DOI QR코드

DOI QR Code

Post-harvest Green Pea Pod Rot Caused by Sclerotinia sclerotiorum in Korea

  • Aktaruzzaman, Md. (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Afroz, Tania (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Byung-Sup (Department of Plant Science, Gangneung-Wonju National University)
  • 투고 : 2021.12.20
  • 심사 : 2022.03.28
  • 발행 : 2022.03.31

초록

In June 2017, in Gangneung, Gangwon Province, South Korea, green pea pods exhibited post-harvest rot symptoms. The fungus was isolated from infected pea pods and cultured on potato dextrose agar for identification. The morphological characteristics were examined, sequences of the internal transcribed spacer region and the β-tubulin (βtub) gene were analyzed, and the pathogenicity was confirmed according to Koch's postulates. The morphology, phylogenetic analysis, and pathogenicity tests confirmed that Sclerotinia sclerotiorum was the causal agent. This study reports the first case of post-harvest green pea pod rot caused by S. sclerotiorum in Korea.

키워드

과제정보

This study was supported by a grant from the Rural Development Administration (project no. PJ012482), Republic of Korea. This study was also supported by the 2020 postdoctoral Fellowship Program (M. Aktaruzzaman) to the National Institute of Agricultural Sciences, RDA, Republic of Korea.

참고문헌

  1. Boland, G. J. and Hall, R. 1994. Index of plant hosts of Sclerotinia sclerotiorum. Can. J. Plant Pathol. 16: 93-108. https://doi.org/10.1080/07060669409500766
  2. Bolton, M. D., Thomma, B. P. H. J. and Nelson, B. D. 2005. Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Mol. Plant Pathol. 7: 1-16. https://doi.org/10.1111/j.1364-3703.2005.00316.x
  3. Chang, S. W. and Kim, S. K. 2003. First report of Sclerotinia rot caused by Sclerotinia sclerotiorum on some vegetable crops in Korea. Plant Pathol. J. 19: 79-84. https://doi.org/10.5423/PPJ.2003.19.2.079
  4. Farr, D. F. and Rossman, A. Y. 2021. Fungal Databases, U.S. National Fungus Collections, ARS, USDA. URL https://nt.ars-grin.gov/fungaldatabases/ [5 June 2021].
  5. Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Appl. Environ. Microbiol. 61: 1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  6. Han, K. S., Kim, J. Y., Park, J. H. and Shin, H. D. 2013. First report of Sclerotinia stem rot of anemone caused by Sclerotinia sclerotiorum in Korea. Plant Dis. 97: 997.
  7. Huang, H. C. and Kozub, G. C. 1989. A simple method for production of apothecia from sclerotia of Sclerotinia sclerotiorum. Plant Prot. Bull. Taiwan 31: 333-345.
  8. Islam, M. R., Prova, A., Akanda, A. M. and Hossain, M. M. 2020. First report of white mould caused by Sclerotinia sclerotiorum on pea in Bangladesh. J. Plant Pathol. 102: 941. https://doi.org/10.1007/s42161-020-00518-1
  9. Kim, J.-Y., Aktaruzzaman, M., Afroz, T., Hahm, Y.-I. and Kim, B.-S. 2014. The first report of postharvest stem rot of kohlrabi caused by Sclerotinia sclerotiorum in Korea. Mycobiology 42: 409-411. https://doi.org/10.5941/MYCO.2014.42.4.409
  10. Kim, W. G., Hong, S. K. and Lee, S. Y. 2006. Occurrence of Sclerotinia rot in four leguminous crop caused by Sclerotinia sclerotiorum. Plant Pathol. J. 22: 16-20. https://doi.org/10.5423/PPJ.2006.22.1.016
  11. Kim, W. G., Koo, H. M., Kim, K. H., Hyun, I. H., Hong, S. K., Cha, J. S. et al. 2009. List of Plant Diseases in Korea. 5th ed. Korean Society of Plant Pathology, Anyang, Korea. 853 pp. (In Korean)
  12. Kohn, L. M. 1979. A monographic revision of the genus Sclerotinia. Mycotaxon 9: 365-444.
  13. Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33: 1870-1874. https://doi.org/10.1093/molbev/msw054
  14. Naito, S. and Sugimoto, T. 1986. Sclerotinia stalk rot of sugar beets. Ann. Phytopathol. Soc. Jpn. 52: 217-224. https://doi.org/10.3186/jjphytopath.52.217
  15. Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
  16. Smith, E. A. and Boland, G. J. 1989. A reliable method for production and maintenance of germinated sclerotia of Sclerotinia sclerotiorum. Can. J. Plant Pathol. 11: 45-48. https://doi.org/10.1080/07060668909501145
  17. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
  18. Willetts, H. J. and Wong, J. A. L. 1980. The biology of Sclerotinia sclerotiorum, S. trifoliorum, and S. minor with emphasis on specific nomenclature. Bot. Rev. 46: 101-165. https://doi.org/10.1007/BF02860868