Journal of Applied Biological Chemistry

  • 제65권1호
  • /
  • Pages.33-41
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  • 2022
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Anti-inflammatory activity of 6-O-phospho-7-hydroxycoumarin in LPS-induced RAW 264.7 cells

  • Hong, Hyehyun (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Park, Tae-Jin (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Jang, Sungchan (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kim, Min-Seon (Natural Product Informatics Research Center, Korea Institute of Science and Technology) ;
  • Park, Jin-Soo (Natural Product Informatics Research Center, Korea Institute of Science and Technology) ;
  • Chi, Won-Jae (Microorganism Resources Division, Biological Resources Research Department, National Institute of Biological Resources) ;
  • Kim, Seung-Young (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
  • 투고 : 2021.12.08
  • 심사 : 2022.01.20
  • 발행 : 2022.03.31
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초록

Esculetin (also known as 6, 7-dihydroxycoumarin) a type of coumarin, has been exhibited anti-inflammatory and anti-aging effects. Biorenovation is the microbe-mediated enhancement of biological efficacies and structurally diversified compounds relative to their substrate compounds. The production of different kinds of esculetin derivatives using Bacillus sp. JD3-7 and their effects on lipopolysaccharide (LPS)-triggered inflammatory response in RAW 26.7 cells were assessed. One of the biorenovation products, identified as esculetin 6-O-phosphate (ESP), at concentrations of 1.25, 2.5, and 5 μM inhibited the LPS-stimulated production of inflammation markers of nitric oxide synthase 2 and cyclooxygenase 2 as well as their respective enzymatic reaction products of nitric oxide and prostaglandin E2 in the order of increasing concentrations (1.25, 2.5, and 5 μM). Additionally, ESP treatment suppressed the LPS-stimulated secretion of pro-inflammatory cytokines of interleukin (IL)-1β, IL-6, and tumor necrosis factor- α. Furthermore, these anti-inflammatory effect of ESP was associated with the downregulation of mitogen-activated protein kinase signaling, that is, extracellular signal-regulated kinase, c-Jun NH2-terminal kinase, and p38 mitogen-activated protein kinase signaling pathways. This study would therefore provide interesting insights into the biorenovation-assisted generation of a novel anti-inflammatory compound. ESP may be used to develop treatments for inflammatory disorders.

키워드

과제정보

This research was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202102109).

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