한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제50권1호
  • /
  • Pages.63-70
  • /
  • 2022
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240

  • Le, Huong Giang (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kim, Min Jae (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Jeon, Hye Sung (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Yoo, Ji Yeon (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kang, Yun Ji (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kim, Tae Jin (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kim, Jeong Hwan (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University)
  • 투고 : 2022.01.12
  • 심사 : 2022.02.22
  • 발행 : 2022.03.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria-Escherichia coli shuttle vectors.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1A2C1008267). Le HG, Kim MJ, and Kim TJ were supported by full-time graduate student scholarship from Gyeongsang National University. Jeon HS, Yoo JY, and Kang YJ were supported by BK21 Four program from MOE, Korea.

참고문헌

  1. Mokoena MP. 2017. Lactic acid bacteria and their bacteriocins: classification, biosynthesis and applications against uropathogens: a mini-review. Molecules 22: 1255. https://doi.org/10.3390/molecules22081255
  2. Cui Y, Hu T, Qu X, Zhang L, Ding Z, Dong A. 2015. Plasmids from food lactic acid bacteria: diversity, similarity, and new developments. Int. J. Mol. Sci. 16: 13172-13202. https://doi.org/10.3390/ijms160613172
  3. Mikelsaar M, Mandar R, Sepp E, Annuk H. 2004. Human lactic acid microflora and its role in the welfare of the host, pp. 453-506. In Salminen S, von Wright A, Ouwe-hand A (eds.), Lactic Acid Bacteria, 3rd Ed. CRC Press, Boca Raton.
  4. Bintsis T. 2018. Lactic acid bacteria: their applications in foods. J. Bacteriol. Mycol. 6: 89-94.
  5. Hatti-Kaul R, Chen L, Dishisha T, El Enshasy H. 2018. Lactic acid bacteria: from starter culltures to producers of chemicals. FEMS Microbiol. Lett. 365: doi: 10.1093/femsle/fny213.
  6. Sharma A, Gupta G, Ahmad T, Kaul B, Hakeem KR. 2020. Tailoring cellular metabolism in lactic acid bacteria through metabolic engineering. J. Microbiol. Methods 170: 105862. https://doi.org/10.1016/j.mimet.2020.105862
  7. Kleerebezem M, Hols P, Hugenholtz J. 2000. Lactic acid bacteria as a cell factory: rerouting of carbon metabolism in Lactococcus lactis by metabolic engineering. Enzyme Microb. Technol. 26: 840-848. https://doi.org/10.1016/S0141-0229(00)00180-0
  8. Mierau I, Kleerebezem. 2005. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl. Microbiol. Biotechnol. 68: 705-717. https://doi.org/10.1007/s00253-005-0107-6
  9. Jeong SJ, Park JY, Lee HJ, Kim JH. 2007. Characterization of pFMBL1, a small cryptic plasmid isolated from Leuconostoc mesenteroides SY2. Plasmid 57: 314-323. https://doi.org/10.1016/j.plasmid.2006.09.003
  10. Park JY, Jeong SJ, Sa HD, Lee JY, Liu X, Cho MJ, et al. 2015. Construction of a shuttle vector based on the small cryptic plasmid pJY33 from Weissella cibaria 33. Plasmid 79: 30-36. https://doi.org/10.1016/j.plasmid.2015.03.008
  11. Park JY, Jeong SJ, Kim JH. 2014. Characterization of a glutamate decarboxylase (GAD) gene from Lactobacillus zymae. Biotechnol. Lett. 36: 1791-1799. https://doi.org/10.1007/s10529-014-1539-9
  12. O'Sullivan DJ, Klaenhammer TR. 1993. Rapid mini-prep isolation of high-quality plasmid DNA from Lactococcus and Lactobacillus spp. Appl. Environ. Microbiol. 59: 2730-2733. https://doi.org/10.1128/aem.59.8.2730-2733.1993
  13. Sambrook JF, Russell DW. 2001. Molecular cloning: a laboratory manual. 3rd edition. Cold Spring Harbor Laboratory Press, New York.
  14. Zhu X, Zhao Y, Zhang C, Shen L, Jiang H, Gu Q. 2015. Characterization of three new plasmids from Lactobacillus paracasei 54. Turk. J. Biochem. 40: 265-270. https://doi.org/10.1515/tjb-2015-0008
  15. Pittet V, Abegunde T, Marfleet T, Haakensen M, Morrow K, Jayaprakash T, et al. 2012. Complete genome sequence of the beer spoilage organism Pediococcus claussenii ATCC BAA-344T. J. Bacteriol. 194: 1271-1272. https://doi.org/10.1128/JB.06759-11
  16. Coram NJ, van Zyl LJ, Rawlings DE. 2005. Isolation, sequence analysis, and comparision of two plasmids (28 and 29 kilobases) from the biomining bacterium Leptospirillum ferrooxidans ATCC 49879. Appl. Environ. Microbiol. 71: 7515-7522. https://doi.org/10.1128/AEM.71.11.7515-7522.2005
  17. McLeod A, Fagerlund A, Rud I, Axelsson L. 2019. Large plasmid complement resolved: complete genome sequencing of Lactobacillus plantarum MF1298, a candidate probiotic strain associated with unfavorable effect. Microorganisms 7: 262. https://doi.org/10.3390/microorganisms7080262
  18. Viret J-F, Bravo A, Alonso JC. 1991. Recombination-dependent concatemeric plasmid replication. Microbiol. Rev. 55: 675-683. https://doi.org/10.1128/mr.55.4.675-683.1991
  19. Ruiz-Maso, Machon C, Boedanaba-Ruiseco L, Espinosa M, Coll M, Del Solar G. 2015. Plasmid rolling-circle replication. Microbiol. Spectr. 3: PLAS-0035-2014.
  20. Kaur T, Balgir PP, Kaur B. 2019. Construction of a shuttle expression vector for lactic acid bacteria. J. Genet. Eng. Biotechnol. 17: 10. https://doi.org/10.1186/s43141-019-0013-4