한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제32권2호
  • /
  • Pages.1-17
  • /
  • 2022
  • /
  • 1229-1854(pISSN)
  • /
  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
권호 표지
DOI QR코드

DOI QR Code

황금, 어성초를 배합한 익수영진고가미 한약재배합물의 베타글루칸, 진세노사이드 함량, 2,2-diphenyl-1-picrylhydrazyl Free Radical 소거 활성, 항염 활성 및 안전성 연구

A Study on 𝛽-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl Free Radical Scavenging Activity, Anti-inflammatory Activity and Safety of Herbal Medicine Mix - Iksooyoungjingogami with Scutellariae Radix and Houttuynia cordata Thunb

  • 김명훈 (동신대학교 한의과대학 경혈진단학교실) ;
  • 문양선 ((유)나우리) ;
  • 강상미 (동신대학교 한의과대학 경혈진단학교실) ;
  • 김형석 (페토바이오) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈진단학교실)
  • Kim, Myeong-Hun (Department of Meridian & Acupoint.Diagnosis of Korean Medicine, College of Korean Medicine, Dongshin University) ;
  • Moon, Yang-Seon (Nawoori (Ltd.)) ;
  • Kang, Sang-Mi (Department of Meridian & Acupoint.Diagnosis of Korean Medicine, College of Korean Medicine, Dongshin University) ;
  • Kim, Heyong-Seok (Petobio (Ltd.)) ;
  • Kim, Seon-Jong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Na, Chang-Su (Department of Meridian & Acupoint.Diagnosis of Korean Medicine, College of Korean Medicine, Dongshin University)
  • 투고 : 2022.02.17
  • 심사 : 2022.02.28
  • 발행 : 2022.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives This study was conducted to investigate the beta-glucan & ginsenoside content, antioxidant activity, anti-inflammatory effect and safety of herbal medicine mix. Methods The marker compounds contents, antioxidant activity and safety of herbal medicine mix were tested. The contents of beta-glucan and ginsenoside Rg3 were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, anti-inflammatory and a safety test was conducted via single dose toxicity assessment. Results Analyzing the contents of marker compounds showed 362.3 mg/g of beta-glucan, and 0.4184 mg/g of ginsenoside Rg3. In the DPPH free radical scavenging activity, the IC50 of herbal medicine mix, was 0.146%. The scavenging activity of herbal medicine mix was 88.28% activity at 0.5% concentration, and 90.61% activity at 5% concentration. In the lipopolysaccharides (LPS) anti-inflammatory test, the herbal remix showed a significant decrease in tumor necrosis factor-alpha (TNF-𝛼) and interleukin-6 (IL-6) compared to the LPS-induced group. In the single dose toxicity test of herbal medicine mix, a dose of 2,000 mg/kg body weight (BW) was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg BW or higher for both females and males. Conclusions The results of the experiment on herbal medicine mix showed that the marker compounds contents were beta-glucan and ginsenoside Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, anti-inflammatory effect was observed through TNF-𝛼 and IL-6 measurement, and safety was confirmed through the single dose toxicity assessment.

키워드

과제정보

This study was supported by a grant of the SME R&D project for the start-up & grow stage company, Ministry of SMEs and Startups (S2975868).

