Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Genetic variation of the endangered species Halenia coreana (Gentianaceae)

  • YUN, Narae (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • OH, Sang-Hun (Department of Biology, Daejeon University)
  • Received : 2022.02.08
  • Accepted : 2022.03.15
  • Published : 2022.03.31
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Abstract

Halenia coreana is an endangered, endemic species that is distributed in only a few locations in Korea, such as Mts. Hwaaksan and Daeamsan. It has been recently segregated from H. corniculata, broadly distributed in cold temperate regions that include northern Japan, the Russian Far East, northeastern China, Mongolia, and eastern Europe, where population sizes are usually large. To examine the genetic diversity of H. coreana and evaluate the level of genetic differentiation of the species compared with that of H. corniculata, we surveyed 183 candidate simple sequence repeats (SSR) motif markers for H. coreana and H. corniculata from sequence data of amplified fragments of a specific length in the genome. A total of 17 genomic-SSR markers were selected to examine the levels of genetic diversity and differentiation using 17 samples of H. coreana and 60 samples of three populations of H. corniculata. The results here suggest that the genetic diversity of H. coreana is very low with a high frequency of inbreeding within its population. We found that H. coreana is genetically differentiated from H. corniculata, supporting the recognition of the geographically isolated H. coreana as a distinct species.

Keywords

Acknowledgement

We are grateful to Jin-Oh Hyun for providing the Russian samples, Young-Dong Kim and Bo-Yun Kim for information about the sampling sites, and Yun Gyeong Choi and Seung-Hyun Hwang for their help in various stages of this research. We also thank the staff at KB for allowing us to examine their herbarium specimens. This work was supported by research grants from the National Institute of Biological Resources of Korea (NIBR-201703201) and the National Research Foundation of Korea (NRF 2020R1I1A3068464).

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