Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Maternal undernutrition alters the skeletal muscle development and methylation of myogenic factors in goat offspring

  • Zhou, Xiaoling (College of Animal Science, Tarim University) ;
  • Yan, Qiongxian (Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Liu, Liling (College of Animal Science, Tarim University) ;
  • Chen, Genyuan (College of Animal Science, Tarim University) ;
  • Tang, Shaoxun (Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • He, Zhixiong (Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Tan, Zhiliang (Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
  • Received : 2021.06.22
  • Accepted : 2021.11.08
  • Published : 2022.06.01
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Abstract

Objective: The effects of maternal undernutrition during midgestation on muscle fiber histology, myosin heavy chain (MyHC) expression, methylation modification of myogenic factors, and the mammalian target of rapamycin (mTOR) signaling pathway in the skeletal muscles of prenatal and postnatal goats were examined. Methods: Twenty-four pregnant goats were assigned to a control (100% of the nutrients requirement, n = 12) or a restricted group (60% of the nutrients requirement, n = 12) between 45 and 100 days of gestation. Descendants were harvested at day 100 of gestation and at day 90 after birth to collect the femoris muscle tissue. Results: Maternal undernutrition increased (p<0.05) the fiber area of the vastus muscle in the fetuses and enhanced (p<0.01) the proportions of MyHCI and MyHCIIA fibers in offspring, while the proportion of MyHCIIX fibers was decreased (p<0.01). DNA methylation at the +530 cytosine-guanine dinucleotide (CpG) site of the myogenic factor 5 (MYF5) promoter in restricted fetuses was increased (p<0.05), but the methylation of the MYF5 gene at the +274,280 CpG site and of the myogenic differentiation (MYOD) gene at the +252 CpG site in restricted kids was reduced (p<0.05). mTOR protein signals were down-regulated (p<0.05) in the restricted offspring. Conclusion: Maternal undernutrition altered the muscle fiber type in offspring, but its relationship with methylation in the promoter regions of myogenic genes needs to be elucidated.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation (the People's Republic of China; 31760678, 31730092, and 31402105); and Hunan Innovative Province Construction Project (the People's Republic of China; 2019 RS3021).

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