대한수의학회지 (Korean Journal of Veterinary Research)

  • 제62권1호
  • /
  • Pages.10.1-10.9
  • /
  • 2022
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

Isolation and molecular characterization of feline panleukopenia viruses from Korean cats

  • Yang, Dong-Kun (Viral Disease Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Park, Yu-Ri (Viral Disease Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Park, Yeseul (Viral Disease Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • An, Sungjun (Viral Disease Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Choi, Sung-Suk (Viral Disease Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Park, Jungwon (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Hyun, Bang-Hun (Viral Disease Division, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs)
  • 투고 : 2021.12.02
  • 심사 : 2022.02.04
  • 발행 : 2022.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological and genetic features of South Korean FPVs remain unclear. We characterized molecularly South Korean FPV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FPV from 60 organ homogenates. The isolates were confirmed to be FPVs via analyses of cytopathic effects, immunofluorescence studies, electron microscopy, and polymerase chain reaction. Viral genetic analyses used the full VP2 sequences. Eight isolates propagated in CRFK cells were confirmed to be FPVs. All isolates yielded viral titers ranging from 104.5 to 106.0 TCID50/mL 5 days after inoculation into CRFK cells and exhibited hemagglutination titers ranging from 27 to 212 (using pig erythrocytes). The Korean FPV isolates grew well in cat cells such as CRFK and Fcwf-4 cells. The FPV isolates were most similar to the KS42 strain isolated from a Korean cat in 2008. The FPV isolates will serve as useful antigens in future sero-epidemiological studies and will aid in the development of diagnostic tools.

키워드

과제정보

This study was supported financially by a grant (B-1543083-2020-22-01) from the Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Korea.

