Journal of Microbiology and Biotechnology

  • 제32권3호
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  • Pages.378-386
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  • 2022
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Light Stress after Heterotrophic Cultivation Enhances Lutein and Biofuel Production from a Novel Algal Strain Scenedesmus obliquus ABC-009

  • Koh, Hyun Gi (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Jeong, Yong Tae (Department of Animal and Plant Research, Nakdonggang National Institute of Biological Resources (NNIBR)) ;
  • Lee, Bongsoo (Department of Microbial Biotechnology, College of Science and Technology, Mokwon University) ;
  • Chang, Yong Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST))
  • 투고 : 2021.08.17
  • 심사 : 2021.09.26
  • 발행 : 2022.03.28
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초록

Scenedesmus obliquus ABC-009 is a microalgal strain that accumulates large amounts of lutein, particularly when subjected to growth-limiting conditions. Here, the performance of this strain was evaluated for the simultaneous production of lutein and biofuels under three different modes of cultivation - photoautotrophic mode using BG-11 medium with air or 2% CO2 and heterotrophic mode using YM medium. While it was found that the highest fatty acid methyl ester (FAME) level and lutein content per biomass (%) were achieved in BG-11 medium with CO2 and air, respectively, heterotrophic cultivation resulted in much higher biomass productivity. While the cell concentrations of the cultures grown under BG-11 and CO2 were largely similar to those grown in YM medium, the disparity in the biomass yield was largely attributed to the larger cell volume in heterotrophically cultivated cells. Post-cultivation light treatment was found to further enhance the biomass productivity in all three cases and lutein content in heterotrophic conditions. Consequently, the maximum biomass (757.14 ± 20.20 mg/l/d), FAME (92.78 ± 0.08 mg/l/d), and lutein (1.006 ± 0.23 mg/l/d) productivities were obtained under heterotrophic cultivation. Next, large-scale lutein production using microalgae was demonstrated using a 1-ton open raceway pond cultivation system and a low-cost fertilizer (Eco-Sol). The overall biomass yields were similar in both media, while slightly higher lutein content was obtained using the fertilizer owing to the higher nitrogen content.

키워드

과제정보

This research was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project, funded by the Ministry of Science and ICT (ABC-2010-0029728), a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR202102101), and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT, Korea government (2021R1F1A105127511).

