현장농수산연구지 (Journal of Practical Agriculture & Fisheries Research)

국립한국농수산대학교 교육개발센터 (Korea National University of Agriculture and Fisheries)
권호 표지
DOI QR코드

DOI QR Code

해안가에 자생하는 순비기나무 열매의 증류추출을 통한 하이드로졸 성분분석

Analysis of Hydrosol Components through Distillation Extraction of the Sunbigi Tree(Vitex rotundifolia L.f.) Fruit of the Wild Birch Tree Native to the Coast

  • 정연옥 (수이케이 바이오랩) ;
  • 박노복 (국립한국농수산대학 원예학부)
  • Jung, Y.O. (Sooy-K Bio Lab, Research and Development) ;
  • Park, N.B. (Department of Floriculture, Korea National College of Agriculture and Fisheries)
  • 투고 : 2022.03.15
  • 심사 : 2022.04.07
  • 발행 : 2022.04.18
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

우리나라 남부와 서해 해안가를 중심으로 자생하고 있는 순비기나무는 자원 활용 측면에서 많은 활용 가치를 가지고 있다. 예로부터 민간에서 민간약재로 이용하거나 한약재로 사용되었다. 잎과 줄기에서도 강한 향이 나지만 열매에서도 강한 향이 나서 순비기나무 열매를 이용해 에센스오일을 추출하여 성분을 분석한 연구는 일부 있지만 하이드로졸을 추출하여 이에 대한 향기성분의 연구는 거의 없다. 이유는 열매가 딱딱하여 일반적인 추출방법으로는 함유된 유효 성분을 확인하는 것이 어렵기 때문이다. 따라서 본 연구는 추출방법을 달리하여 딱딱한 열매를 고온으로 추출하여 얻은 하이드로졸의 향에 함유된 성분을 분석한 결과는 다음과 같다. 1. 플라보노이드 함량이 가장 많은 추출조건은 ethanol로 30.57mg/g 이었고 다음으로 열수추출물로 18.26mg/g, 물 추출물이 가장 낮은 9.69mg/g이었다. 2. 순비기나무 열매를 증류추출한 결과 하이드로졸의 향기는 3-Methyl-2-butenoic acid, cyclobutyl ester, Eucalyptol, L-alpha-Terpineol, 1H-Cycloprop[e] azulen-7-ol, decahydro-1,1,7-trimethyl-4-methylene-, [1ar-(1a.alpha.,4a.alpha.,7.beta.,7a.beta.,7b. alpha.)]가 많은 것으로 나타났다.

Sunbigi tree(Vitex rotundifolia L.f.), which grows wild in the southern part of Korea and along the coast of the West Sea, has a lot of useful value in terms of resource utilization. Since ancient times, it has been used as folk medicine or herbal medicine in the private sector. Although the leaves and stems have a strong scent, the fruit also has a strong scent, so there are some studies on extracting essential oil from the fruit of Sunbigi tree and analyzing the ingredients, but there are few studies on the fragrance component by extracting hydrosol. The reason is that the fruits are hard and it is difficult to identify the active ingredients contained in the general extraction method. Therefore, in this study, the results of analyzing the components contained in the fragrance of hydrosol obtained by extracting hard fruits at high temperature by different extraction methods are as follows. 1. The extraction condition with the highest flavonoid content was 30.57 mg/g with ethanol, followed by hot water extract at 18.26 mg/g and water extract at 9.69 mg/g with the lowest. 2. As a result of distillation extraction from the fruit of Sunbigi tree, the fragrance of hydrosol is 3-Methyl-2-butenoic acid, cyclobutyl ester, Eucalyptol, L-alpha-Terpineol, 1H-Cycloprop[e]azulen-7-ol, decahydro-1 ,1,7-trimethyl-4-methylene-, [1ar-(1a.alpha.,4a.alpha.,7.beta.,7a.beta.,7b.alpha.)] were found to be many.

