Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Three-Dimensional Skin Tissue Printing with Human Skin Cell Lines and Mouse Skin-Derived Epidermal and Dermal Cells

  • Jin, Soojung (Core-Facility Center for Tissue Regeneration, Dong-Eui University) ;
  • Oh, You Na (Core-Facility Center for Tissue Regeneration, Dong-Eui University) ;
  • Son, Yu Ri (Core-Facility Center for Tissue Regeneration, Dong-Eui University) ;
  • Kwon, Boguen (Core-Facility Center for Tissue Regeneration, Dong-Eui University) ;
  • Park, Jung-ha (Core-Facility Center for Tissue Regeneration, Dong-Eui University) ;
  • Gang, Min jeong (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-Eui University) ;
  • Kim, Byung Woo (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-Eui University) ;
  • Kwon, Hyun Ju (Core-Facility Center for Tissue Regeneration, Dong-Eui University)
  • Received : 2021.11.24
  • Accepted : 2021.12.13
  • Published : 2022.02.28
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Abstract

Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

Keywords

Acknowledgement

This research was supported by a Korea Basic Science Institute (National research Facilities and Equipment Center) grant funded by the Ministry of Education (2020R1A6C101A201, 2021R1A6C103B395).

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