참고문헌

  1. Lam MK, Lee KT. 2012. Microalgae biofuels: a critical review of issues, problems and the way forward. Biotechnol. Adv. 30: 673-690. https://doi.org/10.1016/j.biotechadv.2011.11.008
  2. Torres CM, Rios SD, Torras C, Salvado J, Mateo-Sanz JM, Jimenez L. 2013. Microalgae-based biodiesel: a multicriteria analysis of the production process using realistic scenarios. Bioresour. Technol. 147: 7-16. https://doi.org/10.1016/j.biortech.2013.07.145
  3. Fasaei F, Bitter JH, Slegers PM, van Boxtel AJB. 2018. Techno-economic evaluation of microalgae harvesting and dewatering systems. Algal Res. 31: 347-362. https://doi.org/10.1016/j.algal.2017.11.038
  4. Lurling M. 1999. The smell of water: grazer-induced colony formation in Scenedesmus. Agricultural University of Wageningen.
  5. Mandal S, Mallick N. 2009. Microalga Scenedesmus obliquus as a potential source for biodiesel production. Appl. Microbiol. Biotechnol. 84: 281-291. https://doi.org/10.1007/s00253-009-1935-6
  6. Ho SH, Chen WM, Chang JS. 2010. Scenedesmus obliquus CNW-N as a potential candidate for CO2 mitigation and biodiesel production. Bioresour. Technol. 101: 8725-8730. https://doi.org/10.1016/j.biortech.2010.06.112
  7. Ho SH, Chan MC, Liu CC, Chen CY, Lee WL, Lee DJ, et al. 2014. Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies. Bioresour. Technol. 152: 275-282. https://doi.org/10.1016/j.biortech.2013.11.031
  8. Chen W-C, Hsu Y-C, Chang J-S, Ho S-H, Wang L-F, Wei Y-H. 2019. Enhancing production of lutein by a mixotrophic cultivation system using microalga Scenedesmus obliquus CWL-1. Bioresour. Technol. 291: 121891. https://doi.org/10.1016/j.biortech.2019.121891
  9. Florez-Miranda L, Canizares-Villanueva RO, Melchy-Antonio O, Martinez-Jeronimo F, Flores-Ortiz CM. 2017. Two stage heterotrophy/photoinduction culture of Scenedesmus incrassatulus: potential for lutein production. J. Biotechnol. 262: 67-74. https://doi.org/10.1016/j.jbiotec.2017.09.002
  10. Sanchez JF, Fernandez JM, Acien FG, Rueda A, Perez-Parra J, Molina E. 2008. Influence of culture conditions on the productivity and lutein content of the new strain Scenedesmus almeriensis. Process Biochem. 43: 398-405. https://doi.org/10.1016/j.procbio.2008.01.004
  11. Kijlstra A, Tian Y, Kelly ER, Berendschot TTJM. 2012. Lutein: more than just a filter for blue light. Prog. Retin. Eye Res. 31: 303-315. https://doi.org/10.1016/j.preteyeres.2012.03.002
  12. Lin JH, Lee DJ, Chang JS. 2015. Lutein production from biomass: marigold flowers versus microalgae. Bioresour. Technol. 184: 421-428. https://doi.org/10.1016/j.biortech.2014.09.099
  13. Saha SK, Ermis H, Murray P. 2020. Marine microalgae for potential lutein production. Appl. Sci. 10: 6457. https://doi.org/10.3390/app10186457
  14. Chen CY, Liu CC. 2018. Optimization of lutein production with a two-stage mixotrophic cultivation system with Chlorella sorokiniana MB-1. Bioresour. Technol. 262: 74-79. https://doi.org/10.1016/j.biortech.2018.04.024
  15. Koh HG, Kang NK, Jeon S, Shin SE, Jeong BR, Chang YK. 2019. Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol. Biofuels 12: 122. https://doi.org/10.1186/s13068-019-1462-3
  16. Tuli HS, Chaudhary P, Beniwal V, Sharma AK. 2015. Microbial pigments as natural color sources: current trends and future perspectives. J. Food Sci. Technol. 52: 4669-4678. https://doi.org/10.1007/s13197-014-1601-6
  17. Mercado I, Alvarez X, Verduga ME, Cruz A. 2020. Enhancement of biomass and lipid productivities of Scenedesmus sp. cultivated in the wastewater of the dairy industry. Processes 8: 1458. https://doi.org/10.3390/pr8111458
  18. Safi C, Zebib B, Merah O, Pontalier PY, Vaca-Garcia C. 2014. Morphology, composition, production, processing and applications of Chlorella vulgaris: a review. Renew. Sust. Energ. Rev. 35: 265-278. https://doi.org/10.1016/j.rser.2014.04.007
  19. Pegg C, Wolf M, Alanagreh La, Portman R, Buchheim MA. 2015. Morphological diversity masks phylogenetic similarity of Ettlia and Haematococcus (Chlorophyceae). Phycologia 54: 385-397. https://doi.org/10.2216/15-015.1
  20. Kamalanathan M, Chaisutyakorn P, Gleadow R, Beardall J. 2018. A comparison of photoautotrophic, heterotrophic, and mixotrophic growth for biomass production by the green alga Scenedesmus sp. (Chlorophyceae). Phycologia 57: 309-317. https://doi.org/10.2216/17-82.1
  21. Kim DG, Hur SB. 2013. Growth and fatty acid composition of three heterotrophic Chlorella species. Algae 28: 101-109. https://doi.org/10.4490/algae.2013.28.1.101
  22. Aligata AJ, Tryner J, Quinn JC, Marchese AJ. 2019. Effect of microalgae cell composition and size on responsiveness to ultrasonic harvesting. J. Appl. Phycol. 31: 1637-1649. https://doi.org/10.1007/s10811-018-1682-0
  23. Jeon S, Kang NK, Suh WI, Koh HG, Lee B, Chang YK. 2019. Optimization of electroporation-based multiple pulses and further improvement of transformation efficiency using bacterial conditioned medium for Nannochloropsis salina. J. Appl. Phycol. 31: 1153-1161. https://doi.org/10.1007/s10811-018-1599-7
  24. Poh ZL, Amalina Kadir WN, Lam MK, Uemura Y, Suparmaniam U, Lim JW, et al. 2020. The effect of stress environment towards lipid accumulation in microalgae after harvesting. Renew. Energy 154: 1083-1091. https://doi.org/10.1016/j.renene.2020.03.081
  25. Shokravi Z, Shokravi H, Chyuan OH, Lau WJ, Koloor SSR, Petru M, et al. 2020. Improving 'lipid productivity' in microalgae by bilateral enhancement of biomass and lipid contents: a review. Sustainability 12: 9083. https://doi.org/10.3390/su12219083
  26. Minhas AK, Hodgson P, Barrow CJ, Sashidhar B, Adholeya A. 2016. The isolation and identification of new microalgal strains producing oil and carotenoid simultaneously with biofuel potential. Bioresour. Technol. 211: 556-565. https://doi.org/10.1016/j.biortech.2016.03.121
  27. Ram S, Paliwal C, Mishra S. 2019. Growth medium and nitrogen stress sparked biochemical and carotenogenic alterations in Scenedesmus sp. CCNM 1028. Bioresour. Technol. Rep. 7: 100194. https://doi.org/10.1016/j.biteb.2019.100194
  28. Batista AP, Moura P, Marques P, Ortigueira J, Alves L, Gouveia L. 2014. Scenedesmus obliquus as feedstock for biohydrogen production by Enterobacter aerogenes and Clostridium butyricum. Fuel 117: 537-543. https://doi.org/10.1016/j.fuel.2013.09.077
  29. Gouveia L, Oliveira AC. 2009. Microalgae as a raw material for biofuels production. J. Ind. Microbiol. Biotechnol. 36: 269-74. https://doi.org/10.1007/s10295-008-0495-6
  30. Feng P, Yang K, Xu Z, Wang Z, Fan L, Qin L, et al. 2014. Growth and lipid accumulation characteristics of Scenedesmus obliquus in semi-continuous cultivation outdoors for biodiesel feedstock production. Bioresour. Technol. 173: 406-414. https://doi.org/10.1016/j.biortech.2014.09.123
  31. Chan MC, Ho SH, LeeDJ, Chen CY, Huang CC, Chang JS. 2013. Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N. Biochem. Eng. J. 78: 24-31. https://doi.org/10.1016/j.bej.2012.11.017
  32. Xie Y, Zhao X, Chen J, Yang X, Ho SH, Wang B, et al. 2017. Enhancing cell growth and lutein productivity of Desmodesmus sp. F51 by optimal utilization of inorganic carbon sources and ammoniumsalt. Bioresour. Technol. 244: 664-671. https://doi.org/10.1016/j.biortech.2017.08.022
  33. Chen CY, Ho SH, Liu CC, Chang JS. 2017. Enhancing lutein production with Chlorella sorokiniana Mb-1 byoptimizing acetate and nitrate concentrations under mixotrophic growth. J. Taiwan Inst. Chem. Eng. 79: 88-96. https://doi.org/10.1016/j.jtice.2017.04.020