Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Occurrence of Viruses and Viroids in Chrysanthemum Plants (Dendranthema morifolium) Cultivated in Yesan-gun, Chungcheongnam-do in Korea

충남 예산 지역의 국화에서 바이러스 및 바이로이드 병들의 발생 현황

  • Yoon Hyun, Bang (Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University) ;
  • Eun Gyeong, Song (Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University) ;
  • Younghye, Lee (Flower Research Institute, Chungcheongnam-do, Agricultural Research & Extension Services) ;
  • Ki Hyun, Ryu (Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University)
  • 방윤현 (서울여자대학교 자연과학대학원예생명조경학과 식물바이러스은행) ;
  • 송은경 (서울여자대학교 자연과학대학원예생명조경학과 식물바이러스은행) ;
  • 이영혜 (충청남도 농업기술원화훼연구소) ;
  • 류기현 (서울여자대학교 자연과학대학원예생명조경학과 식물바이러스은행)
  • Received : 2022.11.25
  • Accepted : 2022.12.19
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Chrysanthemum plants are one of the most economically important plants in South Korea. Both virus and viroid can cause diseases and economic damage to the plants. In this study, we investigated the detection of seven viruses and two viroids in 350 chrysanthemum plants cultivated in Yesan-gun, Chungcheongnam-do. Two viruses, chrysanthemum virus B (CVB) and tomato aspermy virus (TAV), and two viroids, chrysanthemum chlorotic mottle viroid (CChMVd) and chrysanthemum stunt viroid (CSVd), were detected in this study. The two viruses were detected in six samples and one sample, respectively. The two viroids were detected in 97 samples and 21 samples, respectively. The nucleotide sequences of the CVB-CN-Y, TAV-CN-Y, CChMVd-CN-Y, and CSVd-CN-Y obtained in this study showed 83.7-86.9%, 99.2-100.0%, 94.4-99.5%, and 95.7-99.7% identity, respectively, compared to their other strains/isolates. The CVB-CN-Y and TAV-CN-Y showed the greatest nucleotide sequence homology to CVB-GS1 and three TAV isolates (TAV-V, TAV-P, and TAV-ChJ), respectively. The CChMVd-CN-Y and CSVd-CN-Y showed the greatest nucleotide sequence homology to CChMVd-Horst and four CSVd isolates (Au1.1, K4pop, Sagae, and Tochigi), respectively. This study is the report on the infection rate of viruses and viroids in chrysanthemum plants cultivated in Yesan-gun in 2021.

국화(Dendranthema morifolium)는 한국에서 경제적으로 중요한 화훼식물에 포함되고, 바이러스와 바이로이드 병들에 의해 경제적 피해를 받고 있다. 본 연구에서는 충청남도 예산군에서 재배된 국화 350개체를 대상으로 7종의 바이러스들과 2종의 바이로이드들(Chrysanthemum chlorotic mottle viroid, CChMVd; Chrysanthemum stunt viroid, CSVd; Cucumber mosaic virus, CMV; Chrysanthemum virus B, CVB; Chrysanthemum stem necrosis orthotospovirus, CSNV; Impatiens necrotic spot orthotospovirus, INSV; Potato virus X, PVX; Tomato aspermy virus, TAV; Tomato spotted wilt orthotospovirus, TSWV)을 검정하였다. 그 결과 2종의 바이러스들(CVB-CN-Y, TAV-CN-Y)과 2종의 바이로이드들(CChMVd-CN-Y, CSVd-CN-Y)이 검정되었다. CVBCN-Y는 6개의 국화 식물체들에서 검정되었고, TAV-CN-Y는 한개가 국화 식물체에서만 검정되었다. CChMVd-CN-Y는 97개의 국화 식물체들에서 검정되었으며, CSVd-CN-Y는 21개의 국화 식물체들에서 검정되었다. CVB-CN-Y는 CVB-GS1과 86.9% 염기서열 상동성을 보였으며, TAV-CN-Y는 3개의 분리주들(TAV-Chj, TAV-P, TAV-V)과 100% 염기서열이 일치하였다. CChMVd-CN-Y는 CChMVd-Horst와 99.5% 염기서열 상동성을 보였으며, CSVd-CN-Y는 4개의 분리주들(Au1.1, K4pop, Sagae, Tochigi)과 99.7% 염기서열 상동성을 보였다. 본 연구는 2021년 예산군에서 재배된 국화들을 대상으로 바이러스 및 바이로이드들을 검정하고 그들의 감염률에 관한 보고서이다.

