대한의생명과학회지 (Biomedical Science Letters)

  • 제28권4호
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  • Pages.334-339
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  • 2022
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
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Evaluation of Loop Mediated Isothermal Amplification Based Methods for the Detection of African Swine Fever Virus from Food Waste

  • Siwon, Lee (R&D Team, LSLK Co. Ltd.) ;
  • Junhwa, Kwon (Waste-to-Energy Research Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Su Hyang, Kim (Waste-to-Energy Research Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Jin-Ho, Kim (Institute of Tissue Regeneration Engineering (ITREN), Dankook University) ;
  • Jaewon, Jung (R&D Team, LSLK Co. Ltd.) ;
  • Kyung-Jin, Lee (Department of Chemistry, College of Science and Engineering, Dankook University) ;
  • Ji-Yeon, Park (Department of Chemistry, College of Science and Engineering, Dankook University) ;
  • Taek-Kyun, Choi (KOTITI Testing & Research Institute) ;
  • Jun-Gu, Kang (Waste-to-Energy Research Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Tae Uk, Han (Waste-to-Energy Research Division, Environmental Resources Research Department, National Institute of Environmental Research)
  • 투고 : 2022.11.01
  • 심사 : 2022.12.05
  • 발행 : 2022.12.31
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초록

African swine fever virus (ASFV) is a highly contagious and lethal pathogen that poses a threat to the global pork industry. The World Organization for Animal Health (WOAH) has placed strict surveillance measures for ASFV. The possibility of long-term survival of ASFV in raw meat or undercooked pork has been reported. Accordingly, the problem of secondary infection in food waste from households or waste disposal facilities has emerged, raising the need for ASFV monitoring of food waste. However, most of the previously reported ASFV gene detection methods are focused on clinical monitoring of pigs. There are very few cases in which their application in waste has been verified. Since ASFV diagnosis requires rapid monitoring and immediate action, loop-mediated isothermal amplification (LAMP) may be suitable, but this requires conformity assessment for LAMP to be used as a diagnostic technique. In this study, six LAMP methods were evaluated, and two methods (kit and manual) were recommended for use in diagnosing ASFV in food waste.

키워드

과제정보

This work was supported by a grant from the National Institute of Environmental Research (NIER) and the Ministry of Environment (MOE) of the Republic of Korea (NIER-2020-01-01-062).

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