Journal of Microbiology and Biotechnology

  • 제32권2호
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  • Pages.176-186
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  • 2022
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

  • Wu, Min (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Tang, Jie (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Zhou, Xuerui (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Lei, Dan (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Zeng, Chaoyi (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Ye, Hong (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Cai, Ting (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University) ;
  • Zhang, Qing (Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University)
  • 투고 : 2021.10.28
  • 심사 : 2022.01.12
  • 발행 : 2022.02.28
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초록

Continued fenvalerate use has caused serious environmental pollution and requires large-scale remediation. Dibutyl phthalate (DBP) was discovered in fenvalerate metabolites degraded by Citrobacter freundii CD-9. Coculturing is an effective method for bioremediation, but few studies have analyzed the degradation pathways and potential mechanisms of cocultures. Here, a DBP-degrading strain (BDBP 071) was isolated from soil contaminated with pyrethroid pesticides (PPs) and identified as Stenotrophomonas acidaminiphila. The optimum conditions for DBP degradation were determined by response surface methodology (RSM) analysis to be 30.9 mg/l DBP concentration, pH 7.5, at a culture temperature of 37.2℃. Under the optimized conditions, approximately 88% of DBP was degraded within 48 h and five metabolites were detected. Coculturing C. freundii CD-9 and S. acidaminiphila BDBP 071 promoted fenvalerate degradation. When CD-9 was cultured for 16 h before adding BDBP 071, the strain inoculation ratio was 5:5 (v/v), fenvalerate concentration was 75.0 mg/l, fenvalerate was degraded to 84.37 ± 1.25%, and DBP level was reduced by 5.21 mg/l. In addition, 12 fenvalerate metabolites were identified and a pathway for fenvalerate degradation by the cocultured strains was proposed. These results provide theoretical data for further exploration of the mechanisms used by this coculture system to degrade fenvalerate and DBP, and also offer a promising method for effective bioremediation of PPs and their related metabolites in polluted environments.

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과제정보

This study was funded by the National Natural Science Foundation of China (32102094), the Application Foundation Project of Sichuan Provincial Department of Science and Technology (2019YJ0389), the Science and Technology Support Project of Sichuan Province (No. 2019ZYZF0170), the Technological Innovation Project of Chengdu Science and Technology Bureau (2018-YF05-00522-SN), the Key Scientific Research Fund of Xihua University (Z1310525), and the Graduate Student Innovation Fund of Xihua University (ycjj2020130).

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