Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Isolation, Characterization, and Control of Pseudomonas kribbensis and Pantoea vagans that cause Soft-rot Disease Isolated from Chinese Cabbages

  • Received : 2021.12.09
  • Accepted : 2022.04.13
  • Published : 2022.04.30
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Abstract

The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.

세균성 무름병은 배추와 같은 채소의 주요 질병중의 하나이며, 특히 김치의 주원료인 배추의 저장에 있어서 발생할 경우, 품질 저하로 인한 경제적 손실을 유발한다. 우리는 무름병이 발생한 배추로부터 원인이 되는 세균들을 분리하였고, 해당 세균이 신선한 배추 조직에 인위적으로 접종하였을 경우, 무름병이 발생하는 것을 확인하였다. 해당 원인균 2종을 동정한 결과, Pseudomonas kribbensis와 Pantoea vagans로 확인되었다. 이러한 원인 세균을 제어하기 위하여 차아염소산수 처리와 항균활성 유산균의 배양 상등액을 이용하여 제어하는 테스트를 진행하였다. 본 테스트에 사용한 항균활성 유산균은 리스테리아와 같은 병원성 미생물과 Pseudomonas 속 계열을 저해하는 Lactobacillus plantarum PL203 유산균을 선별하여 사용하였다. 제어 테스트는 정상 배추 조직에 무름병 원인균 2종을 각각 105 cfu/g 이 되도록 접종한 후, 차아염소산수를 농도별로 분무하거나 항균활성 유산균 배양상등액을 0.5 mL 분무한 후, 25℃에서 5일간 배양한 결과, 차아염소산수 120 ppm 이상 첨가한 구간과 항균활성 유산균 배양 상등액을 처리한 구간에서 대조구와 비교하여 배추 무름 원인세균을 완전하게 제어하는 것을 확인하였다.

Keywords

Acknowledgement

This research was supported by 2020 Collaborative R&BD Program of The Food Industry Promotional Agency of Korea.

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