Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Appropriate Soil Heat Treatment Promotes Growth and Disease Suppression of Panax notoginseng by Interfering with the Bacterial Community

  • Li, Ying-Bin (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Zhang, Zhi-Ping (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Yuan, Ye (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Huang, Hui-Chuan (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Mei, Xin-Yue (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Du, Fen (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Yang, Min (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Liu, Yi-Xiang (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University) ;
  • Zhu, Shu-Sheng (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University)
  • Received : 2021.12.02
  • Accepted : 2022.02.15
  • Published : 2022.03.28
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Abstract

In our greenhouse experiment, soil heat treatment groups (50, 80, and 121℃) significantly promoted growth and disease suppression of Panax notoginseng in consecutively cultivated soil (CCS) samples (p < 0.01), and 80℃ worked better than 50℃ and 121℃ (p < 0.01). Furthermore, we found that heat treatment at 80℃ changes the microbial diversity in CCS, and the inhibition ratios of culturable microorganisms, such as fungi and actinomycetes, were nearly 100%. However, the heat-tolerant bacterial community was preserved. The 16S rRNA gene and internal transcribed spacer (ITS) sequencing analyses indicated that the soil heat treatment had a greater effect on the Chao1 index and Shannon's diversity index of bacteria than fungi, and the relative abundances of Firmicutes and Proteobacteria were significantly higher than without heating (80 and 121℃, p < 0.05). Soil probiotic bacteria, such as Bacillus (67%), Sporosarcina (9%), Paenibacillus (6%), Paenisporosarcina (6%), and Cohnella (4%), remained in the soil after the 80℃ and 121℃ heat treatments. Although steam increased the relative abundances of most of the heat-tolerant microbes before sowing, richness and diversity gradually recovered to the level of CCS, regardless of fungi or bacteria, after replanting. Thus, we added heat-tolerant microbes (such as Bacillus) after steaming, which reduced the relative abundance of pathogens, recruited antagonistic bacteria, and provided a long-term protective effect compared to the steaming and Bacillus alone (p < 0.05). Taken together, the current study provides novel insight into sustainable agriculture in a consecutively cultivated system.

Keywords

Acknowledgement

This work was supported by the National Key R&D Program of China (2021YFD1000200), the Major Science and Technology Project in Yunnan Province (202102AE090042), the Major Science and Technology Project in Kunming (2021-J-H-002), the Innovative Research Team of Science and Technology in Yunnan Province (202105AE160016), the Expert Workstation Project in Yunnan Province (202005AF150103), and the Yunnan provincial key programs of Yunnan Eco-friendly Food International Cooperation Research Center project (2019ZG00901).

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