Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Glycine alleviated diquat-induced hepatic injury via inhibiting ferroptosis in weaned piglets

  • Hua, Hongwei (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Xu, Xiao (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Tian, Wei (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Li, Pei (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Zhu, Huiling (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Wang, Wenjun (College of Life Science, South-Central University for Nationalities) ;
  • Liu, Yulan (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Xiao, Kan (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University)
  • Received : 2021.01.28
  • Accepted : 2021.11.16
  • Published : 2022.06.01
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Abstract

Objective: The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury. Methods: Thirty-two piglets were assigned by a 2×2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected. Results: Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidant capacity and antioxidative enzyme activity significantly. Adding glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver. Conclusion: These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis.

Keywords

Acknowledgement

This study was supported by the Projects of Innovative Research Groups of the Natural Science Foundation of Hubei Province (No. 2019CFA015), and the National Natural Science Foundation of China (No. 31802076, and No. 31772615), the Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science (No. 201802).

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