Keywords

Acknowledgement

This study was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Crop Viruses and Pests Response Industry Technology Development Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (grant number 120084-3). This study was also supported by a sabbatical year research grant from Seoul Women's University (2021). Yoon Hyun Bang and Eun Gyeong Song contributed equally to this study and should be considered as cofirst authors.

References

  1. Ashwathappa, K. V., Venkataravanappa, V., Reddy, C. N. and Reddy, M. K. 2020. Association of Tomato leaf curl New Delhi virus with mosaic and leaf curl disease of chrysanthemum and its whitefly cryptic species. Indian Phytopathol. 73: 533-542. https://doi.org/10.1007/s42360-020-00214-1
  2. Cho, W. K., Jo, Y., Jo, K.-M. and Kim, K.-H. 2013. A current overview of two viroids that infect chrysanthemums: chrysanthemum strunt viroid and chrysanthemum chlorotic mottle viroid. Viruses 5: 1099-1113. https://doi.org/10.3390/v5041099
  3. Chung, B. N., Choi, G. S. and Choi, Y. M. 1999. Identification of tomato aspermy virus (TAV) and chrysanthemum virus B (CVB) from Dendranthema indicum in Korea. Plant Pathol. J. 15: 119-123.
  4. Chung, B. N., Choi, G. S., Kim, H. R. and Kim, J. S. 2001. Chrysanthemum stunt viroid in Dendranthema grandiflorum. Plant Pathol. J. 17: 194-200.
  5. Chung, B. N., Huh, E. J. and Kim, J. S. 2006a. Effect of temperature on the concentration of chrysanthemum stunt viroid in CSVd-infected chrysanthemum. Plant Pathol. J. 22: 152-154. https://doi.org/10.5423/PPJ.2006.22.2.152
  6. Chung, B. N., Kim, D. C., Kim, J. S. and Cho, J. D. 2006b. Occurrence of chrysanthemum chlorotic mottle viroid in chrysanthemum (Dendranthema grandiflorum) in Korea. Plant Pathol. J. 22: 334-338. https://doi.org/10.5423/PPJ.2006.22.4.334
  7. Chung, B. N., Lim, J. H., Choi, S. Y., Kim, J. S. and Lee, E. J. 2005. Occurrence of chrysanthemum stunt viroid in chrysanthemum in Korea. Plant Pathol. J. 21: 377-382. https://doi.org/10.5423/PPJ.2005.21.4.377
  8. Chung, B. N. and Pak, H. S. 2008. Seed transmission of chrysanthemum stunt viroid in chrysanthemum. Plant Pathol. J. 24: 31-35. https://doi.org/10.5423/PPJ.2008.24.1.031
  9. Chung, B. N., Pak, H. S., Jung, J. A. and Kim, J. S. 2006c. Occurrence of tomato spotted wilt virus in chrysanthemum (Dendranthema grandiflorum) in Korea. Plant Pathol. J. 22: 230-234. https://doi.org/10.5423/PPJ.2006.22.3.230
  10. Ebata, M. Matsushita, Y., Morimoto, M. and Mochizuki, T. 2019. Distribution of chrysanthemum chlorotic mottle viroid in shoot meristem and flower buds of chrysanthemum. Eur. J. Plant Pathol. 154: 555-563.
  11. Guan, Z., Wu, D., Song, A., Chen, F., Chen, S. and Fang, W. 2017. A highly sensitive method for the detection of chrysanthemum virus B. Electron. J. Biotechnol. 26: 64-68. https://doi.org/10.1016/j.ejbt.2017.01.003
  12. Gupta, R., Gupta, A., Jain, S., Singh, D. and Verma, N. 2021. Chrysanthemum production, viral diseases and their management. In: Virus Diseases of Ornamental Plants: Characterization, Identification, Diagnosis and Management, eds. by S. K. Raj, R. K. Gaur and Z. Yin, pp. 261-275. Springer, Singapore.
