Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mycorrhizal Synthesis of Périgord Black Truffle (Tuber melanosporum) with Mexican Oak Species

  • Received : 2021.09.26
  • Accepted : 2022.01.17
  • Published : 2022.03.28
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Abstract

The Périgord black truffle (Tuber melanosporum) is an edible fungus and among the most expensive foods worldwide. It is the basis of a multimillion-dollar bio-business. Truffle farming does not exist in Mexico, and no formal studies have been conducted on its culture. This report describes the mycorrhizal synthesis (i.e., artificial union of fungus with host) of T. melanosporum with oak species native to Mexico (Quercus polymorpha, Q. fusiformis, and Q. canbyi). The mycorrhizal association was successful in Q. polymorpha and Q. fusiformis, as confirmed morphologically and using T. melanosporum molecular primers (ITSML/ITS4LNG). The effect of the ectomycorrhizal fungus on host growth (stem diameter) was statistically significant. Illustrations of the study are presented.

Keywords

Acknowledgement

Guevara-Guerrero thanks CONACyT and TecNM for economic and research support. The senior author acknowledges Ana Maria Mejia Garcia for her encouragement to perform this research. Victor A. Vargas Vazquez is appreciated for research support.

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