한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제37권4호
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  • Pages.246-254
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  • 2022
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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Enhancement of anti-inflammatory and anti-tumorigenic properties of 3D-spheroid formed mesenchymal stem cells derived from rheumatoid arthritis joints

  • Seung-Chan, Lee (Apures, Inc., Central Research Center) ;
  • Chae-Yeon, Hong (College of Veterinary Medicine, Gyeongsang National University) ;
  • Yong-Ho, Choe (College of Veterinary Medicine, Gyeongsang National University) ;
  • Tae-Seok, Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Won-Jae, Lee (College of Veterinary Medicine, Kyungpook National University) ;
  • Gyu-Jin, Rho (College of Veterinary Medicine, Gyeongsang National University) ;
  • Sung-Lim, Lee (College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2022.11.26
  • 심사 : 2022.12.05
  • 발행 : 2022.12.31
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초록

Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and anti-inflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient's synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RA-hMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

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과제정보

This study was supported by a grant from the National Research Foundation (NRF) of Korea, funded by the government of the Republic of Korea (grant no. NRF-2021R1A2C1007054).

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