참고문헌

  1. Balboni A, Bassi F, De Arcangeli S, Zobba R, Dedola C, Alberti A, Battilani M. Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats. BMC Vet Res 2018;14:41. https://doi.org/10.1186/s12917-018-1356-9
  2. Wang X, Zhang J, Huo S, Zhang Y, Wu F, Cui D, Yu H, Zhong F. Development of a monoclonal antibody against canine parvovirus NS1 protein and investigation of NS1 dynamics and localization in CPV-infected cells. Protein Expr Purif 2020; 174:105682. https://doi.org/10.1016/j.pep.2020.105682
  3. Saxena L, Kumar GR, Saxena S, Chaturvedi U, Sahoo AP, Singh LV, Santra L, Palia SK, Desai GS, Tiwari AK. Apoptosis induced by NS1 gene of Canine Parvovirus-2 is caspase dependent and p53 independent. Virus Res 2013;173:426-430. https://doi.org/10.1016/j.virusres.2013.01.020
  4. Wang D, Yuan W, Davis I, Parrish CR. Nonstructural protein-2 and the replication of canine parvovirus. Virology 1998;240:273-281. https://doi.org/10.1006/viro.1997.8946
  5. Vihinen-Ranta M, Wang D, Weichert WS, Parrish CR. The VP1 N-terminal sequence of canine parvovirus affects nuclear transport of capsids and efficient cell infection. J Virol 2002; 76:1884-1891. https://doi.org/10.1128/JVI.76.4.1884-1891.2002
  6. Chang D, Liu Y, Chen Y, Hu X, Burov A, Puzyr A, Bondar V, Yao L. Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system. BMC Vet Res 2020;16:202. https://doi.org/10.1186/s12917-020-02422-3
  7. Su WJ, Shen WD, Li B, Wu Y, Gao G, Wang WB. A novel way to purify recombinant baculoviruses by using bacmid. Biosci Rep 2009;29:71-75. https://doi.org/10.1042/BSR20080074
  8. Chung HC, Kim SJ, Nguyen VG, Shin S, Kim JY, Lim SK, Park YH, Park B. New genotype classification and molecular characterization of canine and feline parvoviruses. J Vet Sci 2020;21:e43. https://doi.org/10.4142/jvs.2020.21.e43
  9. Yang S, Wang S, Feng H, Zeng L, Xia Z, Zhang R, Zou X, Wang C, Liu Q, Xia X. Isolation and characterization of feline panleukopenia virus from a diarrheic monkey. Vet Microbiol 2010;143:155-159. https://doi.org/10.1016/j.vetmic.2009.11.023
  10. Parrish CR. Pathogenesis of feline panleukopenia virus and canine parvovirus. Baillieres Clin Haematol 1995;8:57-71. https://doi.org/10.1016/S0950-3536(05)80232-X
  11. Jacobson LS, Janke KJ, Giacinti J, Weese JS. Diagnostic testing for feline panleukopenia in a shelter setting: a prospective, observational study. J Feline Med Surg 2021;23:1192-1199. https://doi.org/10.1177/1098612X211005301
  12. Yang DK, Park Y, Park YR, Yoo JY, An S, Park J, Hyun BH. Expression of the VP2 protein of feline panleukopenia virus in insect cells and use thereof in a hemagglutination inhibition assay. Korean J Vet Res 2021;61: e19. https://doi.org/10.14405/kjvr.2021.61.e19
  13. Abd-Eldaim M, Beall MJ, Kennedy MA. Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus. Vet Ther 2009;10:E1-E6.
  14. An DJ, Jeong W, Jeoung HY, Yoon SH, Kim HJ, Park JY, Park BK. Phylogenetic analysis of feline panleukopenia virus (FPLV) strains in Korean cats. Res Vet Sci 2011;90:163-167. https://doi.org/10.1016/j.rvsc.2010.05.010
  15. Kim YJ, Yoon SW, Jang JH, Jeong DG, Lee BJ, Kim HK. Genetic characterization of feline parvovirus isolate fe-p2 in Korean cat and serological evidence on its infection in wild leopard cat and Asian badger. Front Vet Sci 2021;8:650866. https://doi.org/10.3389/fvets.2021.650866
  16. Stuetzer B, Hartmann K. Feline parvovirus infection and associated diseases. Vet J 2014;201:150-155. https://doi.org/10.1016/j.tvjl.2014.05.027
  17. Yang DK, Park YR, You JY, Choi SS, Park Y, An S, Park J, Kim HJ, Kim J, Kim HH, Hyun BH. Biological and molecular characterization of feline caliciviruses isolated from cats in South Korea. Korean J Vet Res 2020;60:195-202. https://doi.org/10.14405/KJVR.2020.60.4.195
  18. Parker JS, Murphy WJ, Wang D, O'Brien SJ, Parrish CR. Canine and feline parvoviruses can use human or feline transferrin receptors to bind, enter, and infect cells. J Virol 2001;75: 3896-3902. https://doi.org/10.1128/JVI.75.8.3896-3902.2001
  19. Truyen U, Parrish CR. Canine and feline host ranges of canine parvovirus and feline panleukopenia virus: distinct host cell tropisms of each virus in vitro and in vivo. J Virol 1992;66: 5399-5408. https://doi.org/10.1128/jvi.66.9.5399-5408.1992
  20. Jaune FW, Taques I, Dos Santos Costa J, Araujo JP Jr, Catroxo MHB, Nakazato L, de Aguiar DM. Isolation and genome characterization of canine parvovirus type 2c in Brazil. Braz J Microbiol 2019;50:329-333. https://doi.org/10.1007/s42770-018-0036-z
  21. Sun Y, Cheng Y, Lin P, Zhang H, Yi L, Tong M, Cao Z, Li S, Cheng S, Wang J. Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis. BMC Vet Res 2019;15:141. https://doi.org/10.1186/s12917-019-1898-5
  22. Decaro N, Desario C, Miccolupo A, Campolo M, Parisi A, Martella V, Amorisco F, Lucente MS, Lavazza A, Buonavoglia C. Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis. J Gen Virol 2008;89(Pt 9):2290-2298. https://doi.org/10.1099/vir.0.2008/001503-0
  23. Battilani M, Modugno F, Mira F, Purpari G, Di Bella S, Guercio A, Balboni A. Molecular epidemiology of canine parvovirus type 2 in Italy from 1994 to 2017: recurrence of the CPV-2b variant. BMC Vet Res 2019;15:393. https://doi.org/10.1186/s12917-019-2096-1