한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제37권4호
  • /
  • Pages.226-230
  • /
  • 2022
  • /
  • 2671-4639(pISSN)
  • /
  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Embryo transfer of dorper breed to Mongolian sheep

  • Chuluunbayar, Uuganbayar (School of Animal Science and Biotechnology, Mongolian University of Life Sciences) ;
  • Tsolmonbaatar, Boldsaikhan (Livestock Gene Bank) ;
  • Byambasaikhan, Danzan-Osor (Animal Science Sector, Research Institute of Animal Husbandry, Mongolian University of Life Sciences) ;
  • Ho-Jun, Lee (School of Animal Life Convergence Science, Hankyong National University) ;
  • Sang-Hwan, Kim (School of Animal Life Convergence Science, Hankyong National University) ;
  • Enkhbolor, Barsuren (School of Animal Science and Biotechnology, Mongolian University of Life Sciences)
  • 투고 : 2022.09.08
  • 심사 : 2022.10.16
  • 발행 : 2022.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The sheep can be reproduced by natural mating as well as applied reproductive biotechnology, embryo transfer (ET). However, this method in sheep is influenced by several factors such as season, photoperiod, latitude, temperature, nutrition, and breed. In addition, there is still less research on assisted reproductive technologies in small ruminants, compared to other livestock species such as cattle and pigs. Because there has been a need for an optimization and a continuous improvement of ET techniques in small ruminants. the main objective of this study was to evaluate the conception rate obtained after ET in Mongolian sheep (Dorper breed). After embryo recover, code 1 and 2 embryos (morula or blastocyst stage) for ET in the present study were 63% (63/100) and 24% (24/100), respectively. Then Each single embryo was transferred to a synchronized recipient who prepared by estrous synchronization protocol with fluorogestone acetate-cloprostenol sodium. The results demonstrated that an average conception rate and lambing rate was 35.6% (31/87) and 33.3% (29/87), respectively. Further study is still necessary, but these results indicated that single embryo of Mongolian sheep with the present protocol was enough to conducting ET when the genetically superior sheep were necessary to be expanded.

키워드

과제정보

This paper and the research behind it would not have been possible without the exceptional support of Tumen Shuvuut LLC and researchers.

참고문헌

  1. Ali J and Shelton JN. 1993. Successful vitrification of day-6 sheep embryos. J. Reprod. Fertil. 99:65-70. https://doi.org/10.1530/jrf.0.0990065
  2. Brown BW and Radziewic T. 1998. Production of sheep embryos in vitro and development of progeny following single and twin embryo transfers. Theriogenology 49:1525-1536. https://doi.org/10.1016/S0093-691X(98)00098-3
  3. Ciornei SG, Drugociu D, Ciornei L, Rosca P. 2022. Ovarian response to P4-PGF-FSH treatment in Suffolk sheep and P4- PGF-PMSG synchronization in cross-bred ewes, for IVD and ET protocol. Vet. Med. Sci. 8:726-734. https://doi.org/10.1002/vms3.705
  4. Coonrod SA, Coren BR, McBride BL, Bowen MJ, Kraemer DC. 1986. Successful non-surgical collection of ovine embryos. Theriogenology 25:149.
  5. Gordon I. 1997. Controlled Reproduction in Sheep and Goats. CAB International, Wallingford, pp. 273.
  6. Ledda S, Bogliolo L, Leoni G, Loi P, Cappai P, Naitana S. 1995. Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. Reprod. Nutr. Dev. 35:629-637. https://doi.org/10.1051/rnd:19950603
  7. Paramio MT and Izquierdo D. 2014. Current status of in vitro embryo production in sheep and goats. Reprod. Domest. Anim. 49 Suppl 4:37-48. https://doi.org/10.1111/rda.12334
  8. Rizzo F, Vitale N, Ballardini M, Borromeo V, Luzzago C, Chiavacci L, Mandola ML. 2016. Q fever seroprevalence and risk factors in sheep and goats in northwest Italy. Prev. Vet. Med. 130:10-17. https://doi.org/10.1016/j.prevetmed.2016.05.014
  9. Schiewe MC, Rall WF, Stuart LD, Wildt DE. 1991. Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos. Theriogenology 36:279-293. https://doi.org/10.1016/0093-691X(91)90386-R
  10. Shi JM, Yi JY, Tian XZ, Wang F, Lian ZX, Han HB, Fu JC, Lv WF, Liu GS. 2015. Effects of seasonal changes on the ovulation rate and embryo quality in superovulated Black Suffolk ewes. Neuro Endocrinol. Lett. 36:330-336.
  11. Szell A, Zhang J, Hudson R. 1990. Rapid cryopreservation of sheep embryos by direct transfer into liquid nitrogen vapour at -180 degrees C. Reprod. Fertil. Dev. 2:613-618. https://doi.org/10.1071/RD9900613
  12. Vajta G. 2000. Vitrification of the oocytes and embryos of domestic animals. Anim. Reprod. Sci. 60-61:357-364. https://doi.org/10.1016/S0378-4320(00)00097-X