참고문헌

  1. Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine. 2010;10(1):1-8. https://doi.org/10.1186/1472-6882-10-1
  2. Kim JH, Jeong CH, Choi GN, Kwak JH, Choi SG, Heo HJ. Antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis using an in vitro system. Korean Journal of Food Science and Technology. 2009;41(6):712-6.
  3. Sim MO, Lee HJ, Jang JH, Lee HE, Jung HK, Kim TM, No JH, Jung J, Jung DE, Cho HW. Anti-inflammatory and antioxidant effects of Spiraea prunifolia Sieb. et Zucc. var. simpliciflora Nakai in RAW 264.7 cells. Korean Journal of Plant Resources. 2017;30(4):335-42. https://doi.org/10.7732/KJPR.2017.30.4.335
  4. Doh ES, Chang JP, Kil KJ, Choi MS, Yang JK, Yun CW, Jeong SM, Jung YH, Lee GH. Antioxidative activity and cytotoxicity of fermented Allium victorialis L. extract. Korean Journal of Plant Resources. 2011;24(1):30-9. https://doi.org/10.7732/KJPR.2011.24.1.030
  5. Je JY. Chitosan-phytochemical conjugates: preparation, antioxidant, and NO inhibition in LPS-stimulated macrophages. J. Chitin Chitosan. 2015;20(4):245-50. https://doi.org/10.17642/jcc.20.4.4
  6. Soory M. Relevance of nutritional antioxidants in metabolic syndrome, ageing and cancer: potential for therapeutic targeting. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders). 2009;9(4):400-14. https://doi.org/10.2174/187152609788922537
  7. Kim JH. Identification and characterization of fibrinolytic compound from Cornus officinalis S. et Z. Korean Journal of Plant Resources. 2020;33(4):237-44. https://doi.org/10.7732/KJPR.2020.33.4.237
  8. Sakagami H, Kobayashi M, Chien CH, Kanegae H, Kawase M. Selective toxicity and type of cell death induced by various natural and synthetic compounds in oral squamous cell carcinoma. In Vivo. 2007;21(2):311-20.
  9. Heo J. Dongeuibogam. Seoul:Namsandang. 1989:78.
  10. Kim MD. The literature study on the efficacy and manufacturing process of Gyeongoggo. Journal of Korean Medical Classics. 2011;24(2):51-64. https://doi.org/10.14369/SKMC.2011.24.2.051
  11. Choi JH, Kim HM, Song YS, Park SG, Kim JJ, Lee CK. Anti-aging effects Saccharomyces fermented modified Kyungohkgo extract on skin. The Korea Journal of Herbology. 2007;22(4):219-25.
  12. Park JH. Protective effects of Iksuyongjingo against neurotoxicity induced by 6-hydroxydopamine in PC12 cells [dissertation]. Seoul:Kyung-Hee University; 2009.
  13. Lee YM, Kim WI, Choi DH, Kim MR, Moon YS, Kim JE, Youn DH, Son HS, Na CS. The effect of prescriptions prepared by adding medicinal herbs(Rehmannia glutinosa Residue, Poria cocos Bark) and fermenting herbal materials based on formulas Iksuyoungjingo on immunological activity. Herbal Formula Science. 2017;26(1): 13-26. https://doi.org/10.14374/HFS.2018.26.1.13
  14. Na CS, Shin W, Lee YM, Moon YS, Noh HK, Seo SH, Son HS. Effect of original kyungokgo & iksuyongjingo plus Sparassis crispa on antioxidant, immunity improvement and sensory evaluation. The Korea Journal of Herbology. 2016;31(4):43-51. https://doi.org/10.6116/KJH.2016.31.4.43.
  15. Lee YM, Moon YS, Park HM, Kim HS, Ro WB, Na CS. A study on the β-glucan, ginsenoside content, DPPH free radical scavenging activity and single dose toxicity assessment of modified Kyungohkgo. J Korean Med Rehabil. 2021;31(1):95-108.
  16. Lim SC. Effects of Baikal Skullcap (Scutellaria baicalensis Georgi) extracts on the recovery of liver function in CCl4-exposed rats. Korean Journal of Plant Resources. 2010;23(1):54-9.
  17. Yoon HJ, Park YS. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. Journal of the Korean Society of Food Science and Nutrition. 2010;39(2):219-26. https://doi.org/10.3746/JKFN.2010.39.2.219
  18. Kim YH, Park YS. Effect of Scutellaria baicalensis water extract on antioxidative activity and epidermal thickness in DNCB-induced allergic contact dermatitis animal model. Journal of the Korean Society of Food Science and Nutrition. 2006;35(5):543-8. https://doi.org/10.3746/JKFN.2006.35.5.543
  19. Proebstle A, Lotter H, Wagner-Redecker W, Matthiesen U, Bauer R. Identification of lipophilic constituents with antiinflammatory activity from Houttuynia cordata. Planta Medica. 1993;59(S1):A663-4.
  20. Ministry of Food and Drug Safety. Ministry of Food and Drug Safety White Paper. 2021 [cited 2021 Aug]. Available from: URL: https://www.mfds.go.kr/brd/m_218/view.do?seq=33414&srchFr=&srchTo=&srchWord=&srchTp=&.htm.
