Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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A Study on 𝛽-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl Free Radical Scavenging Activity, Anti-inflammatory Activity and Safety of Herbal Medicine Mix - Iksooyoungjingogami with Scutellariae Radix and Houttuynia cordata Thunb

황금, 어성초를 배합한 익수영진고가미 한약재배합물의 베타글루칸, 진세노사이드 함량, 2,2-diphenyl-1-picrylhydrazyl Free Radical 소거 활성, 항염 활성 및 안전성 연구

  • Kim, Myeong-Hun (Department of Meridian & Acupoint.Diagnosis of Korean Medicine, College of Korean Medicine, Dongshin University) ;
  • Moon, Yang-Seon (Nawoori (Ltd.)) ;
  • Kang, Sang-Mi (Department of Meridian & Acupoint.Diagnosis of Korean Medicine, College of Korean Medicine, Dongshin University) ;
  • Kim, Heyong-Seok (Petobio (Ltd.)) ;
  • Kim, Seon-Jong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Na, Chang-Su (Department of Meridian & Acupoint.Diagnosis of Korean Medicine, College of Korean Medicine, Dongshin University)
  • 김명훈 (동신대학교 한의과대학 경혈진단학교실) ;
  • 문양선 ((유)나우리) ;
  • 강상미 (동신대학교 한의과대학 경혈진단학교실) ;
  • 김형석 (페토바이오) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈진단학교실)
  • Received : 2022.02.17
  • Accepted : 2022.02.28
  • Published : 2022.04.30
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Abstract

Objectives This study was conducted to investigate the beta-glucan & ginsenoside content, antioxidant activity, anti-inflammatory effect and safety of herbal medicine mix. Methods The marker compounds contents, antioxidant activity and safety of herbal medicine mix were tested. The contents of beta-glucan and ginsenoside Rg3 were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, anti-inflammatory and a safety test was conducted via single dose toxicity assessment. Results Analyzing the contents of marker compounds showed 362.3 mg/g of beta-glucan, and 0.4184 mg/g of ginsenoside Rg3. In the DPPH free radical scavenging activity, the IC50 of herbal medicine mix, was 0.146%. The scavenging activity of herbal medicine mix was 88.28% activity at 0.5% concentration, and 90.61% activity at 5% concentration. In the lipopolysaccharides (LPS) anti-inflammatory test, the herbal remix showed a significant decrease in tumor necrosis factor-alpha (TNF-𝛼) and interleukin-6 (IL-6) compared to the LPS-induced group. In the single dose toxicity test of herbal medicine mix, a dose of 2,000 mg/kg body weight (BW) was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg BW or higher for both females and males. Conclusions The results of the experiment on herbal medicine mix showed that the marker compounds contents were beta-glucan and ginsenoside Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, anti-inflammatory effect was observed through TNF-𝛼 and IL-6 measurement, and safety was confirmed through the single dose toxicity assessment.

Keywords

Acknowledgement

This study was supported by a grant of the SME R&D project for the start-up & grow stage company, Ministry of SMEs and Startups (S2975868).

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