This work was funded by the Suh Kyungbae Foundation (SUHF) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education NRF-2020R1A2C2007763.

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