KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Determination of Miliacin from Proso Millet Oil by GC/MS

GC/MS를 이용한 기장 기름의 밀리아신 함량 분석

  • 안연주 (국립식량과학원 남부작물부 밭작물개발과) ;
  • 이병원 (국립식량과학원 남부작물부 밭작물개발과) ;
  • 추지호 (국립식량과학원 남부작물부 밭작물개발과) ;
  • 송석보 (국립식량과학원 남부작물부 밭작물개발과) ;
  • 김지영 (국립식량과학원 남부작물부 밭작물개발과) ;
  • 주영광 (국립식량과학원 남부작물부 밭작물개발과) ;
  • 한상익 (국립식량과학원 남부작물부 밭작물개발과)
  • Received : 2022.11.30
  • Accepted : 2022.12.05
  • Published : 2022.12.01
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Abstract

Proso millet (Pacnicum miliaceum L.) has a various of functional substances, so the demand is increasing as interest in human health benefits. In particular, miliacin, a triterpenoid in proso millet, is known to be effective in hair loss due to its promoting metabolism and proliferation of keratinocytes and showing protective effects from apoptosis. In this study, proso millet oil was extracted and analyzed by GC/MS using the extraction method of unsaponifiable substances after saponification reaction. The components of proso millet oil were confirmed through qualitative analysis by GC/MS. The miliacin content of 5 varieties of proso millet and 2 varieties of foxtail for comparison was analyzed. In the result, components of pentacyclic triterpenes such as β-amyrine and lupeol were detected in proso millet, and the content of miliacin was highest in Hallachal at 370.38±0.04 ㎍/100 mg oil. In addition, the content of miliacin was not detected in Samdachal and Samdamae, which are varieties of foxtail millet.

기장에 있는 트리테르페노이드류인 밀리아신 성분은 각질 세포의 증식을 촉진하는 등의 특성을 가지고 있어 탈모에 효과가 있다고 알려져 있다. 이 연구에서는 기장의 기름을 추출하여 검화 반응을 거쳐 불검화물의 추출 방법을 이용하여 GC/MS로 분석하였다. 기장 기름 성분을 GC/MS의 정성분석을 통해 확인하였으며, 기장 5품종과 비교를 위한 조 2품종의 밀리아신 함량을 분석하였다. 1. 기장에서는 β-amyrine, lupeol 등의 pentacyclic triterpene 류의 성분들이 검출되었다. 2. 밀리아신 함량은 기장의 한라찰에서 370.38±0.04 ㎍/100 mg oil으로 가장 높은 함량을 나타내었다. 3. 특히 조의 품종인 삼다찰과 삼다메에서는 밀리아신 함량이 검출되지 않았다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 국립식량과학원 연구 사업(과제명: 기능성 고함유 잡곡 실용화 모델 개발, 과제번호: PJ014565012022)의 지원에 의해 이루어진 결과의 일부이며, 이에 감사드립니다.

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