  21. Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002;82(1):47-95. https://doi.org/10.1152/physrev.00018.2001
  22. Halliewell B, Gutterridge JM. Roles of free radicals and catalytic metal ions in human disease. In: Fleischer S, Packer L, eds. An overreview, methods enzymol. New York:Academic Press. 1990:186:1-85.
  23. Verckei A, Toncsev H, Feher J, Hajdu E. Relationship between the extent of coronary artery disease and indicators of free radical activity. Clin Cardiol. 1992;15(9): 706-7. https://doi.org/10.1002/clc.4960150920
  24. Aitken RJ, Bukingham D, Harkiss D. Use of xanthine oxidase free radical generating system to investigate the cytotoxic effect of reactive oxygen species on human spermatozoa. J Reprod Fertil. 1993;97(2):441-50. https://doi.org/10.1530/jrf.0.0970441
  25. Baublis AJ, Lu C, Clydesdale FM, Decker EA. Potential of wheat-based breakfast grains as a source of dietary antioxidants. J Am Coll Nutr. 2000;19(sup3):308S-11S. https://doi.org/10.1080/07315724.2000.10718965
  26. Cho W, Nam JW, Kang HJ, Windono T, Seo EK, Lee KT. Zedoarondiol isolated from the rhizoma of Curcuma heyneana is involved in the inhibition of iNOS, COX-2 and pro-inflammatory cytokines via the downregulation of NF-κB pathway in LPS-stimulated murine macrophages. Int Immunopharmacol. 2009;9(9):1049-57. https://doi.org/10.1016/j.intimp.2009.04.012
  27. Lee JW, Kang YJ. Anti-inflammatory effects of Abeliophyllum distichum flower extract and associated MAPKs and NF-κB pathway in RAW 264.7 cells. Korean J Plant Res. 2018;31(3):202-10. https://doi.org/10.7732/KJPR.2018.31.3.202
  28. Kim CH, Lee YK, Jeong JW, Hwang BS, Jeong YT, Oh YT, Cho PY, Kang CH. Anti-inflammatory effects of Hemistepta lyrata Bunge in LPS-stimulated RAW 264.7 cells through regulation of MAPK signaling pathway. Korean J Plant Res. 2021;34(1):23-30. https://doi.org/10.7732/KJPR.2021.34.1.023
  29. Park JC, Han HS, Lee SB, Lee KT. Inhibitory effects of kaempferol-7-O-β-D-glucoside on LPS-induced NO, PGE2 and inflammatory cytokines production in RAW 264.7 macrophages. Kor J Pharmacogn. 2016;47(4):295-300.
  30. Radi RB, Beckman JS, Bu sh KM, Freeman BA. Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J Biology Chemistry. 1991;266(7):4244-50. https://doi.org/10.1016/S0021-9258(20)64313-7
  31. Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macro phages. Life Sci. 2001;69(6):625-35. https://doi.org/10.1016/S0024-3205(01)01154-7
  32. Min JY, Park YK. Effect of Dipsaci Radix water extract on LPS-induced inflammatory response in RAW 264.7 mouse macrophages. Kor J Herb. 2009;24(4):189-95.
  33. Arendt-Nielsen L, Fernandez-de-las-Penas C, Graven-Nielsen T. Basic aspects of musculoskeletal pain: from acute to chronic pain. J Man Manip Ther. 2011;19(4):186-93. https://doi.org/10.1179/106698111X13129729551903
  34. Gallo J, Raska M, Kriegova E, Goodman SB. Inflammation and its resolution and the musculoskeletal system. J Orthop Translat. 2017;10:52-67. https://doi.org/10.1016/j.jot.2017.05.007
  35. Jeong DH, Kim WR, Kim MJ, Kang BK, Ahn DH. Anti-inflammatory activity of methanol extract and nhexane fraction mojabanchromanol b from Myagropsis myagroides. Life Sci. 2014;114(1):12-9. https://doi.org/10.1016/j.lfs.2014.07.036
  36. Masaki M, Matsushita M, Wakitani K. Inhibitory effect of JTE-522, a novel prostaglandin H synthase-2 inhibitor, on adjuvant-induced arthritis and bone changes in rats. Inflamm Res. 1998;47(4):187-92. https://doi.org/10.1007/s000110050316
  37. Lim CW, Kang KK, Yoo YB, Kim BH, Bae SH. Dietary fiber and β-glucan contents of Sparassis crispa fruit fermented with Lactobacillus brevis and Monascus pilosus. J Korean Soc Food Sci Nutr. 2012;41(12):1740-6. https://doi.org/10.3746/JKFN.2012.41.12.1740
  38. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka G, Nishooka I. Study on the inhibitory effects of tannins and flavonoids against the 1,1-diphenyl-2picrylhydrazyl radical. Biochemical Pharmacology. 1998;56:213-22. https://doi.org/10.1016/S0006-2952(98)00128-2
  39. Woo HS, Lee SM, Heo JD, Lee MS, Kim YS, Kim DW. Anti-inflammatory activity of extracts of Hovenia dulcis on lipopolysaccharides-stimulated RAW264.7 cells. Korean J Plant Res. 2018;31(5):466-77. https://doi.org/10.7732/KJPR.2018.31.5.466
  40. Abbvie Global. Dual variable domain immunoglobulin [Internet] 2019 [cited 2019 Feb 25]. Available from: URL: https://www.abbvie.co.kr/our-science/therapeutic-focus-areas/immunology/immunology-discovery-development.html.