대한의생명과학회지 (Biomedical Science Letters)

  • 제28권4호
  • /
  • Pages.307-311
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  • 2022
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
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Protective Effect of Aqueous Extract from Erigeron annuus Against Cell Death Induced by Free Radicals

  • Myeongguk, Jeong (Department of Biomedical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Hyeokjin, Kwon (Department of Biomedical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Youngdon, Ju (Department of Biomedical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Go-Eun, Choi (Department of Biomedical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Kyung-Yae, Hyun (Department of Clinical Laboratory Science, Dong-Eui University)
  • 투고 : 2022.11.21
  • 심사 : 2022.12.22
  • 발행 : 2022.12.31
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초록

The extract of EA lacks studies showing its efficacy other than that it contains caffeic acid, an active compound that has antioxidant and neuroprotective effects on nerve cells. Therefore, in this study, we attempted to determine the effectiveness of EA extraction. In this study, we performed a DPPH assay to determine the antioxidant potential of EA. And then, the cytotoxic concentration of EA in HaCaT keratinocytes was determined, and the antioxidant effect was determined by measuring the malondialdehyde (MDA). The results of DPPH, a chemical antioxidant assay, clearly demonstrated the antioxidant capacity of EA extracted with distilled water. In addition, cell-based assays provide useful information on the protective effect of EA on oxidative stress-induced apoptosis.

키워드

과제정보

This work was supported by the Busan Innovation Institute of Industry, Science & Technology Planning (BISTEP) grant funded by the Busan MetropolitanCity (Project Name: Open Laboratory Business Meeting Market Demands).

참고문헌

  1. Alia M, Ramos S, Mateos R, Granado-Serrano AB, Bravo L, Goya L. Quercetin protects human hepatoma hepg2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicology and Applied Pharmacology. 2006. 212: 110-118. https://doi.org/10.1016/j.taap.2005.07.014
  2. Ayala A, Munoz MF, Arguelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity. 2014. 2014.
  3. Chen CH, Liu TZ, Chen CH, Wong CH, Chen CH, Lu FJ, Chen SC. The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in imr-90 cells. Molecular Nutrition & Food Research. 2007. 51: 962-968. https://doi.org/10.1002/mnfr.200600230
  4. Circu ML, Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free radical biology and medicine. 2010. 48: 749-762. https://doi.org/10.1016/j.freeradbiomed.2009.12.022
  5. Han D, Chen W, Gu X, Shan R, Zou J, Liu G, Shahid M, Gao J, Han B. Cytoprotective effect of chlorogenic acid against hydrogen peroxide-induced oxidative stress in mc3t3-e1 cells through pi3k/akt-mediated nrf2/ho-1 signaling pathway. Oncotarget. 2017. 8: 14680. https://doi.org/10.18632/oncotarget.14747
  6. Jiang J, Yu S, Jiang Z, Liang C, Yu W, Li J, Du X, Wang H, Gao X, Wang X. N-acetyl-serotonin protects hepg2 cells from oxidative stress injury induced by hydrogen peroxide. Oxidative Medicine and Cellular Longevity. 2014. 2014.
  7. Lee J-S, Kim H-G, Lee H-W, Kim W-Y, Ahn Y-C, Son C-G. Pine needle extract prevents hippocampal memory impairment in acute restraint stress mouse model. Journal of Ethnopharmacology. 2017. 207: 226-236. https://doi.org/10.1016/j.jep.2017.06.024
  8. Maynard S, Schurman SH, Harboe C, de Souza-Pinto NC, Bohr VA. Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis. 2009. 30: 2-10. https://doi.org/10.1093/carcin/bgn250
  9. Olszowy M, Dawidowicz AL. Is it possible to use the dpph and abts methods for reliable estimation of antioxidant power of colored compounds? Chemical Papers. 2018. 72: 393-400. https://doi.org/10.1007/s11696-017-0288-3
  10. Ray PD, Huang B-W, Tsuji Y. Reactive oxygen species (ros) homeostasis and redox regulation in cellular signaling. Cellular Signalling. 2012. 24: 981-990.
  11. Szychta P, Zadrozny M, Lewinski A, Karbownik-Lewinska M. Increased oxidative damage to membrane lipids following surgery for breast cancer. Neuroendocrinology Letters. 2014. 35.
  12. Volpe CMO, Villar-Delfino PH, Dos Anjos PMF, Nogueira-Machado JA. Cellular death, reactive oxygen species (ros) and diabetic complications. Cell Death & Disease. 2018. 9: 1-9. https://doi.org/10.1038/s41419-017-0012-9
  13. Wellen KE, Thompson CB. Cellular metabolic stress: Considering how cells respond to nutrient excess. Molecular Cell. 2010. 40: 323-332. https://doi.org/10.1016/j.molcel.2010.10.004