The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide

  • Moghrovyan, Armenuhi (Department of Pharmacognosy, Yerevan State Medical University after M. Heratsi) ;
  • Parseghyan, Lilya (Orbeli Institute of Physiology, Laboratory of Physiologically Active Substances Investigations) ;
  • Sevoyan, Gohar (Orbeli Institute of Physiology, Laboratory of Tissue Engineering) ;
  • Darbinyan, Anna (Orbeli Institute of Physiology, Laboratory of Physiologically Active Substances Investigations) ;
  • Sahakyan, Naira (Yerevan State University, Research Institute of Biology, Faculty of Biology) ;
  • Gaboyan, Monica (Yerevan State Medical University after M. Heratsi, Faculty of Pharmacy) ;
  • Karabekian, Zaruhi (Orbeli Institute of Physiology, Laboratory of Tissue Engineering) ;
  • Voskanyan, Armen (Orbeli Institute of Physiology, Laboratory of Physiologically Active Substances Investigations)
  • Received : 2021.11.23
  • Accepted : 2022.02.02
  • Published : 2022.04.01
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Abstract

Background: Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation. Methods: For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used. Results: The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 µL/mL OVA essential oil in media. Conclusions: The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations.

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