Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Medium optimization for growth of Bacillus amyloliquefaciens ISP-5 strain and evaluation of plant growth promotion using lettuce

Bacillus amyloliquefaciens ISP-5 균주의 배지 최적화 및 상추를 이용한 식물 생장 촉진 평가

  • Kang-Hyun Choi (Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM)) ;
  • Sun Il Seo (Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM)) ;
  • Haeseong Park (Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM)) ;
  • Ji-hwan Lim (Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM)) ;
  • Pyoung Il Kim (Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM))
  • 최강현 ((재)농축산용미생물산업지원육성센터) ;
  • 서선일 ((재)농축산용미생물산업지원육성센터) ;
  • 박해성 ((재)농축산용미생물산업지원육성센터) ;
  • 임지환 ((재)농축산용미생물산업지원육성센터) ;
  • 김평일 ((재)농축산용미생물산업지원육성센터)
  • Received : 2022.12.12
  • Accepted : 2022.12.28
  • Published : 2022.12.31
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Abstract

Bacillus sp. is a useful strain for agriculture because it promotes plant growth and controls plant pathogens through a variety of mechanisms. In this study, we obtained a microbial preparation with a high number of viable cells by culturing newly isolated soil bacteria on an optimized medium. Subsequently, we applied this preparation to lettuce to enhance its growth and yield. First, B. amyloliquefaciens ISP-5 was isolated from soil. Next, optimization of culture medium was carried out using 5 L scale fermenters. When culturing B. amyloliquefaciens ISP-5 on this optimized medium, the number of viable cells was approximately 1000 times higher than that obtained from culturing on the commercial medium. Afterwards, the plant growth promotion properties of the ISP-5 strain were evaluated using lettuce as a test plant. Foliar spray treatment of lettuce was carried out by inoculating half the standard concentration suspension (0.5 × 107 cfu/ml). As a result, leaf width increased by 8.6% and leaf length increased by 12.9% compared to the control group. Live weight also increased by 24.2% and dry weight by 23.9%. Considering the results from field test, B. amyloliquefaciens ISP-5 showed potential as a plant growth-promoting bacteria.

본 연구에서는 Bacillus amyloliquefaciens ISP-5 균주의 최적배지조성을 확인하기 위해 5종의 탄소원(glucose, fructose, sucrose, maltose, corn starch)과 5종의 질소원(yeast extract, peptone, tryptone, soytone, soy bean flour)을 이용하여 각각의 생육을 측정하였다. 그 결과 glucose와 soy bean flour를 각각 최적 탄소원과 질소원으로 확립하였으며 최적 배지를 이용하였을 때 상업용 배지(TSB)에 비해 약 2500배의 생육을 확인할 수 있었다. B. amyloliquefaciens ISP-5 배양액을 상추에 엽면 살포하였을 때 상추의 엽폭과 엽장은 대조군에 비해 8.6%, 12.9%의 증가하였으며, 생체중과 건물중은 대조군에 비해 각각 24.2%, 23.9% 증가하였다. 이러한 결과들을 종합해보았을 때 B. amyloloiquefaciens ISP-5 균주를 이용한 미생물 제제는 상추용 식물생장촉진제로써 활용할 수 있을 것이라 기대된다.

Keywords

Acknowledgement

본 연구는 농림식품기획평가원(과제 번호: 320102032HD020)의 지원에 의하여 이루어졌습니다.

References

  1. Birbir M, Calli B, Mertoglu B, Bardavid RE, Oren A, Ogmen MN and Ogan A (2007) Extremely halophilic Archaea from Tuz Lake, Turkey, and the adjacent Kaldirim and Kayacik salterns. World J Microbiol Biotech 23:309-316 https://doi.org/10.1007/s11274-006-9223-4
  2. Bong KM, Kim JM, Yoo JH, Park IC, Lee CW, Kim PI (2016) Mass cultivation and secondary metabolite analysis of Rhodobacter capsultatus PS-2. KSBB J 31:158-164 https://doi.org/10.7841/ksbbj.2016.31.3.158
  3. Costacurta A, Mazzafera P, Rosato Y (1998) Indole-3-acetic acid biosynthesis by Xanthomonas axonopodis pv. citri is increased in the presence of plant leaf extracts. FEMS Microbiol Lett 159:215-220 https://doi.org/10.1111/j.1574-6968.1998.tb12863.x
  4. Guo B, Wang Y, Sun X, Tang K (2008) Bioactive natural products from endophytes: A review. Applied Biochemistry and Microbiology 44:153-158
  5. Idris EE, Iglesias DJ, Talon M, Borriss R (2007) Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 20(6):619-626
  6. Khan SA, Hamayun M, Yoon HJ (2008) Plant growth promotion and Penicillium citrinum. BMC Microbiol 8:231
  7. Kim GH, Oh SO, Hur JS, Yum KJ, Koh YJ (2006) Optimum cultivation conditions for mass production of an antagonistic bacterium Bacillus subtilis BD0310 for development of a microbial agent controlling gray blight of tea plants. Korean Society of Plant Pathology 12:85-90
  8. Kim JH, Choi YH, Kang SJ, Joo GJ, Suh JS, Lim TH (2003) Isolation of Bacillus amyloliquefaciens MJ-3 and Its Effect on the Early Growth Promotion of Red Pepper Plug Seedlings in Compost 13(5):582-589
  9. Kim WS, Park JS (2013) Selection and control effect of environmental friendly organic materials for controlling the ginseng alternaria blight. Korean Journal of Medicinal Crop Science 21:388-393 https://doi.org/10.7783/KJMCS.2013.21.5.388
  10. Lee HJ (2009) Monitoring of ergosterol biosynthesis inhibitor (EBI) pesticide residues in commercial agricultural products and risk assessment. Korean J Food Sci 38(12):1779-1784 https://doi.org/10.3746/jkfn.2009.38.12.1779
  11. Lehotay SJ, Son KA, Kwon H, Koesukwiwat U, Fu W, Mastovska K, Hoh E, Leepipatpiboon N (2010) Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. J Chromatogr A 1217:2548-2560 https://doi.org/10.1016/j.chroma.2010.01.044
  12. Reyes I, Bernier L, Antoun H (2002) Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum. Microbial Ecology 44:39-48 https://doi.org/10.1007/s00248-002-1001-8
  13. Shin TS, Park CK, Lee SH, Han KH (2005) Effect of age on chemical composition in sun-dried salts. Kor J Food Sci Technol 37:312-317
  14. Sorokin DY, Tourova TP, Lysenko AM, uyzer G (2006) Diversity of culturable halophilic sulfur oxidizing bacteria in hypersaline habitats. Microbiology 152:3013-3023 https://doi.org/10.1099/mic.0.29106-0
  15. Winder RS, Wheeler JJ, Conder N, Otvos IS, Nevill R, Duan L (2003) Microencapsulation: A strategy for formulation of inoculum. Biocontrol Sci Technol 13:155-169 https://doi.org/10.1080/0958315021000073439
  16. Yanez-Mendizabal V, Vinas I, Usall J, Torres R, Solsona C (2012) Formulation development of the biocontrol agent Bacillus subtilis strain CPA-8 by spray-drying. J Appl Microbiol 112:954-965 https://doi.org/10.1111/j.1365-2672.2012.05258.x