DOI QR코드

DOI QR Code

DNA Barcoding of Rocinela niponia (Isopoda, Cymothooidea, Aegidae) from South Korea

  • Kim, Sung Hoon (Division of Ocean Sciences, Korea Polar Research Institute) ;
  • Choi, Hyun Ki (Animal Resources Division, National Institute of Biological Resources) ;
  • Kim, Jong Guk (Marine Ecosystem Research Center, Korea Institute of Ocean Science and Technology)
  • Received : 2022.01.12
  • Accepted : 2022.03.23
  • Published : 2022.04.30

Abstract

An aegid species, Rocinela niponia Richardson, 1909, is a Far Eastern species known from Korean and Japanese waters. In this study, mitochondrial cytochrome c oxidase subunit I (COI) sequences of R. niponia were determined based on four specimens collected from the subtidal zone of Chujado Island, South Korea. We compared DNA barcoding data of this species with its congeners. As a result, there was no intra-specific genetic distance between the four COI sequences of R. niponia. Inter-specific distances between R. niponia and other five aegid species ranged from 23.8% to 35.6%. Morphological diagnosis and images of R. niponia are also provided as a valuable contribution toward the identification of Rocinela species in further taxonomic and ecological studies.

Keywords

Acknowledgement

This study was supported by a grant (NIBR202203106) from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) of the Republic of Korea.

References

  1. Bruce NL, 2009. The marine fauna of New Zealand: Isopoda, Aegidae (Crustacea). NIWA Biodiversity Memoir, 122:1-252.
  2. Brusca RC, France SC, 1992. The genus Rocinela (Crustacea: Isopoda: Aegidae) in the tropical eastern Pacific. Zoological Journal of the Linnean Society, 106:231-275. https://doi.org/10.1111/j.1096-3642.1992.tb01248.x
  3. Cardoso L, Martins ML, Golzio JESA, Bomfim CNC, De Oliveira RLM, Santos LBG, Lacerda ACF, 2017. Rocinela signata (Isopoda: Aegidae) parasitizing the gills of the spotted goatfish Pseudupeneus maculatus(Actinopterygii: Mullidae) in Northeastern Brazil. Anais da Academia Brasileira de Ciencias, 89:2075-2080. https://doi.org/10.1590/0001-3765201720150677
  4. Costa FO, deWaard JR, Boutillier J, Ratnasingham S, Dooh RT, Hajibabaei M, Hebert PDN, 2007. Biological identifications through DNA barcodes: the case of the Crustacea. Canadian Journal of Fisheries and Aquatic Sciences, 64:272-295. https://doi.org/10.1139/f07-008
  5. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3:294-299.
  6. Garzon-Ferreira J, 1990. An isopod, Rocinela signata (Crustacea: Isopoda: Aegidae), that attacks humans. Bulletin of Marine Science, 46:813-815.
  7. Hata H, Sogabe A, Tada S, Nishimoto R, Nakano R, Kohya N, Takeshima H, Kawanishi R, 2017. Molecular phylogeny of obligate fish parasites of the family Cymothoidae (Isopoda, Crustacea): evolution of the attachment mode to host fish and the habitat shift from saline water to freshwater. Marine Biology, 164:105. https://doi.org/10.1007/s00227-017-3138-5
  8. Kim SH, Choi HK, Kim JG, 2021. DNA barcoding of Eurydice longiantennata (Isopoda, Cymothooidea, Cirolanidae) from South Korea. Animal Systematics, Evolution and Diversity, 37:354-357. https://doi.org/10.5635/ASED.2021.37.4.032
  9. Kim SH, Yoon SM, 2020. Descriptions of two cymothoid isopods (Crustacea, Isopoda, Cymothoida) from Korean waters. Animal Systematics, Evolution and Diversity, 36:372-381. https://doi.org/10.5635/ASED.2020.36.4.076
  10. Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35:1547. https://doi.org/10.1093/molbev/msy096
  11. Kussakin OG, 1974. Fauna and ecology of isopods (Crustacea) from the intertidal zone of the Kurile Islands. In: Flora and Fauna of the Intertidal Zone of the Kurile Islands(Ed., Zhirmunsky AV). Novosibirsk, Nauka, pp. 227-275.
  12. Radulovici AE, Sainte-Marie B, Dufresne F, 2009. DNA barcoding of marine crustaceans from the Estuary and Gulf of St Lawrence: a regional-scale approach. Molecular Ecology Resources, 9:181-187. https://doi.org/10.1111/j.1755-0998.2009.02643.x
  13. Raupach MJ, Barco A, Steinke D, Beermann J, Laakmann S, Mohrbeck I, Neumann H, Kihara TC, Pointner K, Radulovici A, Segelken-Voigt A, Wesse C, Knebelsberger T, 2015. The application of DNA barcodes for the identification of marine crustaceans from the North Sea and adjacent regions. PLoS One, 10:e0139421. https://doi.org/10.1371/journal.pone.0139421
  14. Richardson H, 1909. Isopods collected in the northwest Pacific by the US Bureau of Fisheries Steamer "Albatross" in 1906. Proceedings of the United States National Museum, 37:75-129. https://doi.org/10.5479/si.00963801.37-1701.75
  15. Schioedte JC, Meinert F, 1879. Symbolae ad monographium Cymothoarum Crustaceorum Isopodum familiae. I. Aegidae. Naturhistorisk Tidsskrift, 3:321-414. https://doi.org/10.5962/bhl.title.10300
  16. Smit NJ, Bruce NL, Hadfield KA, 2019. Parasitic Crustacea: state of knowledge and future trends. Springer, Cham, pp. 1-481. https://doi.org/10.1007/978-3-030-17385-2
  17. Song JH, 2020. A new record of Porcellio scaber (Isopoda: Oniscidea: Porcellionidae) from South Korea, with notes on its variation. Animal Systematics, Evolution and Diversity, 36:309-315. https://doi.org/10.5635/ASED.2020.36.4.052
  18. Taiti S, Arnedo MA, Lew SE, Roderick GK, 2003. Evolution of terrestriality in Hawaiian species of the genus Ligia (Isopoda, Oniscidea). Crustaceana Monographs, 2:85-102. https://doi.org/10.1163/9789047412854_010
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution, 30:2725-2729. https://doi.org/10.1093/molbev/mst197