키워드

참고문헌

  1. 장수정, 김영희, 김명곤, 김계환, 윤세억. 2002, 순비기나무(Vitex rotundifolia L. fil.)의 부위별 정유성 분 조성. J. Korean Soc. Agric. Chem. Biotechnol. 45(2):101-107.
  2. 정연옥, 정숙진. 2017. 한국야생화식물도감-여름편 상권
  3. Davis WB. 1947. Determination of flavanones in citrus fruits. Anal Chem 19:476-478. https://doi.org/10.1021/ac60007a016
  4. Galletti GC, Russo MT. and Bocchini P. 1996. Essential oil composition of leaves and berries of Vitex agnus-castus L. from Calabria, south Italy. Rapid Commun. Mass Spectrom. 10:1345-1350 https://doi.org/10.1002/(SICI)1097-0231(199608)10:11<1345::AID-RCM631>3.0.CO;2-4
  5. Jo KJ, Yoon MY, Lee MR, Cha MR, and Park HR. 2007. The Anticancer Effect of Extracts from Vitex rotundifolia on Human Colon Carcinoma Cell Lines, J. Kerean Soc. Appl. Biol. Chem. 50(3):228-232.
  6. Kim YA, Lee JI, Kim HJ, Kong CS, Nam TJ, Seo YW. 2009. Antiproliferative Effect of Extracts, Fractions and Compound from Vitex rotundifolia on Human Cancer Cells. J. Appl. Biol. Chem. 52(4):180-186. https://doi.org/10.3839/jabc.2009.031
  7. Kim YB, Nam HJ, Park OS, Kim HJ, Kim KS, Cha JH. 2006. The Effects of Victcis Fructus & Bupleuri Radix on the Allergic rhinitis. J. Korean Oriental Med. Ophthalmology & Otolarynogology & Dermatology. 19(2):40-49.
  8. Lee MK, Kim DH, Park TS, Son JH. 2015. Antioxidant and Anti-wrinkling Effects of Extracts from Vitex trifolia L. J. Appl. Biol. Chem. 58(2):p. 125-129. https://doi.org/10.3839/jabc.2015.022
  9. Lee YS, Choi BD, Joo EY, Shin SR, Kim NW. 2009. Antioxidative Activities and Tyrosinase Inhibition Ability in Various Extracts of the Vitex rotundifolia Seeds. Korean J. Food Preserv. v.16(1):101-108
  10. Lee YS, Joo EY, Kim NW. 2008. Analysis on the Components of the Vitex rotundifolia Fruit and Stem. The Korean Society of Food Science and Nutrition. 37(2):184-189 https://doi.org/10.3746/jkfn.2008.37.2.184
  11. Mallavarapu GR, Ramesh S, Kaul PN, Bhattacharya AK, Rao BRR. 1994. Composition of essential of the leaves of Vitex negundo. Planta Med. 60:583-584. https://doi.org/10.1055/s-2006-959580
  12. Ono M, Yamamoto M, Yanaka T, Ito Y. and Nohara T. 2001. Ten new labdane-type diterpenes from the fruit of Vitex rotundifolia. Chem. Pharm. Bull. 49(1):82-86. https://doi.org/10.1248/cpb.49.82
  13. Park HJ, Kang SA, Lee JY, Cho YJ. 2012. Antioxidant Activities of Extracts from Medicinal Plants. Korean J. Food Preserv. 19(5):744-750. https://doi.org/10.11002/KJFP.2012.19.5.744
  14. Park YS, Hwang JT, Kim YS, Kim JC, Lim CH. 2012. Isolation and Identification Antifungal Compounds from Vitex trifolia L. The Korean Society Of Pesticide Science. 16(4):267-272. https://doi.org/10.7585/kjps.2012.16.4.267
  15. Singh V, Dayal R. and Vartley, JP. 1999. Volatile constituents of Vitex negundo leaves. Planta Med. 65:580-581. https://doi.org/10.1055/s-2006-960832
  16. Yeon KJ, Jung HA, Roh SS. 2013. The Effects of Vitex rotundifolia Linne fil. Extract on the Inflammatory and Allergic Reactions, 대전대학교 한의학연구소 논문집. 22(1):145-170.
  17. You JS, Chae BS, Kim DK, Cui X, Park JS, Lee JH, Kim SH, and Shin TY. 2013. Antiallergic and Anti-inflammatory Effects of the Viticis Fructus. Kor. J. Pharmacogn. 44(3):286-290.
  18. Yu JM, Kim DH, Son JH. 2015. Whitening Effects of Solvent Fractions Isolated from Vitex rotundifolia. J. Appl. Biol. Chem. 58(3):266-271. https://doi.org/10.3839/jabc.2015.042