Journal of Microbiology and Biotechnology

  • 제32권3호
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  • Pages.355-364
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  • 2022
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Lactic Acid Bacteria from Gamecock and Goat Originating from Phitsanulok, Thailand: Isolation, Identification, Technological Properties and Probiotic Potential

  • Hwanhlem, Noraphat (Division of Animal Science and Feed Technology, Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University) ;
  • Salaipeth, Lakha (School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Charoensook, Rangsun (Division of Animal Science and Feed Technology, Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University) ;
  • Kanjan, Pochanart (Department of Agricultural and Fishery Science, Faculty of Science and Technology, Prince of Songkla University) ;
  • Maneerat, Suppasil (Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University)
  • 투고 : 2021.10.25
  • 심사 : 2022.01.11
  • 발행 : 2022.03.28
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초록

From independent swab samples of the cloaca of indigenous gamecocks (CIG), anus of healthy baby goats (AHG), and vagina of goats (VG) originating from Phitsanulok, Thailand, a total of 263 isolates of lactic acid bacteria (LAB) were collected. Only three isolates, designated C707, G502, and V202, isolated from CIG, AHG, and VG, respectively, exhibited an excellent inhibitory zone diameter against foodborne pathogenic bacteria when evaluated by agar spot test. Isolates C707 and G502 were identified as Enterococcus faecium, whereas V202 was identified as Pediococcus acidilactici, based on 16S rRNA sequence analysis. When foodborne pathogenic bacteria were co-cultured with chosen LAB in mixed BHI-MRS broth at 39℃, their growth was suppressed. These LAB were found to be capable of surviving in simulated stomach conditions. Only the isolate G502 was able to survive in the conditions of simulated intestinal juice. This research suggests that selected LAB could be used as a food/feed supplement to reduce foodborne pathogenic bacteria and improve the safety of animal-based food or feed.

키워드

과제정보

Authors thank Kittisak Thampitak, Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, for his technical and research assistance.

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