Journal of Microbiology and Biotechnology

  • 제32권1호
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  • Pages.72-80
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  • 2022
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Multifunctional Probiotic and Functional Properties of Lactiplantibacillus plantarum LRCC5314, Isolated from Kimchi

  • Yoon, Seokmin (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Cho, Hyeokjun (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Nam, Yohan (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Park, Miri (Lotte R&D Center) ;
  • Lim, Ahyoung (Lotte R&D Center) ;
  • Kim, Jong-Hwa (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Park, Jaewoong (Lotte R&D Center) ;
  • Kim, Wonyong (Department of Microbiology, Chung-Ang University College of Medicine)
  • 투고 : 2021.09.13
  • 심사 : 2021.11.04
  • 발행 : 2022.01.28
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초록

In this study, the survival capacity (acid and bile salt tolerance, and adhesion to gut epithelial cells) and probiotic properties (enzyme activity-inhibition and anti-inflammatory activities, inhibition of adipogenesis, and stress hormone level reduction) of Lactiplantibacillus plantarum LRCC5314, isolated from kimchi (Korean traditional fermented cabbage), were investigated. LRCC5314 exhibited very stable survival at ph 2.0 and in 0.2% bile acid with 89.9% adhesion to Caco-2 intestinal epithelial cells after treatment for 2 h. LRCC5314 also inhibited the activities of α-amylase and α-glucosidase, which are involved in elevating postprandial blood glucose levels, by approximately 72.9% and 51.2%, respectively. Treatment of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells with the LRCC5314 lysate decreased the levels of the inflammatory factors nitric oxide, tumor necrosis factor (TNF-α), interleukin (IL)-1β, and interferon-γ by 88.5%, 49.3%, 97.2%, and 99.8%, respectively, relative to those of the cells treated with LPS alone. LRCC5314 also inhibited adipogenesis in differentiating preadipocytes (3T3-L1 cells), showing a 14.7% decrease in lipid droplet levels and a 74.0% decrease in triglyceride levels, as well as distinct reductions in the mRNA expression levels of adiponectin, FAS, PPAR/γ, C/EBPα, TNF-α, and IL-6. Moreover, LRCC5314 reduced the level of cortisol, a hormone with important effect on stress, by approximately 35.6% in H295R cells. L. plantarum LRCC5314 is identified as a new probiotic with excellent in vitro multifunctional properties. Subsequent in vivo studies may further demonstrate its potential as a functional food or pharmabiotic.

키워드

과제정보

The authors wish to thank LactoMason Co., Ltd. for the manufacture and standardization of bacterial strains. This work was supported by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea, as part of the (multi-ministerial) Genome Technology to Business Translation Program [no. 918004-4].

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