  13. Khan, A. U., Choudhury, M. A. R., Khan, A. U., Khanal, S. and Maukeeb, A. R. M. 2021. Chrysanthemum production in Bangladesh: significance the insect pests and disease management: a review. J. Multidiscip. Appl. Nat. Sci. 1: 25-35. https://doi.org/10.47352/jmans.v1i1.10
  14. Kim, B. M., Kim, S. Y. and Lim, J. H. 2021. Analysis of domestic and overseas marketability for revitalization of exports of cut flowers and Chrysanthemums produced in Korea. Flower Res. J. 29: 29-41. https://doi.org/10.11623/frj.2021.29.1.04
  15. Kim, S. J., Lee, S. K. and Kim, K. S. 2012. Current research trend of postharvest technology for chrysanthemum. Korean J. Plant Res. 25: 156-168. https://doi.org/10.7732/kjpr.2012.25.1.156
  16. Kim, W.-S., Haj-Ahmad, Y., Stobbs, L.W. and Greig, N. 2015. Evaluation of viroid extraction methods and application of a one-step reverse transcription real-time polymerase chain reaction assay (RT-qPCR) for the rapid detection of chrysanthemum stunt viroid (CSVd) infection. Can. J. Plant Pathol. 37: 221-229. https://doi.org/10.1080/07060661.2015.1012742
  17. Kondo, T., Yamashita, K. and Sugiyama, S. 2011. First report of impatiens necrotic spot virus infecting chrysanthemum (Chrysanthemum morifolium) in Japan. J. Gen. Plant Pathol. 77: 263-265. https://doi.org/10.1007/s10327-011-0317-y
  18. Kumar, S., Khan, M. S., Raj, S. K. and Sharma, A. K. 2009. Elimination of mixed infection of cucumber mosaic and tomato aspermy virus from Chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture. Sci. Hortic. 119: 108-112. https://doi.org/10.1016/j.scienta.2008.07.017
  19. Kwak, H.-R., Kim, J.-G., Kim, J.-E., Byun, H.-S., Choi, H.-S., Wintermantel, W. M. et al. 2021. First report of tomato aspermy virus in Chrysanthemum zawadskii var. latilobum in Korea. J. Plant Pathol. 103: 1005-1006.
  20. Kwon, Y. E., Song, E. G., Choi, S. H. and Ryu, K. H. 2020. Genetic differences between Korean and American isolates of petunia vein clearing virus. Virus Genes 56: 78-86. https://doi.org/10.1007/s11262-019-01711-w
  21. Lewis, T. 1997. Pest thrips in perspective. In: Thrips as Crop Pests, ed. by T. Lewis, pp. 1-13. CAB International, Wallingford, UK.
  22. Liu, J.-J., Goh, C.-J., Loh, C.-S., Liu, P. and Pua, E.-C. 1998. A method for isolation of total RNA from fruit tissue of banana. Plant Mol. Biol. Rep. 16: 87.
  23. Liu, X. L., Wei, Q., Hong, B. and Zhao, X. T. 2014. First report of potato virus Y strain N-Wilga infecting chrysanthemum in China. Plant Dis. 98:1589.
  24. Ministry of Agriculture, Food and Rural Affairs. 2021. Annual Report of Floriculture. MAFRA Press, Sejong, Korea. 17 pp.
  25. Nassar, E. A., El-Dougdoug, K. A., Osman, M. E., Dawoud, R. A. and Kinawy, A. H. 2012. Characterization and elimination of a TMV isolate infecting Chrysanthemum plants in Egypt. Int. J. Virol. 8: 14-26. https://doi.org/10.3923/ijv.2012.14.26
  26. Niu, E. B., Chen, L. J. and Niu, Y. B. 2015. First report of zucchini yellow mosaic virus in chrysanthemum. Plant Dis. 99: 1289.
  27. Ohkawa, A., Yamada, M., Sayama, H., Sugiyama, N., Okuda, S. and Natsuaki, T. 2007. Complete nucleotide sequence of a Japanese isolate of chrysanthemum virus B (genus Carlavirus). Arch. Virol. 152: 2253-2258. https://doi.org/10.1007/s00705-007-1039-5
