DOI QR코드

DOI QR Code

Occurence of Powdery Mildew Caused by Podosphaera xanthii on Vigna nakashimae in Korea

Podosphaera xanthii에 의한 좀돌팥 흰가루병 발생

  • Kyung-Gu, Min (Department of Plant Resources, Kongju National University) ;
  • Tae-Min, Park (Department of Plant Resources, Kongju National University) ;
  • Youn-Jin, Park (Kongju National University Legumes Green Manure Resource Center) ;
  • Myoung-Jun, Jang (Department of Plant Resources, Kongju National University)
  • 민경구 (공주대학교 식물자원학과) ;
  • 박태민 (공주대학교 식물자원학과) ;
  • 박윤진 (두과녹비자원연구센터) ;
  • 장명준 (공주대학교 식물자원학과)
  • Received : 2022.11.23
  • Accepted : 2022.12.22
  • Published : 2022.12.31

Abstract

In October 2022, common Asian cowpea (Vigna nakashimae) powdery mildew disease was investigated at Yesan-gun, Chungcheongnam-do, Korea (36°40'12.7"N,126°51'36.4"E). The disease mainly occurred on the front of the leaves, and weak powdery mildew disease occurred on the back side of leaf. The chasmothecia was identified on the back side of the leaf. Analysis of the internal transcribed spacer rDNA sequence and 28S large subunit sequence, along with the morphological characteristics of the anamorph and teleomorph of the causal fungus identified as Podosphaera xanthii. This is the first time in Korea to report common Asian cowpea (Vigna nakashimae) powdery mildew disease caused by Podospaera xanthii.

2022년 10월 충청남도 예산군(36°40'12.7"N, 126°51'36.4"E)에서 좀돌팥 흰가루병을 조사하였다. 병징은 주로 잎의 앞면에 발생하였으며 뒷면에는 흰가루병이 약하게 발병하였다. 흰가루병의 자낭구는 잎의 뒷면에서 확인하였다. 흰가루병균 무성세대 및 유성세대의 형태적 특징과 더불어 internal transcribed spacer rDNA, 28S large subunit 염기서열을 계통분석한 결과, 흰가루병균은 Podosphaera xanthii로 동정되었다. 이는 한국에서 Podosphaera xanthii에 의한 좀돌팥 흰가루병을 처음으로 보고한다.

Keywords

Acknowledgement

This research was supported by Kongju National University Academic Research Support Project, 2022.

References

  1. Braun, U. and Cook, R. T. 2012. Taxonomic Manual of the Erysiphales (powdery mildews). CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands. 707
  2. Farr, D. F. and Rossman, A. Y. 2022. Fungal Databases, Systematic Mycology & Microbiology Laboratory, ARS, USDA. URL http://nt.arsgrin.gov/fungaldatabases/ [23 November 2022].
  3. Katoh, K. and Toh, H. 2010. Parallelization of the MAFFT multiple sequence alignment program. Bioinformatics 26: 1899-1900. https://doi.org/10.1093/bioinformatics/btq224
  4. Khodaparast, S. A., Takamatsu, S. and Hedjaroude, G. A. 2005. Phylogenetic analysis of Iranian powdery mildew fungi using nucleotide sequences of 28S ribosomal DNA. J. Agric. Sci. Technol. 7: 49-58.
  5. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35: 1547-1549. https://doi.org/10.1093/molbev/msy096
  6. Lee, J., Baek, H.-J., Yoon, M.-S., Cho, G.-T. and Kim, C.-Y. 2006. Identification and use of correct scientific names of Vigna angularis var. nipponensis (Ohwi) Ohwi & Ohashi and V. nakashimae (Ohwi) Ohwi & Ohashi in Korea. Korean J. Plant Taxon. 36: 217-225. https://doi.org/10.11110/kjpt.2006.36.3.217
  7. Lee, S. Y., Hwang, S. J. and Lee, S. B. 2002. Occurrence of powdery mildew on mung bean (Vigna radiatus L.) caused by Sphaerotheca phaseoli. Res. Plant Dis. 8: 166-170. https://doi.org/10.5423/RPD.2002.8.3.166
  8. Nam, M. H., Jeon, Y. N., Lee, H. C., Lee, H. D. and Kang, H. K. 2012. Comparative analysis between healthy and powdery mildew-infected plants of strawberry cultivar Seolhyang. Res. Plant Dis. 18: 80-85. https://doi.org/10.5423/RPD.2012.18.2.080
  9. Somta, P., Kaga, A., Tomooka, N., Kashiwaba, K., Isemura, T., Chaitieng, B. et al. 2006. Development of an interspecific Vigna linkage map between Vigna umbellata (Thunb.) Ohwi & Ohashi and V. nakashimae (Ohwi) Ohwi & Ohashi and its use in analysis of bruchid resistance and comparative genomics. Plant Breed. 125: 77-84. https://doi.org/10.1111/j.1439-0523.2006.01123.x
  10. The Korean Society of Plant Pathology. 2022. List of Plant Diseases in Korea. 6th ed. Korean Society of Plant Pathology, Seoul, Korea. 630
  11. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, New York, USA.
  12. Yoon, M. S., Lee, J., Kim, C. Y. and Baek, H. J. 2007. Genetic relationships among cultivated and wild Vigna angularis (Willd.) Ohwi et Ohashi and relatives from Korea based on AFLP markers. Genet. Resour. Crop Evol. 54: 875-883. https://doi.org/10.1007/s10722-006-9156-7