농업과학연구 (Korean Journal of Agricultural Science)

  • 제49권2호
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  • Pages.175-184
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  • 2022
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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Neuroprotective effect of Coreopsis lanceolata extract against hydrogen-peroxide-induced oxidative stress in PC12 cells

  • Kyung Hye Seo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyung Don Kim (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jeong-Yong Park (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Dong Hwi Kim (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Seung-Eun Lee (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Gwi Young Jang (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yun-Jeong Ji (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Ji Yeon Lee (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 투고 : 2021.11.29
  • 심사 : 2022.01.13
  • 발행 : 2022.06.01
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초록

The present study investigated the neuroprotective effects of Coreopsis lanceolate extract against hydrogen-peroxide (H2O2)-induced oxidative damage and cell death in pheochromocytoma 12 (PC12) cells. Reactive oxygen species (ROS), 2,2'-azinobis (3-ethylbebzothiazoloine-6-sulfonic acid) diammonium salt, and 1,1-diphenyl-2-picrrylhydrazyl radical scavenging activities, as well as the expression levels of proteins associated with oxidative damage and cell death were investigated. According to the results, C. lanceolate extract exhibited inhibitory activity against intracellular ROS generation and cell-damaging effects induced by hydroxyl radicals in a dose-dependent manner. Total phenolic and flavonoid contents were 22.3 mg·g-1 gallic acid equivalent and 16.2 mg·g-1 catechin equivalent, respectively. Additionally, a high-performance liquid chromatography (HPLC) assay based on the internal standard method used to detect phenolic compounds. The phenolic compounds identified in C. lanceolata extract contained (+)-catechin hydrate (5.0 ± 0.0 mg·g-1), ferulic acid (1.6 ± 0.0 mg·g-1), chlorogenic acid (1.5 ± 0.0 mg·g-1), caffeic acid (1.2 ± 0.0 mg·g-1), naringin (0.9 ± 0.0 mg·g-1), and p-coumaric acid (0.5 ± 0.0 mg·g-1). C. lanceolata extract attenuated pro-apoptotic Bax expression levels and enhanced the expression levels of anti-apoptotic Bcl-2, caspase-3, and caspase-9 proteins. Therefore, C. lanceolata is a potential source of materials with neuroprotective properties against neurodegenerative disorders, such as Alzheimer's and Parkinson's diseases.

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과제정보

본 연구는 원예특작시험연구사업(사업번호: PJ01415801)의 지원에 의해 이루어진 결과로 이에 감사드립니다.

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