  28. Ram, R. Verma, N., Singh, A. K., Singh, L., Hallan, V. and Zaidi, A. A. 2005. Indexing and production of virus-free chrysanthemums. Biol. Plant. 49: 149-152. https://doi.org/10.1007/s10535-005-0152-0
  29. Ram, R. Verma, N., Singh, A. K. and Zaidi, A. A. 2009. Virus-free chrysanthemum: production and quality management. Arch. Phytopathol. Plant Prot. 42: 940-949. https://doi.org/10.1080/03235400701541396
  30. Song, A., You, Y., Chen, F., Li, P., Jiang, J. and Chen, S. 2012. A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum. Lett. Appl. Microbiol. 56: 8-13. https://doi.org/10.1111/lam.12007
  31. Song, E. G., Lee, H. S. and Ryu, K. H. 2016. Occurrence of hydrangea ringspot virus and hydrangea chlorotic mottle virus in hydrangea plants in South Korea. J. Gen. Plant Pathol. 82: 281-285. https://doi.org/10.1007/s10327-016-0670-y
  32. Supakitthanakorn, S., Vichittragoontavorn, K., Kunasakdakul, K. and Ruangwong, O.-U. 2022a. Development of the colorimetric loop-mediated isothermal amplification technique for rapid and sensitive detection of chrysanthemum stunt viroid in chrysanthemum. J. Plant Prot. Res. 62: 272-280.
  33. Supakitthanakorn, S., Vichittragoontavorn, K., Kunasakdakul, K. and Ruangwong, O.-U. 2022b. Phylogenetic analysis and molecular characterization of chrysanthemum chlorotic mottle viroid and chrysanthemum stunt viroid from chrysanthemum in Thailand. J. Phytopathol. 170: 700-710. https://doi.org/10.1111/jph.13134
  34. Supakitthanakorn, S., Vichittragoontavorn, K., Sunpapao, A., Kunasakdakul, K., Thapanapongworakul, P. and Ruangwong, O.-U. 2022c. Tobacco mosaic virus infection of chrysanthemums in Thailand: development of colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) technique for sensitive and rapid detection. Plants 11: 1788.
  35. Trolinger, J. C., McGovern, R. J., Elmer, W. H., Rechcigl, N. A. and Shoemaker, C. M. 2017. Diseases of chrysanthemum. In: Handbook of Florists' Crops Diseases, eds. by R. J. McGovern and W. H. Elmwe, pp. 1-66. Springer International Publishing AG, New York, USA.
  36. Yoon, J. Y., Choi, G. S. and Choi, S. K. 2017. First report of chrysanthemum stem necrotic virus on Chrysanthemum morifolium in Korea. Plant Dis. 101: 264.
  37. Yoon, J.-Y., Yoon, J.-B., Seo, M.-H., Choi, S.-K., Cho, I.-S., Chung, B.-N. et al. 2020. Application of multiplex RT-PCR for simultaneous identification of tomato spotted wilt virus and thrips species in an individual thrips on chrysanthemum. Res. Plant Dis. 26: 264-271. (in Korean) https://doi.org/10.5423/RPD.2020.26.4.264
  38. Verma, N., Kumar, K., Kulshrestha, S., Raikhy, G., Hallan, V., Ram, R. et al. 2009. Molecular studies on tomato aspermy virus isolates infecting chrysanthemums. Arch. Phytopathol. Plant Prot. 42: 99-111. https://doi.org/10.1080/03235400600951779
  39. Verma, N., Ram, R., Hallan, V., Kumar, K. and Zaidi, A. A. 2004. Production of cucumber mosaic virus-free chrysanthemums by meristem tip culture. Crop Prot. 23: 469-473. https://doi.org/10.1016/j.cropro.2003.08.021
  40. Zhao, X., Liu, X., Ge, B., Li, M. and Hong, B. 2015. A multiplex RT-PCR for simultaneous detection and identification of five viruses and two viroids infecting chrysanthemum. Arch Virol. 160: 1145-1152.  https://doi.org/10.1007/s00705-015-2360-z